Experiment description of studies that identified CDK3 in sEVs

1 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

2 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for CDK3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CCNO   Affinity Capture-MS Homo sapiens 2 APP 351 Reconstituted Complex Homo sapiens 3 CDC25A   Affinity Capture-MS Homo sapiens 4 CUL1 8454 Affinity Capture-MS Homo sapiens 5 LHX4   Two-hybrid Homo sapiens 6 CCNE2   Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 7 ARRB2 409 Affinity Capture-MS Homo sapiens 8 KLRC3   Affinity Capture-MS Homo sapiens 9 FOXP2 93986 Two-hybrid Homo sapiens 10 CCNA2 890 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 CCNH   Two-hybrid Homo sapiens 12 AKT2 208 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 CCNC   Two-hybrid Homo sapiens 14 RB1 5925 Biochemical Activity Homo sapiens 15 HSPA8 3312 Affinity Capture-MS Homo sapiens 16 CABLES2   Affinity Capture-Western Homo sapiens 17 CCNE1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 RACGAP1 29127 Two-hybrid Homo sapiens 19 FAM35A   Proximity Label-MS Homo sapiens 20 SKP1 6500 Affinity Capture-MS Homo sapiens 21 EIF2AK2 5610 Affinity Capture-MS Homo sapiens 22 HSP90AB4P 664618 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 PAX8   Two-hybrid Homo sapiens 24 CDK5R1   Affinity Capture-Western Homo sapiens 25 LCK 3932 Proximity Label-MS Homo sapiens 26 HSP90AB1 3326 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 SKP2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 CDT1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 CDC25B 994 Affinity Capture-MS Homo sapiens 30 ISG15 9636 Affinity Capture-MS Homo sapiens 31 NELFA   Two-hybrid Homo sapiens 32 CDKN1A   Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 CDK5 1020 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 34 HSP90AA1 3320 Affinity Capture-Luminescence Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 HSP90AA5P 730211 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 OR2G6   Two-hybrid Homo sapiens 37 CKS1B   Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 38 CDK1 983 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 39 TRIM55   Two-hybrid Homo sapiens 40 CCNI 10983 Two-hybrid Homo sapiens 41 PAX5   Two-hybrid Homo sapiens 42 CDK2 1017 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 43 FKBP5 2289 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 44 TFDP1   Affinity Capture-Western Homo sapiens 45 CCNB1 891 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 46 SNIP1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 47 B3GNT2 10678 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 48 GMNN   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 49 PPARA   Two-hybrid Homo sapiens 50 IGF1R 3480 Affinity Capture-MS Homo sapiens 51 FRS2 10818 Two-hybrid Homo sapiens 52 COPS6 10980 Affinity Capture-MS Homo sapiens 53 LHX3   Two-hybrid Homo sapiens 54 TRIM63   Two-hybrid Homo sapiens 55 HSPA1L 3305 Affinity Capture-MS Homo sapiens 56 EEF1A2 1917 Affinity Capture-MS Homo sapiens 57 COPS5 10987 Affinity Capture-MS Homo sapiens 58 MEOX2   Two-hybrid Homo sapiens 59 SERBP1 26135 Affinity Capture-MS Homo sapiens 60 SNW1 22938 Affinity Capture-MS Homo sapiens 61 SPDYE4   Two-hybrid Homo sapiens 62 CKS2   Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 63 STK4 6789 Affinity Capture-MS Homo sapiens 64 CDKN1B 1027 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 65 HSPA4 3308 Affinity Capture-MS Homo sapiens 66 CDKN1C   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 67 STIP1 10963 Affinity Capture-MS Homo sapiens 68 NEDD4L 23327 Reconstituted Complex Homo sapiens 69 STK3 6788 Affinity Capture-MS Homo sapiens 70 CCND3   Two-hybrid Homo sapiens 71 OTX2   Two-hybrid Homo sapiens 72 OBFC1 79991 Two-hybrid Homo sapiens View the network

Pathways in which CDK3 is involved

No pathways found