Gene description for ANAPC4
Gene name anaphase promoting complex subunit 4
Gene symbol ANAPC4
Other names/aliases APC4
Species Homo sapiens
 Database cross references - ANAPC4
ExoCarta ExoCarta_29945
Vesiclepedia VP_29945
Entrez Gene 29945
HGNC 19990
MIM 606947
UniProt Q9UJX5  
 ANAPC4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ANAPC4
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 TAS
    protein binding GO:0005515 IPI
    protein phosphatase binding GO:0019903 IPI
Biological Process
    regulation of mitotic cell cycle GO:0007346 NAS
    regulation of mitotic metaphase/anaphase transition GO:0030071 IEA
    anaphase-promoting complex-dependent catabolic process GO:0031145 IBA
    anaphase-promoting complex-dependent catabolic process GO:0031145 IDA
    anaphase-promoting complex-dependent catabolic process GO:0031145 NAS
    cell division GO:0051301 IEA
    regulation of meiotic cell cycle GO:0051445 NAS
    protein K48-linked ubiquitination GO:0070936 IDA
    protein K11-linked ubiquitination GO:0070979 IBA
    protein K11-linked ubiquitination GO:0070979 IDA
    protein branched polyubiquitination GO:0141198 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    anaphase-promoting complex GO:0005680 IBA
    anaphase-promoting complex GO:0005680 IDA
    anaphase-promoting complex GO:0005680 IPI
    anaphase-promoting complex GO:0005680 NAS
    cytosol GO:0005829 TAS
    nuclear periphery GO:0034399 IBA
 Experiment description of studies that identified ANAPC4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ANAPC4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
2 CDC26 246184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 Cdk1 12534
Affinity Capture-MS Mus musculus
4 Anapc13  
Affinity Capture-MS Mus musculus
5 UBL4A 8266
Affinity Capture-MS Homo sapiens
6 PAXIP1  
Affinity Capture-MS Homo sapiens
7 DHCR24 1718
Affinity Capture-MS Homo sapiens
8 BUB1  
Affinity Capture-MS Homo sapiens
9 RACGAP1 29127
Affinity Capture-MS Homo sapiens
10 ANAPC5 51433
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ANAPC15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
12 BUB1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
13 Sirt2 64383
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
14 PYCR1 5831
Affinity Capture-MS Homo sapiens
15 TERF2IP 54386
Affinity Capture-MS Homo sapiens
16 EIF1 10209
Affinity Capture-MS Homo sapiens
17 CDC16 8881
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 DYRK1A 1859
Affinity Capture-MS Homo sapiens
19 CFAP97  
Affinity Capture-MS Homo sapiens
20 GPHN 10243
Affinity Capture-MS Homo sapiens
21 FZR1  
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
22 MDC1  
Affinity Capture-MS Homo sapiens
23 KLHL7  
Affinity Capture-MS Homo sapiens
24 POU5F1  
Affinity Capture-MS Homo sapiens
25 SRP14 6727
Affinity Capture-MS Homo sapiens
26 ANAPC11  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CDC27 996
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 FBXO5  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
29 ANAPC7 51434
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
30 KNDC1  
Affinity Capture-MS Homo sapiens
31 ANAPC16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 MAD2L1 4085
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
33 Cdc20  
Affinity Capture-MS Mus musculus
34 USP37  
Proximity Label-MS Homo sapiens
35 GPR156  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 XPO1 7514
Affinity Capture-MS Homo sapiens
37 SRP9 6726
Affinity Capture-MS Homo sapiens
38 Anapc5 59008
Affinity Capture-MS Mus musculus
39 CDC23 8697
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 BAX 581
Affinity Capture-MS Homo sapiens
41 TUBA1A 7846
Affinity Capture-MS Homo sapiens
42 Cxxc5  
Affinity Capture-MS Mus musculus
43 UBE2I 7329
Biochemical Activity Homo sapiens
44 TUBB2B 347733
Affinity Capture-MS Homo sapiens
45 CCNB1 891
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
46 NEK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
47 Gm9174  
Affinity Capture-MS Mus musculus
48 TTLL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 PCDHA4 56144
Affinity Capture-MS Homo sapiens
50 ANAPC4 29945
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
51 LYST 1130
Affinity Capture-MS Homo sapiens
52 RAD21 5885
Affinity Capture-Western Homo sapiens
53 KIF2C 11004
Affinity Capture-MS Homo sapiens
54 KIF18A  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
55 TUBA3C 7278
Affinity Capture-MS Homo sapiens
56 UBE2S  
Reconstituted Complex Homo sapiens
57 ANAPC13 25847
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 ANO6 196527
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 CCNA2 890
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
60 COPS2 9318
Affinity Capture-MS Homo sapiens
61 COPS7A 50813
Affinity Capture-MS Homo sapiens
62 Cdc23  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
63 TP53BP1 7158
Affinity Capture-MS Homo sapiens
