Gene ontology annotations for PSMA6
Experiment description of studies that identified PSMA6 in sEVs
1
Experiment ID
79
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors
"Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
2
Experiment ID
80
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors
"Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
3
Experiment ID
81
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors
"Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
4
Experiment ID
489
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
490
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
6
Experiment ID
491
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
7
Experiment ID
492
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
8
Experiment ID
412
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MCF7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
9
Experiment ID
414
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
10
Experiment ID
426
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
11
Experiment ID
427
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
12
Experiment ID
494
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
Osteoarthritic cartilage
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
13
Experiment ID
496
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
Healthy cartilage
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
14
Experiment ID
497
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
15
Experiment ID
498
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
16
Experiment ID
207
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
17
Experiment ID
208
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
18
Experiment ID
209
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
19
Experiment ID
1203
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
34887515
Organism
Homo sapiens
Experiment description
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Colorectal cancer cells
Sample name
DiFi
Isolation/purification methods
Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting Mass spectrometry RNA sequencing
20
Experiment ID
407
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
21
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
22
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
23
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
24
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
25
Experiment ID
405
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Foreskin fibroblasts
Sample name
BJ
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
26
Experiment ID
363
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
27
Experiment ID
364
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
28
Experiment ID
365
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
29
Experiment ID
189
MISEV standards
✔
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19530224
Organism
Homo sapiens
Experiment description
Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors
"Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name
J Cell Physiol
Publication year
2009
Sample
Keratinocytes
Sample name
Keratinocytes - Differentiated
Isolation/purification methods
Differential centrifugation Filtration Sucrose cushion Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
30
Experiment ID
190
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19530224
Organism
Homo sapiens
Experiment description
Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors
"Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name
J Cell Physiol
Publication year
2009
Sample
Keratinocytes
Sample name
Keratinocytes - Undifferentiated
Isolation/purification methods
Differential centrifugation Filtration Sucrose cushion Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
31
Experiment ID
417
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Lymphoma cells
Sample name
Raji
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
32
Experiment ID
411
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Mammary cancer-associated fibroblasts
Sample name
mCAF
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
33
Experiment ID
126
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [LTQ-FT Ultra] Western blotting
PubMed ID
Unpublished / Not applicable
Organism
Homo sapiens
Experiment description
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors
"Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name
Mesenchymal Stem Cell Therapy
Publication year
2011
Sample
Mesenchymal stem cells
Sample name
huES9.E1
Isolation/purification methods
HPLC
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Antobody array Mass spectrometry
34
Experiment ID
488
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Homo sapiens
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Mesenchymal stem cells
Sample name
UCMSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
35
Experiment ID
418
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
36
Experiment ID
224
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
37
Experiment ID
413
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Normal mammary epithelial cells
Sample name
MCF10A
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
38
Experiment ID
211
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
39
Experiment ID
212
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
40
Experiment ID
406
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
BxPC3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
41
Experiment ID
415
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
42
Experiment ID
434
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
43
Experiment ID
435
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
44
Experiment ID
408
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPDE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
45
Experiment ID
409
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPNE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
46
Experiment ID
231
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
47
Experiment ID
232
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
48
Experiment ID
233
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
49
Experiment ID
416
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pluripotent stem cells
Sample name
PSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
50
Experiment ID
138
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
DU145 - Rep 2
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
51
Experiment ID
139
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
DU145 - Rep 3
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
52
Experiment ID
140
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
VCaP - Rep 2
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
53
Experiment ID
141
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
VCaP - Rep 3
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
54
Experiment ID
142
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
LNCaP - Rep 2
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
55
Experiment ID
145
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
C4-2 - Rep 3
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
56
Experiment ID
191
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20124223
Organism
Homo sapiens
Experiment description
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors
"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name
Mol Cell Proteomics
Publication year
2010
Sample
Squamous carcinoma cells
Sample name
Squamous carcinoma cell (A431)
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
57
Experiment ID
410
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
T lymphocytes
Sample name
Jurkat
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
58
Experiment ID
217
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for PSMA6
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
UBE2H
7328
Affinity Capture-MS
Homo sapiens
2
PSMA6
5687
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
3
LIG4
3981
Two-hybrid
Homo sapiens
4
PPP3CA
5530
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
5
CCT3
7203
Co-fractionation
Homo sapiens
6
PAXIP1
Two-hybrid
Homo sapiens
7
Psmb5
19173
Affinity Capture-MS
Mus musculus
8
PSMD6
9861
Affinity Capture-Western
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
9
ARHGAP39
Affinity Capture-MS
Homo sapiens
10
PSMA4
5685
Affinity Capture-Western
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
11
RIT1
6016
Negative Genetic
Homo sapiens
12
RACGAP1
29127
Affinity Capture-MS
Homo sapiens
13
TARDBP
23435
Co-fractionation
Homo sapiens
14
Skp1a
21402
Affinity Capture-MS
Mus musculus
15
UCHL1
7345
Affinity Capture-Western
Homo sapiens
16
DNAJB2
3300
Affinity Capture-Western
Homo sapiens
17
SOX2
Affinity Capture-MS
Homo sapiens
18
BRCA1
672
Two-hybrid
Homo sapiens
19
CAPZA1
829
Co-fractionation
Homo sapiens
20
UBE3A
7337
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
21
TXNL1
9352
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
22
UBC
7316
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
23
CCT4
10575
Co-fractionation
Homo sapiens
24
PSMD11
5717
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
25
MID1
4281
Affinity Capture-MS
Homo sapiens
26
SYVN1
84447
Affinity Capture-MS
Homo sapiens
27
ISG15
9636
Affinity Capture-MS
Homo sapiens
28
PSMB8
5696
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
29
RPS3
6188
Co-fractionation
Homo sapiens
30
KIAA1429
25962
Affinity Capture-MS
Homo sapiens
31
RNH1
6050
Co-fractionation
Homo sapiens
32
UBD
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
33
TOP2A
7153
Affinity Capture-MS
Homo sapiens
34
PSMG3
84262
