Gene ontology annotations for RPS21
Experiment description of studies that identified RPS21 in sEVs
1
Experiment ID
489
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
490
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
491
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
492
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
412
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MCF7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
6
Experiment ID
414
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
7
Experiment ID
426
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
8
Experiment ID
427
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
9
Experiment ID
497
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
10
Experiment ID
498
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
11
Experiment ID
20
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
17956143
Organism
Homo sapiens
Experiment description
Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors
"Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name
JPR
Publication year
2007
Sample
Colorectal cancer cells
Sample name
HT29
Isolation/purification methods
Differential centrifugation Sucrose density gradient Diafiltration
Flotation density
1.16 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
12
Experiment ID
207
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
13
Experiment ID
208
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
14
Experiment ID
209
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
15
Experiment ID
407
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
16
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
17
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
18
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
19
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
20
Experiment ID
405
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Foreskin fibroblasts
Sample name
BJ
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
21
Experiment ID
363
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
22
Experiment ID
364
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
23
Experiment ID
365
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
24
Experiment ID
417
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Lymphoma cells
Sample name
Raji
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
25
Experiment ID
411
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Mammary cancer-associated fibroblasts
Sample name
mCAF
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
26
Experiment ID
418
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
27
Experiment ID
418
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
28
Experiment ID
413
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Normal mammary epithelial cells
Sample name
MCF10A
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
29
Experiment ID
406
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
BxPC3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
30
Experiment ID
415
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
31
Experiment ID
434
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
32
Experiment ID
435
MISEV standards
✘
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
33
Experiment ID
408
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPDE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
34
Experiment ID
409
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPNE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
35
Experiment ID
416
