Gene description for DNAJC8
Gene name DnaJ (Hsp40) homolog, subfamily C, member 8
Gene symbol DNAJC8
Other names/aliases HSPC331
SPF31
Species Homo sapiens
 Database cross references - DNAJC8
ExoCarta ExoCarta_22826
Vesiclepedia VP_22826
Entrez Gene 22826
HGNC 15470
UniProt O75937  
 DNAJC8 identified in sEVs derived from the following tissue/cell type
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
 Gene ontology annotations for DNAJC8
Molecular Function
    protein binding GO:0005515 IPI
    Hsp70 protein binding GO:0030544 IPI
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IDA
    intercellular bridge GO:0045171 IDA
 Experiment description of studies that identified DNAJC8 in sEVs
1
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
2
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
3
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
11
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
12
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
13
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
14
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
16
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
20
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
24
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for DNAJC8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Proximity Label-MS Homo sapiens
2 CD3EAP  
Proximity Label-MS Homo sapiens
3 RALY 22913
Proximity Label-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 TCEB1 6921
Affinity Capture-MS Homo sapiens
6 COIL  
Proximity Label-MS Homo sapiens
7 RPS3A 6189
Affinity Capture-MS Homo sapiens
8 RPL30 6156
Proximity Label-MS Homo sapiens
9 YTHDF1 54915
Affinity Capture-MS Homo sapiens
10 SRPK2 6733
Biochemical Activity Homo sapiens
11 RCL1 10171
Proximity Label-MS Homo sapiens
12 FBXO25  
Biochemical Activity Homo sapiens
13 ACTC1 70
Proximity Label-MS Homo sapiens
14 NOP56 10528
Proximity Label-MS Homo sapiens
15 HSPA1L 3305
Affinity Capture-MS Homo sapiens
16 SURF4 6836
Proximity Label-MS Homo sapiens
17 ODF2 4957
Cross-Linking-MS (XL-MS) Homo sapiens
18 BRD2  
Proximity Label-MS Homo sapiens
19 ROCK2 9475
Affinity Capture-MS Homo sapiens
20 PHF5A 84844
Co-fractionation Homo sapiens
21 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
22 RRP12 23223
Proximity Label-MS Homo sapiens
23 SNRPF 6636
Affinity Capture-MS Homo sapiens
24 SNRPD2 6633
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
25 APP 351
Reconstituted Complex Homo sapiens
26 SF3B5 83443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SF3B2 10992
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
28 APEX1 328
Proximity Label-MS Homo sapiens
29 NOL8  
Proximity Label-MS Homo sapiens
30 HECW2  
Affinity Capture-MS Homo sapiens
31 PMPCA 23203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 EGR1  
Affinity Capture-MS Homo sapiens
33 H1FX 8971
Affinity Capture-MS Homo sapiens
34 SF3B1 23451
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 HDLBP 3069
Affinity Capture-MS Homo sapiens
36 H2AFV 94239
Cross-Linking-MS (XL-MS) Homo sapiens
37 E2F6 1876
Proximity Label-MS Homo sapiens
38 SRRM2 23524
Affinity Capture-MS Homo sapiens
39 ZNF512B  
Proximity Label-MS Homo sapiens
40 RECQL4  
Affinity Capture-MS Homo sapiens
41 ATAD5  
Proximity Label-MS Homo sapiens
42 SF3A2 8175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 DNAJB1 3337
Affinity Capture-MS Homo sapiens
44 SRP14 6727
Proximity Label-MS Homo sapiens
45 AGTR1  
Two-hybrid Homo sapiens
46 RPF2 84154
Proximity Label-MS Homo sapiens
47 HSPH1 10808
Affinity Capture-MS Homo sapiens
48 WDR3  
Proximity Label-MS Homo sapiens
49 SMEK1 55671
Affinity Capture-MS Homo sapiens
50 ATRX 546
Proximity Label-MS Homo sapiens
51 XRCC3  
Affinity Capture-MS Homo sapiens
52 NUCB1 4924
Affinity Capture-MS Homo sapiens