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
35
PGK1
5230
Co-fractionation
Homo sapiens
36
B3GNT2
10678
Affinity Capture-MS
Homo sapiens
37
MRPL40
64976
Cross-Linking-MS (XL-MS)
Homo sapiens
38
KLF8
Affinity Capture-MS
Homo sapiens
39
SNRPA1
6627
Co-fractionation
Homo sapiens
40
CCNF
Affinity Capture-Western
Homo sapiens
41
SMCO3
Two-hybrid
Homo sapiens
42
COPS5
10987
Affinity Capture-Western
Homo sapiens
43
PSMD9
5715
Co-fractionation
Homo sapiens
44
PSMD12
5718
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
45
PSMB6
5694
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
46
PSMC6
5706
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
47
PSMC2
5701
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
48
UBQLN1
29979
Affinity Capture-Western
Homo sapiens
49
HSP90B1
7184
Co-fractionation
Homo sapiens
50
KRAS
3845
Negative Genetic
Homo sapiens
51
PYCRL
65263
Co-fractionation
Homo sapiens
52
EXOSC4
54512
Co-fractionation
Homo sapiens
53
ITGA4
3676
Affinity Capture-MS
Homo sapiens
54
PSMG1
8624
Affinity Capture-Western
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
55
UCHL5
51377
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
56
PSMB9
5698
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
57
VCAM1
7412
Affinity Capture-MS
Homo sapiens
58
PSMA8
143471
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
59
FBXO7
25793
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
60
PSMD5
5711
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
61
CALR
811
Co-fractionation
Homo sapiens
62
KRTAP4-2
Two-hybrid
Homo sapiens
63
CUL1
8454
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
64
ELP6
54859
Co-fractionation
Homo sapiens
65
GPS1
2873
Affinity Capture-Western
Homo sapiens
66
USP53
54532
Affinity Capture-MS
Homo sapiens
67
SOD1
6647
Affinity Capture-MS
Homo sapiens
68
PSMC3
5702
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
69
RAD23A
5886
Affinity Capture-MS
Homo sapiens
70
LIMD2
Two-hybrid
Homo sapiens
71
PRNP
5621
Affinity Capture-MS
Homo sapiens
72
EXOSC9
5393
Co-fractionation
Homo sapiens
73
PSME3
10197
Co-fractionation
Homo sapiens
74
TRIM39
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
75
CHMP4C
92421
Affinity Capture-MS
Homo sapiens
76
AURKA
6790
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
77
PSMB4
5692
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
78
RBX1
9978
Affinity Capture-Western
Homo sapiens
79
USP14
9097
Affinity Capture-MS
Homo sapiens
80
PSMD4
5710
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
81
RNF170
81790
Two-hybrid
Homo sapiens
82
RB1CC1
9821
Affinity Capture-MS
Homo sapiens
83
WDR11
55717
Co-fractionation
Homo sapiens
84
AMFR
267
Affinity Capture-MS
Homo sapiens
85
PSME1
5720
Affinity Capture-Western
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
86
TBR1
Proximity Label-MS
Homo sapiens
87
C19orf47
Two-hybrid
Homo sapiens
88
PSMC1
5700
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
89
PSMD14
10213
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
90
CAST
831
Co-fractionation
Homo sapiens
91
PSMG2
56984
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
92
CCNB1
891
Affinity Capture-Western
Homo sapiens
93
RANBP1
5902
Affinity Capture-MS
Homo sapiens
94
FAM192A
Affinity Capture-MS
Homo sapiens
95
NELFE
7936
Co-fractionation
Homo sapiens
96
PSMD2
5708
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
97
VCP
7415
Affinity Capture-Western
Homo sapiens
Co-fractionation
Homo sapiens
98
ACTN4
81
Co-fractionation
Homo sapiens
99
NTRK1
4914
Affinity Capture-MS
Homo sapiens
100
PSMD8
5714
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
101
EXOSC5
56915
Co-fractionation
Homo sapiens
102
PLK1
5347
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
103
ZKSCAN4
Two-hybrid
Homo sapiens
104
NOS2
Affinity Capture-MS
Homo sapiens
105
COG3
83548
Affinity Capture-MS
Homo sapiens
106
CAND2
23066
Co-fractionation
Homo sapiens
107
RTP5
Two-hybrid
Homo sapiens
108
CEBPA
Protein-peptide
Homo sapiens
109
CYLD
Affinity Capture-MS
Homo sapiens
110
MBL2
4153
Affinity Capture-MS
Homo sapiens
111
ZBTB44
Two-hybrid
Homo sapiens
112
BAG3
9531
Affinity Capture-MS
Homo sapiens
113
ATG16L1
55054
Affinity Capture-MS
Homo sapiens
114
ANKZF1
55139
Affinity Capture-MS
Homo sapiens
115
KIF1B
23095
Co-fractionation
Homo sapiens
116
Inpp5b
Affinity Capture-MS
Mus musculus
117
PSMA3
5684
Co-purification
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-fractionation
Homo sapiens
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-fractionation
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
118
CCNA2
890
Affinity Capture-Western
Homo sapiens
119
COPS2
9318
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
120
USP4
7375
Two-hybrid
Homo sapiens
121
C19orf57
Two-hybrid
Homo sapiens
122
PREX1
Affinity Capture-MS
Homo sapiens
123
CANX
821
Co-fractionation
Homo sapiens
124
AKIRIN1
Affinity Capture-MS
Homo sapiens
125
EXOSC6
