Experiment description of studies that identified Shcbp1 in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 15 MISEV standards ✔ EM Biophysical techniques ✔ CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

3 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Shcbp1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 RACGAP1 29127 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 2 APOBEC3B 9582 Affinity Capture-MS Homo sapiens 3 ATAD3A 55210 Affinity Capture-MS Homo sapiens 4 YME1L1 10730 Affinity Capture-MS Homo sapiens 5 TPM1 7168 Affinity Capture-MS Homo sapiens 6 SHCBP1 79801 Affinity Capture-MS Homo sapiens 7 SLC1A5 6510 Affinity Capture-MS Homo sapiens 8 GPC5 2262 Affinity Capture-MS Homo sapiens 9 MICAL3 57553 Affinity Capture-MS Homo sapiens 10 KIF23 9493 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 MYL6 4637 Affinity Capture-MS Homo sapiens 12 DDX49 54555 Affinity Capture-MS Homo sapiens 13 TRAM1 23471 Affinity Capture-MS Homo sapiens 14 DERL1 79139 Affinity Capture-MS Homo sapiens 15 KLHL7   Affinity Capture-MS Homo sapiens 16 Trim71   Two-hybrid Mus musculus Affinity Capture-Western Mus musculus 17 ACOT9 23597 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 PDIA4 9601 Affinity Capture-MS Homo sapiens View the network

Pathways in which Shcbp1 is involved

No pathways found