53 CST1  
Affinity Capture-MS Homo sapiens
54 SLC4A1AP  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 MYCN  
Affinity Capture-MS Homo sapiens
56 PPP3R1 5534
Affinity Capture-MS Homo sapiens
57 SREK1 140890
Proximity Label-MS Homo sapiens
58 PES1 23481
Proximity Label-MS Homo sapiens
59 METTL13 51603
Co-fractionation Homo sapiens
60 E2F4  
Affinity Capture-MS Homo sapiens
61 RPS23 6228
Affinity Capture-MS Homo sapiens
62 P4HA1 5033
Affinity Capture-MS Homo sapiens
63 PPP5C 5536
Affinity Capture-MS Homo sapiens
64 TOP2B 7155
Proximity Label-MS Homo sapiens
65 RPL4 6124
Affinity Capture-MS Homo sapiens
66 RBM28 55131
Proximity Label-MS Homo sapiens
67 FAM204A  
Affinity Capture-MS Homo sapiens
68 RRP9 9136
Proximity Label-MS Homo sapiens
69 BTF3L4 91408
Affinity Capture-MS Homo sapiens
70 HELLS 3070
Proximity Label-MS Homo sapiens
71 WBP11  
Proximity Label-MS Homo sapiens
72 SRSF6 6431
Affinity Capture-MS Homo sapiens
73 CBX3 11335
Proximity Label-MS Homo sapiens
74 SNRPB 6628
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
75 DDX10  
Proximity Label-MS Homo sapiens
76 PMAIP1  
Affinity Capture-MS Homo sapiens
77 CBX5 23468
Proximity Label-MS Homo sapiens
78 NOC4L 79050
Proximity Label-MS Homo sapiens
79 U2AF2 11338
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
80 RAD18  
Affinity Capture-MS Homo sapiens
81 RAP1A 5906
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
82 SF3B6 51639
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
84 UBTF 7343
Proximity Label-MS Homo sapiens
85 EIF4G2 1982
Affinity Capture-MS Homo sapiens
86 DHX9 1660
Affinity Capture-MS Homo sapiens
87 CTR9 9646
Proximity Label-MS Homo sapiens
88 RPLP0 6175
Affinity Capture-MS Homo sapiens
89 EED  
Proximity Label-MS Homo sapiens
90 SND1 27044
Affinity Capture-MS Homo sapiens
91 H2AFY 9555
Proximity Label-MS Homo sapiens
92 PPM1G 5496
Affinity Capture-MS Homo sapiens
93 CEBPA  
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
94 SRPK1 6732
Biochemical Activity Homo sapiens
95 GPATCH11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 SNRPB2 6629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 GTF2A1 2957
Proximity Label-MS Homo sapiens
98 REEP5 7905
Affinity Capture-MS Homo sapiens
99 TPX2  
Co-fractionation Homo sapiens
100 GDI2 2665
Affinity Capture-MS Homo sapiens
101 DNAJB11 51726
Co-fractionation Homo sapiens
102 UBE2N 7334
Affinity Capture-MS Homo sapiens
103 HSPA5 3309
Co-fractionation Homo sapiens
104 DDX46 9879
Co-fractionation Homo sapiens
105 RBM39 9584
Affinity Capture-MS Homo sapiens
106 BMS1  
Proximity Label-MS Homo sapiens
107 CUL5 8065
Co-fractionation Homo sapiens
108 PPP4R2  
Affinity Capture-MS Homo sapiens
109 SMARCAD1  
Proximity Label-MS Homo sapiens
110 POP1 10940
Proximity Label-MS Homo sapiens
111 EIF6 3692
Affinity Capture-MS Homo sapiens
112 Kifc1  
Affinity Capture-MS Mus musculus
113 HMOX2 3163
Cross-Linking-MS (XL-MS) Homo sapiens
114 LAMP1 3916
Affinity Capture-MS Homo sapiens
115 RPS8 6202
Affinity Capture-MS Homo sapiens
116 C1orf52  
Proximity Label-MS Homo sapiens
117 SLK 9748
Affinity Capture-MS Homo sapiens
118 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
119 LRRC47 57470
Affinity Capture-MS Homo sapiens
120 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
121 FUS 2521
Affinity Capture-MS Homo sapiens
122 RPL7 6129
Affinity Capture-MS Homo sapiens
123 WIBG 84305
Affinity Capture-MS Homo sapiens
124 RBBP4 5928
Affinity Capture-MS Homo sapiens
125 NIP7 51388
Affinity Capture-MS Homo sapiens
126 PUS7 54517
Affinity Capture-MS Homo sapiens
127 PKN2 5586
Affinity Capture-MS Homo sapiens
128 SF3A1 10291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
129 ZNF512  
Proximity Label-MS Homo sapiens
130 PMPCB 9512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 CHD1 1105
Proximity Label-MS Homo sapiens
