Gene description for Psmb4
Gene name proteasome (prosome, macropain) subunit, beta type 4
Gene symbol Psmb4
Other names/aliases Pros-27
Species Mus musculus
 Database cross references - Psmb4
ExoCarta ExoCarta_19172
Vesiclepedia VP_19172
Entrez Gene 19172
UniProt P99026  
 Psmb4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Neural stem cells 25242146    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Psmb4
Molecular Function
    lipopolysaccharide binding GO:0001530 IDA
Biological Process
    negative regulation of inflammatory response to antigenic stimulus GO:0002862 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteolysis involved in protein catabolic process GO:0051603 IEA
Subcellular Localization
    proteasome complex GO:0000502 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    proteasome core complex GO:0005839 IDA
    proteasome core complex GO:0005839 ISO
    proteasome core complex, beta-subunit complex GO:0019774 IBA
    proteasome core complex, beta-subunit complex GO:0019774 ISS
    ciliary basal body GO:0036064 IEA
 Experiment description of studies that identified Psmb4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Psmb4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMA6 5687
Affinity Capture-MS Homo sapiens
2 PSMB2 5690
Affinity Capture-MS Homo sapiens
3 PSME2 5721
Affinity Capture-MS Homo sapiens
4 FNBP4  
Affinity Capture-MS Homo sapiens
5 PSMA3 5684
Affinity Capture-MS Homo sapiens
6 Psmd2 21762
Co-fractionation Mus musculus
7 Psmd8 57296
Co-fractionation Mus musculus
8 PSMC3 5702
Affinity Capture-MS Homo sapiens
9 FBXO7 25793
Affinity Capture-MS Homo sapiens
10 PSMA4 5685
Affinity Capture-MS Homo sapiens
11 FAM192A  
Affinity Capture-MS Homo sapiens
12 Psmd13 23997
Co-fractionation Mus musculus
13 PSMB7 5695
Affinity Capture-MS Homo sapiens
14 AKIRIN2  
Affinity Capture-MS Homo sapiens
15 HPS5 11234
Affinity Capture-MS Homo sapiens
16 CDK18 5129
Affinity Capture-MS Homo sapiens
17 Uchl5 56207
Co-fractionation Mus musculus
18 PSMA2 5683
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
19 Vcp 269523
Co-fractionation Mus musculus
20 PSMD4 5710
Affinity Capture-MS Homo sapiens
21 Psmd14 59029
Affinity Capture-Western Mus musculus
22 Iqcb1  
Affinity Capture-MS Mus musculus
23 PSME1 5720
Affinity Capture-MS Homo sapiens
24 Psmd9  
Co-fractionation Mus musculus
25 PSMC1 5700
Affinity Capture-MS Homo sapiens
26 PSMA1 5682
Affinity Capture-MS Homo sapiens
27 PSMD14 10213
Affinity Capture-MS Homo sapiens
28 PSMG2 56984
Affinity Capture-MS Homo sapiens
29 SMARCA4 6597
Affinity Capture-MS Homo sapiens
30 DROSHA  
Affinity Capture-MS Homo sapiens
31 ATP9A 10079
Affinity Capture-MS Homo sapiens
32 PSMA5 5686
Affinity Capture-MS Homo sapiens
33 PSMA7 5688
Affinity Capture-MS Homo sapiens
34 TMEM109 79073
Affinity Capture-MS Homo sapiens
35 DNAJC8 22826
Affinity Capture-MS Homo sapiens
36 SPATA5 166378
Affinity Capture-MS Homo sapiens
37 PSMC5 5705
Affinity Capture-MS Homo sapiens
38 PSMB5 5693
Affinity Capture-MS Homo sapiens
39 ADRM1 11047
Affinity Capture-MS Homo sapiens
40 PSMB6 5694
Affinity Capture-MS Homo sapiens
41 PSMC6 5706
Affinity Capture-MS Homo sapiens
42 PSMD1 5707
Affinity Capture-MS Homo sapiens
43 PSME3 10197
Affinity Capture-MS Homo sapiens
44 PSMG1 8624
Affinity Capture-MS Homo sapiens
45 CEP120  
Affinity Capture-MS Homo sapiens
46 PSMC2 5701
Affinity Capture-MS Homo sapiens
47 Psmd6 66413
Co-fractionation Mus musculus
48 PSMB1 5689
Affinity Capture-MS Homo sapiens
49 PSMD3 5709
Affinity Capture-MS Homo sapiens
50 PSMB3 5691
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Psmb4 is involved
PathwayEvidenceSource
ABC-family proteins mediated transport IEA Reactome
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
Activation of NF-kappaB in B cells IEA Reactome
Adaptive Immune System IEA Reactome
Antigen processing-Cross presentation IEA Reactome
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
APC/C-mediated degradation of cell cycle proteins IEA Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins IEA Reactome
APC/C:Cdc20 mediated degradation of Securin IEA Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 IEA Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint IEA Reactome
Assembly of the pre-replicative complex IEA Reactome
Asymmetric localization of PCP proteins IEA Reactome
