Gene description for LRSAM1
Gene name leucine rich repeat and sterile alpha motif containing 1
Gene symbol LRSAM1
Other names/aliases CMT2P
RIFLE
TAL
Species Homo sapiens
 Database cross references - LRSAM1
ExoCarta ExoCarta_90678
Vesiclepedia VP_90678
Entrez Gene 90678
HGNC 25135
MIM 610933
UniProt Q6UWE0  
 LRSAM1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 19837982    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for LRSAM1
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IDA
Biological Process
    protein polyubiquitination GO:0000209 IDA
    autophagy GO:0006914 IEA
    protein catabolic process GO:0030163 IMP
    negative regulation of endocytosis GO:0045806 IMP
    viral budding GO:0046755 IMP
    protein autoubiquitination GO:0051865 IDA
    ubiquitin-dependent endocytosis GO:0070086 IDA
    positive regulation of xenophagy GO:1904417 IMP
    positive regulation of autophagosome assembly GO:2000786 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified LRSAM1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LRSAM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Co-fractionation Homo sapiens
2 UBE2H 7328
Two-hybrid Homo sapiens
3 FAM178B 51252
Two-hybrid Homo sapiens
4 ATXN1 6310
Two-hybrid Homo sapiens
5 UBE2W  
Two-hybrid Homo sapiens
6 KCNH7  
Affinity Capture-MS Homo sapiens
7 USP2  
Two-hybrid Homo sapiens
8 ISCA1  
Affinity Capture-MS Homo sapiens
9 UBE2N 7334
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 HSD17B4 3295
Affinity Capture-MS Homo sapiens
11 XAF1  
Two-hybrid Homo sapiens
12 UEVLD 55293
Two-hybrid Homo sapiens
13 CUL3 8452
Affinity Capture-MS Homo sapiens
14 EGFR 1956
Affinity Capture-Western Homo sapiens
15 EHD4 30844
Co-fractionation Homo sapiens
16 UTP6  
Affinity Capture-MS Homo sapiens
17 CTPS2 56474
Co-fractionation Homo sapiens
18 NINL  
Two-hybrid Homo sapiens
19 GPR108 56927
Two-hybrid Homo sapiens
20 UBE3A 7337
Co-fractionation Homo sapiens
21 ZMAT4  
Two-hybrid Homo sapiens
22 HSPBP1 23640
Co-fractionation Homo sapiens
23 ATXN7  
Two-hybrid Homo sapiens
24 DR1  
Co-fractionation Homo sapiens
25 ACTA1 58
Affinity Capture-MS Homo sapiens
26 MAGEA1  
Two-hybrid Homo sapiens
27 TSG101 7251
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
28 DBNL 28988
Co-fractionation Homo sapiens
29 ERO1L 30001
Co-fractionation Homo sapiens
30 MKRN3  
Two-hybrid Homo sapiens
31 UBE2E1  
Two-hybrid Homo sapiens
32 USP21  
Two-hybrid Homo sapiens
33 UBE2D4  
Two-hybrid Homo sapiens
34 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
35 P3H1 64175
Co-fractionation Homo sapiens
36 PAPOLG  
Affinity Capture-MS Homo sapiens
37 MDM4  
Two-hybrid Homo sapiens
38 LIPF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 RAI14 26064
Affinity Capture-MS Homo sapiens
40 TRIM74  
Two-hybrid Homo sapiens
41 DMAP1 55929
Two-hybrid Homo sapiens
42 UBE2D2 7322
Two-hybrid Homo sapiens
43 UBE2I 7329
Two-hybrid Homo sapiens
44 TERF1 7013
Two-hybrid Homo sapiens
45 KLC1 3831
Two-hybrid Homo sapiens
46 DDX5 1655
Co-fractionation Homo sapiens
47 HSF1  
Co-fractionation Homo sapiens
48 UBE2K 3093
Two-hybrid Homo sapiens
49 RNF111 54778
Two-hybrid Homo sapiens
50 PLIN3 10226
Co-fractionation Homo sapiens
51 CNOT6L 246175
Affinity Capture-MS Homo sapiens
52 PPME1 51400
Co-fractionation Homo sapiens
53 SCRIB 23513
Affinity Capture-MS Homo sapiens
54 ANKMY2 57037
Co-fractionation Homo sapiens
55 UBE2D3 7323
Two-hybrid Homo sapiens
56 MTMR2 8898
Co-fractionation Homo sapiens
57 AP3B1 8546
Affinity Capture-Western Homo sapiens
58 MPRIP 23164
Affinity Capture-MS Homo sapiens
59 LRRC46  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 TRIM17  
Two-hybrid Homo sapiens
61 TMSB4Y  
Affinity Capture-MS Homo sapiens
62 CCDC6 8030
Two-hybrid Homo sapiens
63 LSS 4047
Affinity Capture-MS Homo sapiens
64 LRSAM1 90678
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
65 YTHDF1 54915
Co-fractionation Homo sapiens
66 GOPC 57120
Co-fractionation Homo sapiens
67 VPS11 55823
Two-hybrid Homo sapiens
68 UBE2J1 51465
Two-hybrid Homo sapiens
69 UBE2D1 7321
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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