Gene description for ANKZF1
Gene name ankyrin repeat and zinc finger domain containing 1
Gene symbol ANKZF1
Other names/aliases ZNF744
Species Homo sapiens
 Database cross references - ANKZF1
ExoCarta ExoCarta_55139
Vesiclepedia VP_55139
Entrez Gene 55139
HGNC 25527
UniProt Q9H8Y5  
 ANKZF1 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ANKZF1
Molecular Function
    RNA endonuclease activity GO:0004521 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    catalytic activity, acting on a tRNA GO:0140101 IDA
Biological Process
    protein quality control for misfolded or incompletely synthesized proteins GO:0006515 IDA
    ERAD pathway GO:0036503 IBA
    cellular response to hydrogen peroxide GO:0070301 IMP
    rescue of stalled ribosome GO:0072344 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    membrane GO:0016020 HDA
 Experiment description of studies that identified ANKZF1 in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ANKZF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMA6 5687
Affinity Capture-MS Homo sapiens
2 PSMB2 5690
Affinity Capture-MS Homo sapiens
3 STAT6 6778
Two-hybrid Homo sapiens
4 PSMC3 5702
Affinity Capture-MS Homo sapiens
5 PSMD6 9861
Affinity Capture-MS Homo sapiens
6 FAS 355
Proximity Label-MS Homo sapiens
7 EGLN3  
Affinity Capture-MS Homo sapiens
8 RC3H2  
Affinity Capture-MS Homo sapiens
9 PSMD11 5717
Affinity Capture-MS Homo sapiens
10 YWHAE 7531
Reconstituted Complex Homo sapiens
11 PSMB7 5695
Affinity Capture-MS Homo sapiens
12 UBL4B  
Affinity Capture-MS Homo sapiens
13 EDEM1  
Affinity Capture-MS Homo sapiens
14 APRT 353
Affinity Capture-MS Homo sapiens
15 PARK2  
Affinity Capture-MS Homo sapiens
16 PSMD7 5713
Affinity Capture-MS Homo sapiens
17 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
18 PSMD13 5719
Affinity Capture-MS Homo sapiens
19 XPO1 7514
Affinity Capture-MS Homo sapiens
20 PSMC5 5705
Affinity Capture-MS Homo sapiens
21 NAA35 60560
Co-fractionation Homo sapiens
22 PSMA5 5686
Affinity Capture-MS Homo sapiens
23 VCP 7415
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 FBXW7  
Affinity Capture-MS Homo sapiens
25 PSMD12 5718
Affinity Capture-MS Homo sapiens
26 PSMC6 5706
Affinity Capture-MS Homo sapiens
27 PSMC4 5704
Affinity Capture-MS Homo sapiens
28 DNAJB11 51726
Two-hybrid Homo sapiens
29 PSMD3 5709
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ANKZF1 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here