AUF1 (hnRNP D0) binds and destabilizes mRNA IEA Reactome
Autodegradation of Cdh1 by Cdh1:APC/C IEA Reactome
Autodegradation of the E3 ubiquitin ligase COP1 IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A IEA Reactome
CDK-mediated phosphorylation and removal of Cdc6 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to chemical stress IEA Reactome
Cellular response to hypoxia IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Class I MHC mediated antigen processing & presentation IEA Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
Cross-presentation of soluble exogenous antigens (endosomes) IEA Reactome
Cyclin A:Cdk2-associated events at S phase entry IEA Reactome
Cyclin E associated events during G1/S transition IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Dectin-1 mediated noncanonical NF-kB signaling IEA Reactome
Degradation of AXIN IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
Degradation of DVL IEA Reactome
Degradation of GLI1 by the proteasome IEA Reactome
Deubiquitination IEA Reactome
DNA Replication IEA Reactome
DNA Replication Pre-Initiation IEA Reactome
Downstream signaling events of B Cell Receptor (BCR) IEA Reactome
Downstream TCR signaling IEA Reactome
FBXL7 down-regulates AURKA during mitotic entry and in early mitosis IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated NF-kB activation IEA Reactome
G1/S DNA Damage Checkpoints IEA Reactome
G1/S Transition IEA Reactome
G2/M Checkpoints IEA Reactome
G2/M Transition IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
GLI3 is processed to GLI3R by the proteasome IEA Reactome
GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 IEA Reactome
Hedgehog 'off' state IEA Reactome
Hedgehog 'on' state IEA Reactome
Hedgehog ligand biogenesis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Interleukin-1 family signaling IEA Reactome
Interleukin-1 signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
KEAP1-NFE2L2 pathway IEA Reactome
M Phase IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
MAPK6/MAPK4 signaling IEA Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome
Metabolism of polyamines IEA Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G1 phase and G1/S transition IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Neddylation IEA Reactome
NIK-->noncanonical NF-kB signaling IEA Reactome
Nuclear events mediated by NFE2L2 IEA Reactome
Orc1 removal from chromatin IEA Reactome
Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha IEA Reactome
p53-Dependent G1 DNA Damage Response IEA Reactome
p53-Dependent G1/S DNA damage checkpoint IEA Reactome
p53-Independent DNA Damage Response IEA Reactome
p53-Independent G1/S DNA damage checkpoint IEA Reactome
PCP/CE pathway IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Post-translational protein modification IEA Reactome
Proteasome assembly IEA Reactome
PTEN Regulation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Regulation of mitotic cell cycle IEA Reactome
Regulation of mRNA stability by proteins that bind AU-rich elements IEA Reactome
Regulation of ornithine decarboxylase (ODC) IEA Reactome
Regulation of PTEN stability and activity IEA Reactome
Regulation of RAS by GAPs IEA Reactome
Regulation of RUNX2 expression and activity IEA Reactome
Regulation of RUNX3 expression and activity IEA Reactome
RNA Polymerase II Transcription IEA Reactome
RUNX1 regulates transcription of genes involved in differentiation of HSCs IEA Reactome
S Phase IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Signaling by WNT IEA Reactome
Stabilization of p53 IEA Reactome
Switching of origins to a post-replicative state IEA Reactome
Synthesis of DNA IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
TCR signaling IEA Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint IEA Reactome
TNFR2 non-canonical NF-kB pathway IEA Reactome
Transcriptional regulation by RUNX1 IEA Reactome
Transcriptional regulation by RUNX2 IEA Reactome
Transcriptional regulation by RUNX3 IEA Reactome
Transport of small molecules IEA Reactome
Ub-specific processing proteases IEA Reactome
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A IEA Reactome
Ubiquitin-dependent degradation of Cyclin D IEA Reactome
UCH proteinases IEA Reactome





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