Gene description for AP3B1
Gene name adaptor-related protein complex 3, beta 1 subunit
Gene symbol AP3B1
Other names/aliases ADTB3
ADTB3A
HPS
HPS2
PE
Species Homo sapiens
 Database cross references - AP3B1
ExoCarta ExoCarta_8546
Vesiclepedia VP_8546
Entrez Gene 8546
HGNC 566
MIM 603401
UniProt O00203  
 AP3B1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Thymus 23844026    
 Gene ontology annotations for AP3B1
Molecular Function
    protein binding GO:0005515 IPI
    protein phosphatase binding GO:0019903 IPI
    GTP-dependent protein binding GO:0030742 IPI
Biological Process
    cell morphogenesis GO:0000902 IEA
    toll-like receptor signaling pathway GO:0002224 IEA
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    respiratory system process GO:0003016 IEA
    protein targeting to lysosome GO:0006622 IEA
    intracellular zinc ion homeostasis GO:0006882 IEA
    intracellular protein transport GO:0006886 TAS
    inflammatory response GO:0006954 IEA
    lysosome organization GO:0007040 IEA
    spermatogenesis GO:0007283 IEA
    single fertilization GO:0007338 IEA
    blood coagulation GO:0007596 IEA
    anterograde axonal transport GO:0008089 ISS
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 NAS
    granulocyte differentiation GO:0030851 IEA
    melanosome organization GO:0032438 IMP
    protein localization to cell surface GO:0034394 IEA
    clathrin-coated vesicle cargo loading, AP-3-mediated GO:0035654 NAS
    protein modification process GO:0036211 IEA
    mRNA transcription by RNA polymerase II GO:0042789 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    intracellular transport GO:0046907 NAS
    antigen processing and presentation, exogenous lipid antigen via MHC class Ib GO:0048007 IEA
    anterograde synaptic vesicle transport GO:0048490 IBA
    anterograde synaptic vesicle transport GO:0048490 ISS
    homeostasis of number of cells GO:0048872 IEA
    positive regulation of NK T cell differentiation GO:0051138 IEA
    platelet dense granule organization GO:0060155 NAS
    lung morphogenesis GO:0060425 IEA
    establishment of protein localization to mitochondrial membrane involved in mitochondrial fission GO:0090152 IEA
    skin epidermis development GO:0098773 IEA
    melanosome assembly GO:1903232 NAS
Subcellular Localization
    mitochondrion GO:0005739 IEA
    lysosomal membrane GO:0005765 HDA
    early endosome GO:0005769 NAS
    Golgi apparatus GO:0005794 IEA
    membrane GO:0016020 HDA
    AP-3 adaptor complex GO:0030123 NAS
    clathrin adaptor complex GO:0030131 IEA
    clathrin-coated vesicle membrane GO:0030665 IEA
    axon cytoplasm GO:1904115 IEA
    microvesicle GO:1990742 IEA
 Experiment description of studies that identified AP3B1 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 207
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 208
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 1203
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
13
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
21
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AP3B1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ATR  
Protein-peptide Homo sapiens
3 CAMKV 79012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MCM3 4172
Affinity Capture-MS Homo sapiens
5 HDAC4  
Affinity Capture-MS Homo sapiens
6 ARHGEF2 9181
Co-fractionation Homo sapiens
7 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SLFN11 91607
Proximity Label-MS Homo sapiens
9 ATG9A 79065
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
10 PRPF38A 84950
Affinity Capture-MS Homo sapiens
11 FKBP15 23307
Co-fractionation Homo sapiens
12 EPB41L5 57669
Affinity Capture-MS Homo sapiens
13 ARF3 377
Proximity Label-MS Homo sapiens
14 C9orf78 51759
Affinity Capture-MS Homo sapiens
15 FAM21A 387680
Co-fractionation Homo sapiens
16 ATM 472
Protein-peptide Homo sapiens
17 PNMAL1  
Affinity Capture-MS Homo sapiens
18 SYVN1 84447
Affinity Capture-MS Homo sapiens
19 CLTB 1212
Proximity Label-MS Homo sapiens
20 CAPZB 832
Co-fractionation Homo sapiens
21 Lyplal1  
Affinity Capture-MS Mus musculus
22 YWHAZ 7534
Affinity Capture-MS Homo sapiens
23 DYRK1A 1859
Affinity Capture-MS Homo sapiens
24 AP3S2 10239
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
25 NOLC1 9221
Affinity Capture-MS Homo sapiens
26 NFKBIL1  
Affinity Capture-MS Homo sapiens
27 DUSP14 11072
Affinity Capture-MS Homo sapiens
28 AGO3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 ZNF460  
Affinity Capture-MS Homo sapiens
30 SYT2  
Affinity Capture-MS Homo sapiens
31 LRSAM1 90678
Affinity Capture-Western Homo sapiens
32 ATRX 546
Affinity Capture-MS Homo sapiens
33 CLTC 1213