118460
Co-fractionation
Homo sapiens
126
AMBRA1
Affinity Capture-MS
Homo sapiens
127
BUB1B
Affinity Capture-MS
Homo sapiens
128
ECT2
1894
Two-hybrid
Homo sapiens
129
PSMD13
5719
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
130
TAL1
Affinity Capture-MS
Homo sapiens
131
UBQLN2
29978
Affinity Capture-Western
Homo sapiens
132
IQCB1
Affinity Capture-MS
Homo sapiens
133
SIRPA
140885
Reconstituted Complex
Homo sapiens
134
SLX1B
Affinity Capture-MS
Homo sapiens
135
CCNE1
Affinity Capture-Western
Homo sapiens
136
PSMA2
5683
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Co-purification
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
137
FKBP8
23770
Affinity Capture-MS
Homo sapiens
138
RPP30
10556
Co-fractionation
Homo sapiens
139
PSMD7
5713
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
140
EEF1A1
1915
Co-fractionation
Homo sapiens
141
FN1
2335
Affinity Capture-MS
Homo sapiens
142
XBP1
Affinity Capture-Western
Homo sapiens
143
SF3A1
10291
Co-fractionation
Homo sapiens
144
CDK2
1017
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
145
XPOT
11260
Co-fractionation
Homo sapiens
146
PSMD10
5716
Co-fractionation
Homo sapiens
147
SERBP1
26135
Affinity Capture-MS
Homo sapiens
148
UNKL
Affinity Capture-MS
Homo sapiens
149
PSMC5
5705
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
150
PSMB5
5693
Co-fractionation
Homo sapiens
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
151
MFN2
9927
Proximity Label-MS
Homo sapiens
152
DDRGK1
65992
Affinity Capture-MS
Homo sapiens
153
RNF2
Affinity Capture-MS
Homo sapiens
154
KIAA0368
23392
Co-localization
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
155
RNF185
Affinity Capture-MS
Homo sapiens
156
CCND1
595
Affinity Capture-Western
Homo sapiens
157
ADRM1
11047
Co-fractionation
Homo sapiens
158
ENTHD2
Two-hybrid
Homo sapiens
159
POLR2A
5430
Co-fractionation
Homo sapiens
160
PSMB1
5689
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
161
TPM4
7171
Co-fractionation
Homo sapiens
162
HUWE1
10075
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
163
ISY1-RAB43
100534599
Co-fractionation
Homo sapiens
164
TPM3
7170
Co-fractionation
Homo sapiens
165
PSMB3
5691
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
166
ISY1
57461
Co-fractionation
Homo sapiens
167
PSMB2
5690
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
168
RPS27A
6233
Affinity Capture-MS
Homo sapiens
169
PLEKHA4
57664
Affinity Capture-MS
Homo sapiens
170
SPOP
Affinity Capture-MS
Homo sapiens
171
CCT8
10694
Co-fractionation
Homo sapiens
172
PARP1
142
Proximity Label-MS
Homo sapiens
173
CADM1
23705
Two-hybrid
Homo sapiens
174
UBXN4
23190
Affinity Capture-Western
Homo sapiens
175
Psmb4
19172
Affinity Capture-MS
Mus musculus
176
ERH
2079
Two-hybrid
Homo sapiens
177
POLR2L
5441
Two-hybrid
Homo sapiens
178
DNAJA2
10294
Co-fractionation
Homo sapiens
179
ZNF596
Two-hybrid
Homo sapiens
180
PSMB7
5695
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
181
HNRNPA2B1
3181
Co-fractionation
Homo sapiens
182
PSME2
5721
Co-fractionation
Homo sapiens
183
POMP
Co-fractionation
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
184
UBA52
7311
Affinity Capture-MS
Homo sapiens
185
SQSTM1
8878
Affinity Capture-Western
Homo sapiens
186
NHLRC2
374354
Affinity Capture-MS
Homo sapiens
187
SHFM1
7979
Affinity Capture-MS
Homo sapiens
188
HSPB1
3315
Two-hybrid
Homo sapiens
189
TSPYL1
7259
Two-hybrid
Homo sapiens
190
CDK1
983
Affinity Capture-Western
Homo sapiens
191
PSMA1
5682
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
192
UBE3C
9690
Affinity Capture-MS
Homo sapiens
193
UFL1
23376
Affinity Capture-MS
Homo sapiens
194
PSMA5
5686
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
195
CDK4
1019
Affinity Capture-Western
Homo sapiens
196
PSMA7
5688
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
197
ATP5B
506
Co-fractionation
Homo sapiens
198
CCT5
22948
Co-fractionation
Homo sapiens
199
PSMF1
9491
Affinity Capture-MS
Homo sapiens
200
MAF
Affinity Capture-MS
Homo sapiens
201
CEP85
Affinity Capture-MS
Homo sapiens
202
COX7C
1350
Co-fractionation
Homo sapiens
203
C9orf72
Affinity Capture-MS
Homo sapiens
204
AR
367
Affinity Capture-MS
Homo sapiens
205
RNF113A
7737
Co-fractionation
Homo sapiens
206
PSMD1
5707
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
207
ATXN3
4287
Affinity Capture-MS
Homo sapiens
208
PSMD3
5709
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
209
PSMC4
5704
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
210
PTCH1
Affinity Capture-MS
Homo sapiens
211
PRMT1
3276
Affinity Capture-MS
Homo sapiens
212
RBCK1
Affinity Capture-MS
Homo sapiens
213
RPP38
10557
Co-fractionation
Homo sapiens
214
NEDD8
4738
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
215
DERL1
79139
Affinity Capture-MS
Homo sapiens
216
PSME4
23198
Affinity Capture-MS
Homo sapiens
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Pathways in which PSMA6 is involved