Gene description for VPS11
Gene name vacuolar protein sorting 11 homolog (S. cerevisiae)
Gene symbol VPS11
Other names/aliases END1
PEP5
RNF108
hVPS11
Species Homo sapiens
 Database cross references - VPS11
ExoCarta ExoCarta_55823
Vesiclepedia VP_55823
Entrez Gene 55823
HGNC 14583
MIM 608549
UniProt Q9H270  
 VPS11 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for VPS11
Molecular Function
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IPI
    syntaxin binding GO:0019905 IDA
    protein-macromolecule adaptor activity GO:0030674 IBA
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IMP
Biological Process
    intracellular protein transport GO:0006886 IEA
    vesicle docking involved in exocytosis GO:0006904 IBA
    autophagy GO:0006914 IEA
    endosome organization GO:0007032 IBA
    vacuole organization GO:0007033 IBA
    endosome to lysosome transport GO:0008333 IMP
    protein ubiquitination GO:0016567 IMP
    regulation of protein stability GO:0031647 HMP
    negative regulation of intracellular estrogen receptor signaling pathway GO:0033147 IGI
    endosomal vesicle fusion GO:0034058 IMP
    endosomal vesicle fusion GO:0034058 NAS
    regulation of SNARE complex assembly GO:0035542 NAS
    positive regulation of protein catabolic process GO:0045732 IMP
    organelle fusion GO:0048284 IBA
    regulation of organelle assembly GO:1902115 IMP
    positive regulation of protein targeting to mitochondrion GO:1903955 HMP
    positive regulation of early endosome to late endosome transport GO:2000643 IMP
Subcellular Localization
    lysosome GO:0005764 IDA
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 HDA
    endosome GO:0005768 IBA
    endosome GO:0005768 IDA
    early endosome GO:0005769 IDA
    late endosome GO:0005770 IDA
    late endosome GO:0005770 IDA
    autophagosome GO:0005776 IEA
    endosome membrane GO:0010008 TAS
    clathrin-coated vesicle GO:0030136 IEA
    endocytic vesicle GO:0030139 IDA
    HOPS complex GO:0030897 IBA
    HOPS complex GO:0030897 IDA
    HOPS complex GO:0030897 NAS
    late endosome membrane GO:0031902 IEA
    CORVET complex GO:0033263 NAS
 Experiment description of studies that identified VPS11 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 834
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VPS39 23339
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 MARK3 4140
Two-hybrid Homo sapiens
3 KDM1A 23028
Two-hybrid Homo sapiens
4 RAB7A 7879
Reconstituted Complex Homo sapiens
5 GGA3 23163
Biochemical Activity Homo sapiens
6 VPS16 64601
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
7 VPS41 27072
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
8 C22orf29  
Affinity Capture-MS Homo sapiens
9 SCRIB 23513
Affinity Capture-MS Homo sapiens
10 EDNRA 1909
Co-purification Homo sapiens
11 LSM1 27257
Two-hybrid Homo sapiens
12 RILP 83547
Reconstituted Complex Homo sapiens
13 SCYL3 57147
Affinity Capture-MS Homo sapiens
14 RNF181  
Two-hybrid Homo sapiens
15 C15orf27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TGFBRAP1 9392
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 GTF2I 2969
Affinity Capture-MS Homo sapiens
18 Plk2  
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
19 ISOC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 UBE2L3 7332
Reconstituted Complex Homo sapiens
21 KRTAP10-3  
Two-hybrid Homo sapiens
22 EZR 7430
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 BCAR1 9564
Two-hybrid Homo sapiens
24 RLBP1  
Affinity Capture-MS Homo sapiens
25 TSG101 7251
Two-hybrid Homo sapiens
26 CCDC92  
Affinity Capture-MS Homo sapiens
27 CSTA 1475
Affinity Capture-MS Homo sapiens
28 PARL  
Affinity Capture-MS Homo sapiens
29 SHMT2 6472
Affinity Capture-RNA Homo sapiens
30 PSME1 5720
Affinity Capture-MS Homo sapiens
31 THRSP  
Affinity Capture-MS Homo sapiens
32 RNF7  
Affinity Capture-MS Homo sapiens
33 NXF1 10482
Affinity Capture-RNA Homo sapiens
34 VPS33A 65082
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
35 UVRAG  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 KRTAP10-8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
37 MON1B 22879
Two-hybrid Homo sapiens
38 RAB5A 5868
Proximity Label-MS Homo sapiens
39 WWP2 11060
Co-fractionation Homo sapiens
40 UBE2D2 7322
Reconstituted Complex Homo sapiens
41 S100A4 6275
Affinity Capture-MS Homo sapiens
42 CYS1 192668
Affinity Capture-MS Homo sapiens
43 RDX 5962
Reconstituted Complex Homo sapiens
44 MOV10 4343
Affinity Capture-RNA Homo sapiens
45 C3orf18  
Affinity Capture-MS Homo sapiens
46 CAMSAP3  
Affinity Capture-MS Homo sapiens
47 NTRK1 4914
Affinity Capture-MS Homo sapiens
48 RNF166  
Affinity Capture-MS Homo sapiens
49 RAB9A 9367
Proximity Label-MS Homo sapiens
50 ZNF263  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 TTC9C 283237
Affinity Capture-MS Homo sapiens
52 GRSF1 2926
Two-hybrid Homo sapiens
53 TRIM32 22954
Two-hybrid Homo sapiens
54 ZUFSP 221302
Affinity Capture-MS Homo sapiens
55 ARRDC3 57561
Affinity Capture-MS Homo sapiens
56 MID1IP1  
Affinity Capture-MS Homo sapiens
57 STX7 8417
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
58 PARVA 55742
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 BMI1  
Two-hybrid Homo sapiens
60 KIAA0368 23392
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
61 MSN 4478
Reconstituted Complex Homo sapiens
62 Vps41  
Affinity Capture-MS Mus musculus
63 C7orf60 154743
Affinity Capture-MS Homo sapiens
64 LRRC61 65999
Affinity Capture-MS Homo sapiens
65 VPS8 23355
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
66 STX17 55014
Affinity Capture-Western Homo sapiens
67 VPS18 57617
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 NOTCH1 4851
Affinity Capture-MS Homo sapiens
69 RANBP17  
Co-fractionation Homo sapiens
70 LRSAM1 90678
Two-hybrid Homo sapiens
71 UBE2U  
Two-hybrid Homo sapiens
72 TICAM1  
Affinity Capture-MS Homo sapiens
73 HYPM  
Affinity Capture-MS Homo sapiens
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