Co-fractionation Homo sapiens
34 ERMAP 114625
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 NEDD4 4734
Co-fractionation Homo sapiens
36 FAM20C 56975
Affinity Capture-MS Homo sapiens
37 RBM14 10432
Affinity Capture-MS Homo sapiens
38 KIF14 9928
Affinity Capture-MS Homo sapiens
39 KIAA0196 9897
Co-fractionation Homo sapiens
40 PES1 23481
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 EPB41L3 23136
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 LDHB 3945
Cross-Linking-MS (XL-MS) Homo sapiens
43 AP3M1 26985
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
44 JMJD6 23210
Co-fractionation Homo sapiens
45 POLD1 5424
Affinity Capture-MS Homo sapiens
46 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
47 CAPZA1 829
Co-fractionation Homo sapiens
48 PARK2  
Affinity Capture-MS Homo sapiens
49 PLD1 5337
Affinity Capture-MS Homo sapiens
50 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ARHGEF1 9138
Co-fractionation Homo sapiens
52 DNAJC5 80331
Proximity Label-MS Homo sapiens
53 TBC1D32 221322
Proximity Label-MS Homo sapiens
54 DDX20 11218
Affinity Capture-MS Homo sapiens
55 KDM4B  
Affinity Capture-MS Homo sapiens
56 FAM9A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 NTRK1 4914
Affinity Capture-MS Homo sapiens
58 FGF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 SNX11 29916
Affinity Capture-MS Homo sapiens
60 CREB3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 COG3 83548
Co-fractionation Homo sapiens
62 SLC30A3  
Reconstituted Complex Homo sapiens
63 TMEM63A 9725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 FGFRL1 53834
Affinity Capture-MS Homo sapiens
65 PELP1 27043
Co-fractionation Homo sapiens
66 EIF2AK1  
Affinity Capture-MS Homo sapiens
67 KIAA1033 23325
Co-fractionation Homo sapiens
68 LRRTM4  
Affinity Capture-MS Homo sapiens
69 CT45A5  
Affinity Capture-MS Homo sapiens
70 FGFBP1 9982
Affinity Capture-MS Homo sapiens
71 EPB41L1 2036
Affinity Capture-MS Homo sapiens
72 CDC73  
Co-fractionation Homo sapiens
73 XPO1 7514
Affinity Capture-MS Homo sapiens
74 DOCK7 85440
Co-fractionation Homo sapiens
75 ALPI  
Biochemical Activity Bos taurus
76 NR3C1 2908
Proximity Label-MS Homo sapiens
77 ARF6 382
Reconstituted Complex Homo sapiens
78 NAF1  
Affinity Capture-MS Homo sapiens
79 CREB3L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 MFAP1  
Affinity Capture-MS Homo sapiens
82 AP3M2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
83 NLRP3  
Affinity Capture-MS Homo sapiens
84 CREB3L3  
Affinity Capture-MS Homo sapiens
85 TFPI2 7980
Affinity Capture-MS Homo sapiens
86 YIPF4 84272
Co-fractionation Homo sapiens
87 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
88 SKA3  
Affinity Capture-MS Homo sapiens
89 CHD8 57680
Co-fractionation Homo sapiens
90 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 SEPT7 989
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
92 NHLH2  
Affinity Capture-MS Homo sapiens
93 RBBP8  
Affinity Capture-MS Homo sapiens
94 AP3S1 1176
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
95 GRK5 2869
Affinity Capture-MS Homo sapiens
96 MRPL42  
Affinity Capture-MS Homo sapiens
97 ARRB2 409
Affinity Capture-MS Homo sapiens
98 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
99 CCDC154  
Affinity Capture-MS Homo sapiens
100 LAMTOR1 55004
Proximity Label-MS Homo sapiens
101 TP53 7157
Affinity Capture-MS Homo sapiens
102 DLG3 1741
Affinity Capture-MS Homo sapiens
103 HSPA8 3312
Affinity Capture-MS Homo sapiens
104 ARF5 381
Reconstituted Complex Homo sapiens
105 PLCD3 113026
Affinity Capture-MS Homo sapiens
106 UTP23  
Affinity Capture-MS Homo sapiens
107 PLEKHJ1  
Affinity Capture-MS Homo sapiens
108 EPB41L2 2037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 PTGER3  
Affinity Capture-MS Homo sapiens
110 WIF1 11197
Affinity Capture-MS Homo sapiens
111 EDA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 SEC62 7095
Affinity Capture-MS Homo sapiens
113 ARRB1 408
Affinity Capture-MS Homo sapiens
114 ZNRF4  
Affinity Capture-MS Homo sapiens
115 EAF1  
Affinity Capture-MS Homo sapiens
116 CLTA 1211
Proximity Label-MS Homo sapiens
117 AP3B2 8120
Cross-Linking-MS (XL-MS) Homo sapiens
118 SNW1 22938
Affinity Capture-MS Homo sapiens
119 Nup155  
Affinity Capture-MS Mus musculus
120 PAF1 54623
Co-fractionation Homo sapiens
121 VASN 114990
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 AP3D1 8943
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
123 RPA3 6119
Proximity Label-MS Homo sapiens
124 CDK11A 728642
Co-fractionation Homo sapiens
125 CUL4A 8451
Affinity Capture-MS Homo sapiens
126 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here