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pluripotent stem cells
Sample name
PSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
36
Experiment ID
410
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✔
Negative markers
✔
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
T lymphocytes
Sample name
Jurkat
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
37
Experiment ID
217
MISEV standards
✔
Biophysical techniques
✔
Enriched markers
✘
Negative markers
✔
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for RPS21
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
RPSA
3921
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Two-hybrid
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
2
SLFN11
91607
Affinity Capture-MS
Homo sapiens
3
RIT1
6016
Negative Genetic
Homo sapiens
4
RPSAP58
388524
Affinity Capture-MS
Homo sapiens
5
UBE2H
7328
Affinity Capture-MS
Homo sapiens
6
SLIT2
9353
Affinity Capture-MS
Homo sapiens
7
RIOK1
83732
Co-crystal Structure
Homo sapiens
8
ATPIF1
93974
Affinity Capture-MS
Homo sapiens
9
UBL4A
8266
Affinity Capture-MS
Homo sapiens
10
RPS28
6234
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
11
DNAJC8
22826
Affinity Capture-MS
Homo sapiens
12
RPS3A
6189
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
13
TCEB3
6924
Affinity Capture-MS
Homo sapiens
14
RPL30
6156
Co-fractionation
Homo sapiens
15
RPS17
6218
Co-fractionation
Homo sapiens
16
PSMD11
5717
Co-fractionation
Homo sapiens
17
RPS2
6187
Co-fractionation
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
18
RPL37A
6168
Co-fractionation
Homo sapiens
19
G3BP1
10146
Affinity Capture-MS
Homo sapiens
20
THOC2
57187
Affinity Capture-MS
Homo sapiens
21
RPS19
6223
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
22
RPL10
6134
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
23
RPL15
6138
Co-fractionation
Homo sapiens
24
RPL14
9045
Co-fractionation
Homo sapiens
25
KARS
3735
Affinity Capture-MS
Homo sapiens
26
RIOK3
8780
Affinity Capture-MS
Homo sapiens
27
CAND1
55832
Affinity Capture-MS
Homo sapiens
28
SOX2
Affinity Capture-MS
Homo sapiens
29
APP
351
Reconstituted Complex
Homo sapiens
30
RPS3
6188
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
31
ATG13
9776
Affinity Capture-MS
Homo sapiens
32
NUDCD2
134492
Co-fractionation
Homo sapiens
33
EIF4ENIF1
56478
Two-hybrid
Homo sapiens
34
TOP2A
7153
Affinity Capture-MS
Homo sapiens
35
RPS20
6224
Co-fractionation
Homo sapiens
36
TMA16
Affinity Capture-MS
Homo sapiens
37
EIF3B
8662
Affinity Capture-MS
Homo sapiens
38
COPS5
10987
Affinity Capture-MS
Homo sapiens
39
METAP2
10988
Affinity Capture-MS
Homo sapiens
40
RPL24
6152
Co-fractionation
Homo sapiens
41
NPM1
4869
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
42
RPS8
6202
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
43
PSMC6
5706
Co-fractionation
Homo sapiens
44
SRP19
6728
Affinity Capture-MS
Homo sapiens
45
EIF5B
9669
Cross-Linking-MS (XL-MS)
Homo sapiens
46
RPS5
6193
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
47
RPS9
6203
Co-fractionation
Homo sapiens
48
GSPT1
2935
Affinity Capture-MS
Homo sapiens
49
Ufl1
Affinity Capture-MS
Mus musculus
50
FAM207A
Co-crystal Structure
Homo sapiens
51
DYNLL1
8655
Co-fractionation
Homo sapiens
52
ITGA4
3676
Affinity Capture-MS
Homo sapiens
53
SRP68
6730
Affinity Capture-MS
Homo sapiens
54
VCAM1
7412
Affinity Capture-MS
Homo sapiens
55
DDX6
1656
Affinity Capture-MS
Homo sapiens
56
SFN
2810
Affinity Capture-MS
Homo sapiens
57
PSMC1
5700
Co-fractionation
Homo sapiens
58
Eif3a
13669
Affinity Capture-MS
Mus musculus
59
CUL1
8454
Affinity Capture-MS
Homo sapiens