64 TRIM33 51592
Affinity Capture-Western Homo sapiens
65 SMG8 55181
Affinity Capture-MS Homo sapiens
66 ANAPC10  
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 PSMD8 5714
Affinity Capture-Western Homo sapiens
68 CUL3 8452
Affinity Capture-MS Homo sapiens
69 PTEN 5728
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
70 TCL1A  
Affinity Capture-MS Homo sapiens
71 DCPS 28960
Co-purification Homo sapiens
72 PYCR2 29920
Affinity Capture-MS Homo sapiens
73 Anapc16  
Affinity Capture-MS Mus musculus
74 Cdc16  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
75 CUL9  
Affinity Capture-MS Homo sapiens
76 HECW2  
Affinity Capture-MS Homo sapiens
77 FAM91A1 157769
Affinity Capture-MS Homo sapiens
78 HP1BP3 50809
Affinity Capture-MS Homo sapiens
79 Anapc1  
Affinity Capture-MS Mus musculus
80 Bub1b 12236
Affinity Capture-MS Mus musculus
81 Mad2l1 56150
Affinity Capture-MS Mus musculus
82 NANOG  
Affinity Capture-MS Homo sapiens
83 ACBD5 91452
Proximity Label-MS Homo sapiens
84 C6orf222  
Affinity Capture-MS Homo sapiens
85 CXorf66  
Affinity Capture-MS Homo sapiens
86 DEPDC1B 55789
Affinity Capture-MS Homo sapiens
87 Anapc2  
Affinity Capture-MS Mus musculus
88 RRM2 6241
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 CDC20 991
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
90 SIRT6  
Affinity Capture-MS Homo sapiens
91 SKP1 6500
Affinity Capture-MS Homo sapiens
92 ANAPC1 64682
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 DCAF11  
Affinity Capture-MS Homo sapiens
94 NUP35 129401
Affinity Capture-MS Homo sapiens
95 BUB3 9184
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
96 TUBB4A 10382
Affinity Capture-MS Homo sapiens
97 CDK1 983
Affinity Capture-Western Homo sapiens
98 CCT7 10574
Affinity Capture-MS Homo sapiens
99 MAGIX 79917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 ANAPC2 29882
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 PENK 5179
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 PSMA5 5686
Affinity Capture-MS Homo sapiens
103 KIF18B 146909
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
104 FHOD3  
Affinity Capture-MS Homo sapiens
105 BRD1 23774
Affinity Capture-MS Homo sapiens
106 KRAS 3845
Synthetic Lethality Homo sapiens
107 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
108 HSP90AB4P 664618
Affinity Capture-MS Homo sapiens
109 PHF8  
Affinity Capture-MS Homo sapiens
110 RPA3 6119
Proximity Label-MS Homo sapiens
111 C16orf87 388272
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 KIF4A 24137
Affinity Capture-MS Homo sapiens
113 Eea1  
Affinity Capture-MS Mus musculus
114 HIST1H2AE 3012
Affinity Capture-MS Homo sapiens
115 TIMM13 26517
Affinity Capture-MS Homo sapiens
116 Cdc26  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which ANAPC4 is involved
PathwayEvidenceSource
Aberrant regulation of mitotic cell cycle due to RB1 defects TAS Reactome
Aberrant regulation of mitotic exit in cancer due to RB1 defects TAS Reactome
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins TAS Reactome
Adaptive Immune System TAS Reactome
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome
APC-Cdc20 mediated degradation of Nek2A TAS Reactome
APC/C-mediated degradation of cell cycle proteins TAS Reactome
APC/C:Cdc20 mediated degradation of Cyclin B TAS Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins TAS Reactome
APC/C:Cdc20 mediated degradation of Securin TAS Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 TAS Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint TAS Reactome
Assembly of the pre-replicative complex TAS Reactome
Autodegradation of Cdh1 by Cdh1:APC/C TAS Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A TAS Reactome
CDK-mediated phosphorylation and removal of Cdc6 TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Class I MHC mediated antigen processing & presentation TAS Reactome
Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase TAS Reactome
Disease TAS Reactome
Diseases of mitotic cell cycle TAS Reactome
DNA Replication TAS Reactome
DNA Replication Pre-Initiation TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Immune System TAS Reactome
Inactivation of APC/C via direct inhibition of the APC/C complex TAS Reactome
Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components TAS Reactome
M Phase TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Spindle Checkpoint TAS Reactome
Phosphorylation of the APC/C TAS Reactome
Regulation of APC/C activators between G1/S and early anaphase TAS Reactome
Regulation of mitotic cell cycle TAS Reactome
RNA Polymerase II Transcription TAS Reactome
S Phase TAS Reactome
Senescence-Associated Secretory Phenotype (SASP) TAS Reactome
Separation of Sister Chromatids TAS Reactome
Switching of origins to a post-replicative state TAS Reactome
Synthesis of DNA TAS Reactome
Transcriptional Regulation by VENTX TAS Reactome





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