Gene description for STON2
Gene name stonin 2
Gene symbol STON2
Other names/aliases STN2
STNB
STNB2
Species Homo sapiens
 Database cross references - STON2
ExoCarta ExoCarta_85439
Vesiclepedia VP_85439
Entrez Gene 85439
HGNC 30652
MIM 608467
UniProt Q8WXE9  
 STON2 identified in sEVs derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for STON2
Molecular Function
    protein binding GO:0005515 IPI
    clathrin adaptor activity GO:0035615 IBA
Biological Process
    regulation of endocytosis GO:0030100 IBA
    regulation of endocytosis GO:0030100 ISS
    synaptic vesicle endocytosis GO:0048488 IBA
    synaptic vesicle endocytosis GO:0048488 IGI
    clathrin-dependent endocytosis GO:0072583 IBA
Subcellular Localization
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    synaptic vesicle GO:0008021 IBA
    AP-2 adaptor complex GO:0030122 IBA
    neuron projection GO:0043005 IEA
 Experiment description of studies that identified STON2 in sEVs
1
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for STON2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAM1 7412
Affinity Capture-MS Homo sapiens
2 TOMM40 10452
Co-fractionation Homo sapiens
3 ANLN 54443
Affinity Capture-MS Homo sapiens
4 GTSE1 51512
Affinity Capture-MS Homo sapiens
5 LIMA1 51474
Affinity Capture-MS Homo sapiens
6 AP2B1 163
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 Sec24c  
Affinity Capture-MS Mus musculus
8 PPP1CB 5500
Affinity Capture-MS Homo sapiens
9 VDAC3 7419
Co-fractionation Homo sapiens
10 TOR1A 1861
Affinity Capture-Western Homo sapiens
11 LAMTOR1 55004
Proximity Label-MS Homo sapiens
12 HADHB 3032
Co-fractionation Homo sapiens
13 CLINT1 9685
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 Myh9 17886
Affinity Capture-MS Mus musculus
15 Lima1  
Affinity Capture-MS Mus musculus
16 KIF14 9928
Affinity Capture-MS Homo sapiens
17 AP2S1 1175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ATG9A 79065
Proximity Label-MS Homo sapiens
19 Tpm1 22003
Affinity Capture-MS Mus musculus
20 SYT1 6857
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
21 RACGAP1 29127
Affinity Capture-MS Homo sapiens
22 Calml3  
Affinity Capture-MS Mus musculus
23 SYT2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
24 Myo1c 17913
Affinity Capture-MS Mus musculus
25 DBN1 1627
Affinity Capture-MS Homo sapiens
26 EPS15 2060
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
27 CAPZA2 830
Affinity Capture-MS Homo sapiens
28 TOMM22 56993
Co-fractionation Homo sapiens
29 ABL1 25
Affinity Capture-MS Homo sapiens
30 H19 283120
Protein-RNA Homo sapiens
31 MYO19  
Affinity Capture-MS Homo sapiens
32 Actb 11461
Affinity Capture-MS Mus musculus
33 CLTB 1212
Affinity Capture-MS Homo sapiens
34 C11orf52 91894
Proximity Label-MS Homo sapiens
35 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 FN1 2335
Affinity Capture-MS Homo sapiens
37 Myh10 77579
Affinity Capture-MS Mus musculus
38 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
39 SYNPO 11346
Affinity Capture-MS Homo sapiens
40 RNPS1 10921
Affinity Capture-MS Homo sapiens
41 MYC  
Affinity Capture-MS Homo sapiens
42 Uso1 56041
Affinity Capture-MS Mus musculus
43 MYH9 4627
Affinity Capture-MS Homo sapiens
44 Tmod3 50875
Affinity Capture-MS Mus musculus
45 FLNA 2316
Affinity Capture-MS Homo sapiens
46 ZCCHC10  
Affinity Capture-MS Homo sapiens
47 CLTA 1211
Proximity Label-MS Homo sapiens
48 AP1B1 162
Affinity Capture-MS Homo sapiens
49 Flot1 14251
Affinity Capture-MS Mus musculus
50 PRC1 9055
Affinity Capture-MS Homo sapiens
51 KRAS 3845
Proximity Label-MS Homo sapiens
52 RHOB 388
Proximity Label-MS Homo sapiens
53 CLTC 1213
Affinity Capture-MS Homo sapiens
54 ITSN1 6453
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
55 COPS4 51138
Affinity Capture-Western Homo sapiens
56 C15orf57  
Affinity Capture-MS Homo sapiens
57 Ppp1cb 19046
Affinity Capture-MS Mus musculus
58 LSR 51599
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 PICALM 8301
Affinity Capture-MS Homo sapiens
60 EPS15L1 58513
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
61 VDAC2 7417
Co-fractionation Homo sapiens
62 GAK 2580
Affinity Capture-MS Homo sapiens
63 MYO18A 399687
Affinity Capture-MS Homo sapiens
64 EP300 2033
Affinity Capture-MS Homo sapiens
65 ITGA4 3676
Affinity Capture-MS Homo sapiens
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