60
ARIH2
10425
Affinity Capture-MS
Homo sapiens
61
TACO1
Affinity Capture-MS
Homo sapiens
62
RPS11
6205
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
63
NACA2
342538
Co-fractionation
Homo sapiens
64
TP53BP1
7158
Affinity Capture-MS
Homo sapiens
65
RPL10L
140801
Co-fractionation
Homo sapiens
66
MED4
29079
Cross-Linking-MS (XL-MS)
Homo sapiens
67
EIF2S3
1968
Affinity Capture-MS
Homo sapiens
68
CHMP4C
92421
Affinity Capture-MS
Homo sapiens
69
RPS23
6228
Co-fractionation
Homo sapiens
70
RPL27
6155
Co-fractionation
Homo sapiens
71
SRP9
6726
Affinity Capture-MS
Homo sapiens
72
RAD23B
5887
Co-fractionation
Homo sapiens
73
PSMC2
5701
Co-fractionation
Homo sapiens
74
PIP4K2C
79837
Affinity Capture-MS
Homo sapiens
75
FHL5
9457
Two-hybrid
Homo sapiens
76
RIN3
Affinity Capture-MS
Homo sapiens
77
RPL9
6133
Co-fractionation
Homo sapiens
78
NME2P1
283458
Affinity Capture-MS
Homo sapiens
79
RPL4
6124
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
80
DRG1
4733
Affinity Capture-MS
Homo sapiens
81
CLNS1A
1207
Co-fractionation
Homo sapiens
82
DNAJC2
27000
Affinity Capture-MS
Homo sapiens
83
YTHDF3
Two-hybrid
Homo sapiens
84
ADRM1
11047
Co-fractionation
Homo sapiens
85
HIST1H3E
8353
Affinity Capture-MS
Homo sapiens
86
RPL5
6125
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
87
RPL18A
6142
Co-fractionation
Homo sapiens
88
PABPC1
26986
Co-fractionation
Homo sapiens
89
PSMD2
5708
Co-fractionation
Homo sapiens
90
RPS10
6204
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
91
RPL31
6160
Co-fractionation
Homo sapiens
92
RPS7
6201
Co-fractionation
Homo sapiens
93
RPS24
6229
Co-fractionation
Homo sapiens
94
SYNCRIP
10492
Co-fractionation
Homo sapiens
95
RPLP1
6176
Co-fractionation
Homo sapiens
96
CSNK1E
1454
Affinity Capture-MS
Homo sapiens
97
RPS6
6194
Co-fractionation
Homo sapiens
98
PLEKHF2
79666
Two-hybrid
Homo sapiens
99
ENY2
56943
Affinity Capture-MS
Homo sapiens
100
RPLP0
6175
Co-fractionation
Homo sapiens
101
LYAR
55646
Affinity Capture-MS
Homo sapiens
102
RPLP0P6
220717
Co-fractionation
Homo sapiens
103
TRIM31
Affinity Capture-MS
Homo sapiens
104
RPS15A
6210
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
105
RPS29
6235
Co-fractionation
Homo sapiens
106
SEC61B
10952
Affinity Capture-MS
Homo sapiens
107
CYLD
Affinity Capture-MS
Homo sapiens
108
HMGB2
3148
Affinity Capture-MS
Homo sapiens
109
SLC25A5
292
Co-fractionation
Homo sapiens
110
RPL11
6135
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
111
UCHL5
51377
Co-fractionation
Homo sapiens
112
ANLN
54443
Affinity Capture-MS
Homo sapiens
113
RPLP2
6181
Co-fractionation
Homo sapiens
114
FOXA1
Affinity Capture-MS
Homo sapiens
115
RPL23
9349
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
116
ATP5I
521
Co-fractionation
Homo sapiens
117
BYSL
705
Affinity Capture-MS
Homo sapiens
Co-crystal Structure
Homo sapiens
118
RC3H2
Affinity Capture-MS
Homo sapiens
119
ECT2
1894
Affinity Capture-MS
Homo sapiens
120
PSMD13
5719
Co-fractionation
Homo sapiens
121
PSMD8
5714
Co-fractionation
Homo sapiens
122
CTTN
2017
Affinity Capture-MS
Homo sapiens
123
CTCF
Affinity Capture-MS
Homo sapiens
124
CUL3
8452
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
125
CIT
11113
Affinity Capture-MS
Homo sapiens
126
RPL21
6144
Co-fractionation
Homo sapiens
127
CSNK2A1
1457
Affinity Capture-MS
Homo sapiens
128
HNRNPA1
3178
Co-fractionation
Homo sapiens
129
MYC
Affinity Capture-MS
Homo sapiens
130
RPL7
6129
Co-fractionation
Homo sapiens
131
FN1
2335
Affinity Capture-MS
Homo sapiens
132
RPS27
6232
Co-fractionation
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
133
CDK2
