Gene description for CADM1
Gene name cell adhesion molecule 1
Gene symbol CADM1
Other names/aliases BL2
IGSF4
IGSF4A
NECL2
Necl-2
RA175
ST17
SYNCAM
TSLC1
sTSLC-1
sgIGSF
synCAM1
Species Homo sapiens
 Database cross references - CADM1
ExoCarta ExoCarta_23705
Vesiclepedia VP_23705
Entrez Gene 23705
HGNC 5951
MIM 605686
UniProt Q9BY67  
 CADM1 identified in sEVs derived from the following tissue/cell type
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CADM1
Molecular Function
    signaling receptor binding GO:0005102 IBA
    signaling receptor binding GO:0005102 IPI
    signaling receptor binding GO:0005102 ISS
    protein binding GO:0005515 IPI
    PDZ domain binding GO:0030165 ISS
    protein homodimerization activity GO:0042803 ISS
    cell adhesion molecule binding GO:0050839 IEA
Biological Process
    positive regulation of cytokine production GO:0001819 IDA
    liver development GO:0001889 IEA
    T cell mediated cytotoxicity GO:0001913 IDA
    apoptotic process GO:0006915 IEA
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IBA
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 ISS
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 ISS
    spermatogenesis GO:0007283 IEA
    cell recognition GO:0008037 IBA
    cell recognition GO:0008037 IDA
    cell recognition GO:0008037 ISS
    cell differentiation GO:0030154 IEA
    susceptibility to natural killer cell mediated cytotoxicity GO:0042271 IBA
    susceptibility to natural killer cell mediated cytotoxicity GO:0042271 IDA
    susceptibility to natural killer cell mediated cytotoxicity GO:0042271 ISS
    positive regulation of natural killer cell mediated cytotoxicity GO:0045954 IDA
    positive regulation of natural killer cell mediated cytotoxicity GO:0045954 ISS
    activated T cell proliferation GO:0050798 IDA
    detection of stimulus GO:0051606 IBA
    detection of stimulus GO:0051606 IDA
    detection of stimulus GO:0051606 ISS
    negative regulation of ERBB4 signaling pathway GO:0120154 IEA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 ISS
    postsynaptic density GO:0014069 IEA
    basolateral plasma membrane GO:0016323 ISS
    dendrite GO:0030425 IEA
    neuron projection GO:0043005 IBA
    synapse GO:0045202 IBA
 Experiment description of studies that identified CADM1 in sEVs
1
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CADM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMA6 5687
Two-hybrid Homo sapiens
2 CNBP 7555
Co-fractionation Homo sapiens
3 PTPN4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LGALS3 3958
Affinity Capture-MS Homo sapiens
5 KRAS 3845
Proximity Label-MS Homo sapiens
6 EPB41L3 23136
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 NR3C1 2908
Proximity Label-MS Homo sapiens
8 RPS15 6209
Co-fractionation Homo sapiens
9 RPA3 6119
Proximity Label-MS Homo sapiens
10 MPP3  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 CTDSPL 10217
Proximity Label-MS Homo sapiens
12 ARF6 382
Proximity Label-MS Homo sapiens
13 SPPL2B 56928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 LIN7A 8825
Affinity Capture-MS Homo sapiens
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