132 DDX42 11325
Affinity Capture-MS Homo sapiens
133 MFAP1  
Proximity Label-MS Homo sapiens
134 RPL17 6139
Affinity Capture-MS Homo sapiens
135 MYC  
Affinity Capture-MS Homo sapiens
136 BAG1 573
Affinity Capture-MS Homo sapiens
137 LTV1  
Affinity Capture-MS Homo sapiens
138 CNTROB  
Affinity Capture-MS Homo sapiens
139 EMC2 9694
Affinity Capture-MS Homo sapiens
140 SARNP 84324
Cross-Linking-MS (XL-MS) Homo sapiens
141 RPL35A 6165
Proximity Label-MS Homo sapiens
142 EHMT2 10919
Proximity Label-MS Homo sapiens
143 TTC9C 283237
Affinity Capture-MS Homo sapiens
144 WDR75  
Proximity Label-MS Homo sapiens
145 HYOU1 10525
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
146 Junb  
Affinity Capture-MS Mus musculus
147 SF3B3 23450
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
148 KRAS 3845
Negative Genetic Homo sapiens
149 HSPD1 3329
Co-fractionation Homo sapiens
150 SNRPD3 6634
Co-fractionation Homo sapiens
151 HSPA9 3313
Co-fractionation Homo sapiens
152 LMNA 4000
Affinity Capture-MS Homo sapiens
153 LIG3 3980
Cross-Linking-MS (XL-MS) Homo sapiens
154 FTSJ3 117246
Proximity Label-MS Homo sapiens
155 SF3A3 10946
Affinity Capture-MS Homo sapiens
156 RBM17 84991
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
157 HUWE1 10075
Affinity Capture-MS Homo sapiens
158 PIN4 5303
Co-fractionation Homo sapiens
159 PIGS 94005
Affinity Capture-MS Homo sapiens
160 U2SURP 23350
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
161 PA2G4 5036
Affinity Capture-MS Homo sapiens
162 LOC100128588  
Co-fractionation Homo sapiens
163 DHX15 1665
Co-fractionation Homo sapiens
164 PARP1 142
Proximity Label-MS Homo sapiens
165 RPP30 10556
Proximity Label-MS Homo sapiens
166 SRRM1 10250
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
167 Psmb4 19172
Affinity Capture-MS Mus musculus
168 RPL6 6128
Affinity Capture-MS Homo sapiens
169 RPS21 6227
Affinity Capture-MS Homo sapiens
170 ERH 2079
Proximity Label-MS Homo sapiens
171 Dync1li1 235661
Affinity Capture-MS Mus musculus
172 SSRP1 6749
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
173 EIF1AX 1964
Affinity Capture-MS Homo sapiens
174 MRPS31  
Affinity Capture-MS Homo sapiens
175 IKBKG 8517
Reconstituted Complex Homo sapiens
176 RPS12 6206
Affinity Capture-MS Homo sapiens
177 DCTN2 10540
Affinity Capture-MS Homo sapiens
178 FKBP4 2288
Cross-Linking-MS (XL-MS) Homo sapiens
179 Sf3a1 67465
Affinity Capture-MS Mus musculus
180 BAZ2A  
Proximity Label-MS Homo sapiens
181 TBL3 10607
Proximity Label-MS Homo sapiens
182 PUF60 22827
Co-fractionation Homo sapiens
183 CCDC97  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
184 NUDT8  
Affinity Capture-MS Homo sapiens
185 SIRT1  
Proximity Label-MS Homo sapiens
186 NIFK 84365
Proximity Label-MS Homo sapiens
187 RPL34 6164
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
188 MMGT1 93380
Affinity Capture-MS Homo sapiens
189 TRAP1 10131
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
190 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
191 PDLIM5 10611
Co-fractionation Homo sapiens
192 NCL 4691
Affinity Capture-MS Homo sapiens
193 SF3B4 10262
Affinity Capture-MS Homo sapiens
194 RPL7A 6130
Affinity Capture-MS Homo sapiens
195 CENPC  
Proximity Label-MS Homo sapiens
196 ANXA5 308
Affinity Capture-MS Homo sapiens
197 RPS6 6194
Proximity Label-MS Homo sapiens
198 ANKRD11 29123
Proximity Label-MS Homo sapiens
199 C1QBP 708
Affinity Capture-MS Homo sapiens
200 HSPA4L 22824
Co-fractionation Homo sapiens
201 ANXA2 302
Co-fractionation Homo sapiens
202 SNRPA1 6627
Co-fractionation Homo sapiens
203 RPA3 6119
Proximity Label-MS Homo sapiens
204 NEDD8 4738
Co-fractionation Homo sapiens
205 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
206 Chmp3  
Affinity Capture-MS Mus musculus
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here