1017
Affinity Capture-MS
Homo sapiens
134
PIP4K2A
5305
Affinity Capture-MS
Homo sapiens
135
NCAPH
23397
Affinity Capture-MS
Homo sapiens
136
RBM42
Affinity Capture-MS
Homo sapiens
137
PSPC1
55269
Affinity Capture-MS
Homo sapiens
138
RPA3
6119
Proximity Label-MS
Homo sapiens
139
FXR2
9513
Affinity Capture-MS
Homo sapiens
140
RPS13
6207
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
141
PHF6
Affinity Capture-MS
Homo sapiens
142
RPL12
6136
Co-fractionation
Homo sapiens
143
RPL22
6146
Co-fractionation
Homo sapiens
144
MYCN
Affinity Capture-MS
Homo sapiens
145
RPL18
6141
Co-fractionation
Homo sapiens
146
DDRGK1
65992
Affinity Capture-MS
Homo sapiens
147
RPS18
6222
Co-fractionation
Homo sapiens
148
Rpl35
66489
Affinity Capture-MS
Mus musculus
149
CSDE1
7812
Cross-Linking-MS (XL-MS)
Homo sapiens
150
PSMD1
5707
Co-fractionation
Homo sapiens
151
Eif3e
16341
Affinity Capture-MS
Mus musculus
152
TSR1
55720
Affinity Capture-MS
Homo sapiens
153
CA2
760
Affinity Capture-MS
Homo sapiens
154
C9orf72
Affinity Capture-MS
Homo sapiens
155
RPS16
6217
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
156
RPL19
6143
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
157
RPL13
6137
Co-fractionation
Homo sapiens
158
SRP54
6729
Cross-Linking-MS (XL-MS)
Homo sapiens
159
RAD21
5885
Affinity Capture-MS
Homo sapiens
160
CCDC68
Co-fractionation
Homo sapiens
161
RPS25
6230
Co-fractionation
Homo sapiens
162
ILF3
3609
Affinity Capture-MS
Homo sapiens
163
Osgep
Affinity Capture-MS
Mus musculus
164
RPL6
6128
Co-fractionation
Homo sapiens
165
C9orf114
Affinity Capture-MS
Homo sapiens
166
CLCA2
Co-fractionation
Homo sapiens
167
UBA52
7311
Co-fractionation
Homo sapiens
168
RPS12
6206
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
169
HNRNPM
4670
Co-fractionation
Homo sapiens
170
RPS4X
6191
Reconstituted Complex
Homo sapiens
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
171
ABL1
25
Affinity Capture-MS
Homo sapiens
172
SRP72
6731
Affinity Capture-MS
Homo sapiens
173
CAPRIN1
4076
Affinity Capture-MS
Homo sapiens
174
CAPZB
832
Affinity Capture-MS
Homo sapiens
175
G3BP2
9908
Affinity Capture-MS
Homo sapiens
176
MTHFD1
4522
Co-fractionation
Homo sapiens
177
TMLHE
55217
Affinity Capture-MS
Homo sapiens
178
DDX58
23586
Affinity Capture-RNA
Homo sapiens
179
MRPS21
Co-fractionation
Homo sapiens
180
RPL34
6164
Co-fractionation
Homo sapiens
181
RPS26
6231
Co-fractionation
Homo sapiens
182
UFL1
23376
Affinity Capture-MS
Homo sapiens
183
RPL10A
4736
Co-fractionation
Homo sapiens
184
ANXA11
311
Co-fractionation
Homo sapiens
185
DDX3X
1654
Co-fractionation
Homo sapiens
186
LRPAP1
4043
Affinity Capture-MS
Homo sapiens
187
TRIP4
9325
Affinity Capture-MS
Homo sapiens
188
RPL7A
6130
Co-fractionation
Homo sapiens
189
RBM8A
9939
Affinity Capture-MS
Homo sapiens
190
Rrbp1
Affinity Capture-MS
Mus musculus
191
RPS27L
51065
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
192
ZUFSP
221302
Two-hybrid
Homo sapiens
193
RPL23A
6147
Co-fractionation
Homo sapiens
194
KRAS
3845
Negative Genetic
Homo sapiens
195
HNRNPU
3192
Affinity Capture-MS
Homo sapiens
196
GNB2L1
10399
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
197
AR
367
Proximity Label-MS
Homo sapiens
198
RC3H1
149041
Affinity Capture-MS
Homo sapiens
199
MAST3
Affinity Capture-MS
Homo sapiens
200
LRCH3
84859
Cross-Linking-MS (XL-MS)
Homo sapiens
201
UBE2V2
7336
Co-fractionation
Homo sapiens
202
RPL17
6139
Co-fractionation
Homo sapiens
203
EIF5
1983
Affinity Capture-MS
Homo sapiens
204
PSMD3
5709
Co-fractionation
Homo sapiens
View the network
image/svg+xml
Pathways in which RPS21 is involved