Gene description for PIP4K2C
Gene name phosphatidylinositol-5-phosphate 4-kinase, type II, gamma
Gene symbol PIP4K2C
Other names/aliases PIP5K2C
Species Homo sapiens
 Database cross references - PIP4K2C
ExoCarta ExoCarta_79837
Vesiclepedia VP_79837
Entrez Gene 79837
HGNC 23786
UniProt Q8TBX8  
 PIP4K2C identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for PIP4K2C
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    1-phosphatidylinositol-4-phosphate 5-kinase activity GO:0016308 IDA
    1-phosphatidylinositol-5-phosphate 4-kinase activity GO:0016309 IBA
    identical protein binding GO:0042802 IPI
Biological Process
    regulation of autophagy GO:0010506 IMP
    negative regulation of insulin receptor signaling pathway GO:0046627 IMP
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IBA
    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process GO:1902635 IDA
    positive regulation of autophagosome assembly GO:2000786 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    autophagosome GO:0005776 IMP
    endoplasmic reticulum GO:0005783 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PIP4K2C in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
10
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PIP4K2C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 Mdm2  
Affinity Capture-MS Mus musculus
3 CAMKV 79012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 ACTR2 10097
Affinity Capture-MS Homo sapiens
5 NOS1AP  
Affinity Capture-MS Homo sapiens
6 PRPF38A 84950
Affinity Capture-MS Homo sapiens
7 FBXO25  
Biochemical Activity Homo sapiens
8 EPB41L5 57669
Affinity Capture-MS Homo sapiens
9 ARL6IP4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 APBA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 USP9X 8239
Affinity Capture-MS Homo sapiens
12 BRD2  
Affinity Capture-MS Homo sapiens
13 KARS 3735
Affinity Capture-MS Homo sapiens
14 ATG4C  
Affinity Capture-MS Homo sapiens
15 CAB39 51719
Affinity Capture-MS Homo sapiens
16 TCEB3 6924
Affinity Capture-MS Homo sapiens
17 DDX23 9416
Co-fractionation Homo sapiens
18 GSPT2 23708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RNPS1 10921
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 RASA2 5922
Affinity Capture-MS Homo sapiens
21 AFF4 27125
Affinity Capture-MS Homo sapiens
22 NOLC1 9221
Affinity Capture-MS Homo sapiens
23 LIG1 3978
Co-fractionation Homo sapiens
24 SCRIB 23513
Affinity Capture-MS Homo sapiens
25 PIP4K2B 8396
Affinity Capture-MS Homo sapiens
26 POC1B 282809
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TNFRSF19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 SYT2  
Affinity Capture-MS Homo sapiens
29 SUN2 25777
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TULP3 7289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 BRD4 23476
Affinity Capture-MS Homo sapiens
32 ASH2L 9070
Affinity Capture-MS Homo sapiens
33 SNX27 81609
Affinity Capture-MS Homo sapiens
34 ARHGAP22 58504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CSNK2B 1460
Co-fractionation Homo sapiens
36 CHERP 10523
Affinity Capture-MS Homo sapiens
37 YWHAB 7529
Co-fractionation Homo sapiens
38 MYL9 10398
Co-fractionation Homo sapiens
39 EPB41L3 23136
Affinity Capture-MS Homo sapiens
40 NF2 4771
Affinity Capture-MS Homo sapiens
41 PIP4K2C 79837
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
42 PRPF8 10594
Co-fractionation Homo sapiens
43 CUL4B 8450
Affinity Capture-MS Homo sapiens
44 PARK2  
Affinity Capture-MS Homo sapiens
45 STRADA 92335
Affinity Capture-MS Homo sapiens
46 RTF1 23168
Affinity Capture-MS Homo sapiens
47 HIF1AN 55662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 NCAPD3 23310
Co-fractionation Homo sapiens
49 OGT 8473
Reconstituted Complex Homo sapiens
50 RIOK2 55781
Affinity Capture-MS Homo sapiens
51 UBTF 7343
Affinity Capture-MS Homo sapiens
52 GPBP1L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 AP3B1 8546
Affinity Capture-MS Homo sapiens
54 CT45A5  
Affinity Capture-MS Homo sapiens
55 KIF1B 23095
Co-fractionation Homo sapiens
56 TNFAIP8L1  
Affinity Capture-MS Homo sapiens
57 AHCYL1 10768
Affinity Capture-MS Homo sapiens
58 PINK1  
Affinity Capture-MS Homo sapiens
59 CD6 923
Affinity Capture-MS Homo sapiens
60 TNFRSF1A 7132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 MADD 8567
Affinity Capture-MS Homo sapiens
62 CSNK2A1 1457
Co-fractionation Homo sapiens
63 HSD17B10 3028
Affinity Capture-MS Homo sapiens
64 DCAF16 54876
Affinity Capture-MS Homo sapiens
65 CCDC85A  
Affinity Capture-MS Homo sapiens
66 LZTS2 84445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
69 RPA3 6119
Proximity Label-MS Homo sapiens
70 WWC1  
Affinity Capture-MS Homo sapiens
71 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
72 C12orf43  
Affinity Capture-MS Homo sapiens
73 RFWD3  
Affinity Capture-MS Homo sapiens
74 HSPD1 3329
Affinity Capture-MS Homo sapiens
75 HAT1 8520
Co-fractionation Homo sapiens
76 BRD3 8019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 DIP2C 22982
Affinity Capture-MS Homo sapiens
78 APP 351
Reconstituted Complex Homo sapiens
79 PRICKLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 JUP 3728
Co-fractionation Homo sapiens
81 KIF2A 3796
Co-fractionation Homo sapiens
82 ZNF428  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 MTPN 136319
Co-fractionation Homo sapiens
84 ZRANB2 9406
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 MAX  
Affinity Capture-MS Homo sapiens
86 RPS21 6227
Affinity Capture-MS Homo sapiens
87 C9orf114  
Affinity Capture-MS Homo sapiens
88 PLCD3 113026
Affinity Capture-MS Homo sapiens
89 EDEM1  
Affinity Capture-MS Homo sapiens
90 SKP1 6500
Co-fractionation Homo sapiens
91 KNOP1 400506
Affinity Capture-MS Homo sapiens
92 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
93 RBMX 27316
Co-fractionation Homo sapiens
94 WIF1 11197
Affinity Capture-MS Homo sapiens
95 AHCYL2 23382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
97 LRPAP1 4043
Affinity Capture-MS Homo sapiens
98 EAF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 ZCCHC10  
Affinity Capture-MS Homo sapiens
100 GOT1 2805
Affinity Capture-MS Homo sapiens
101 STK11 6794
Affinity Capture-MS Homo sapiens
102 C16orf71  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 PTCH1  
Affinity Capture-MS Homo sapiens
104 PAGE1  
Affinity Capture-MS Homo sapiens
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