Gene description for CSTA
Gene name cystatin A (stefin A)
Gene symbol CSTA
Other names/aliases AREI
STF1
STFA
Species Homo sapiens
 Database cross references - CSTA
ExoCarta ExoCarta_1475
Vesiclepedia VP_1475
Entrez Gene 1475
HGNC 2481
MIM 184600
UniProt P01040  
 CSTA identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for CSTA
Molecular Function
    protease binding GO:0002020 IPI
    cysteine-type endopeptidase inhibitor activity GO:0004869 IBA
    cysteine-type endopeptidase inhibitor activity GO:0004869 IDA
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of peptidase activity GO:0010466 IDA
    peptide cross-linking GO:0018149 IDA
    keratinocyte differentiation GO:0030216 IDA
    negative regulation of proteolysis GO:0045861 IDA
    cell-cell adhesion GO:0098609 IMP
Subcellular Localization
    cornified envelope GO:0001533 IDA
    cornified envelope GO:0001533 TAS
    extracellular space GO:0005615 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    peptidase inhibitor complex GO:1904090 IPI
 Experiment description of studies that identified CSTA in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 266
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Wild type
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 267
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 268
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CSTA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MCM2 4171
Affinity Capture-MS Homo sapiens
2 TCEB1 6921
Affinity Capture-MS Homo sapiens
3 KRT1 3848
Reconstituted Complex Homo sapiens
4 PIWIL4  
Affinity Capture-MS Homo sapiens
5 FGD2 221472
Affinity Capture-MS Homo sapiens
6 TLK2  
Affinity Capture-MS Homo sapiens
7 TCTN3  
Affinity Capture-MS Homo sapiens
8 C15orf41  
Affinity Capture-MS Homo sapiens
9 CSTA 1475
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
10 ZIC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 KDM4A  
Affinity Capture-MS Homo sapiens
12 ST7  
Affinity Capture-MS Homo sapiens
13 PARP8  
Affinity Capture-MS Homo sapiens
14 KIAA1429 25962
Affinity Capture-MS Homo sapiens
15 WTAP 9589
Affinity Capture-MS Homo sapiens
16 SPECC1L 23384
Affinity Capture-MS Homo sapiens
17 HR  
Affinity Capture-MS Homo sapiens
18 NUDT3 11165
Affinity Capture-MS Homo sapiens
19 RAD51  
Affinity Capture-MS Homo sapiens
20 KPRP 448834
Affinity Capture-MS Homo sapiens
21 UCHL5 51377
Reconstituted Complex Homo sapiens
22 CEP57  
Affinity Capture-MS Homo sapiens
23 METTL23  
Affinity Capture-MS Homo sapiens
24 CYLD  
Affinity Capture-MS Homo sapiens
25 ASF1A 25842
Affinity Capture-MS Homo sapiens
26 USP53 54532
Affinity Capture-MS Homo sapiens
27 CTSV 1515
Affinity Capture-MS Homo sapiens
28 ZDHHC17 23390
Two-hybrid Homo sapiens
29 COX7A2 1347
Co-fractionation Homo sapiens
30 UBN2  
Affinity Capture-MS Homo sapiens
31 DSG1 1828
Affinity Capture-MS Homo sapiens
32 ARHGEF39 84904
Affinity Capture-MS Homo sapiens
33 CABIN1  
Affinity Capture-MS Homo sapiens
34 CUL4B 8450
Affinity Capture-MS Homo sapiens
35 CTSO  
Affinity Capture-MS Homo sapiens
36 RTCA 8634
Affinity Capture-MS Homo sapiens
37 ARG1 383
Affinity Capture-MS Homo sapiens
38 MSMB  
Affinity Capture-MS Homo sapiens
39 RASSF9  
Affinity Capture-MS Homo sapiens
40 RCCD1  
Affinity Capture-MS Homo sapiens
41 TUBA1B 10376
Affinity Capture-MS Homo sapiens
42 CTSC 1075
Affinity Capture-MS Homo sapiens
43 SLX4  
Affinity Capture-MS Homo sapiens
44 PIGT 51604
Affinity Capture-MS Homo sapiens
45 IFI16 3428
Affinity Capture-MS Homo sapiens
46 SCML2  
Affinity Capture-MS Homo sapiens
47 CTSB 1508
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
48 APTX  
Affinity Capture-MS Homo sapiens
49 DDX39B 7919
Affinity Capture-MS Homo sapiens
50 ARHGAP20  
Affinity Capture-MS Homo sapiens
51 VPS11 55823
Affinity Capture-MS Homo sapiens
52 IVL 3713
Reconstituted Complex Homo sapiens
53 FANCD2  
Affinity Capture-MS Homo sapiens
54 NASP 4678
Affinity Capture-MS Homo sapiens
55 EPAS1  
Affinity Capture-MS Homo sapiens
56 HRNR 388697
Affinity Capture-MS Homo sapiens
57 CD274 29126
Affinity Capture-MS Homo sapiens
58 S100A8 6279
Affinity Capture-MS Homo sapiens
59 PI3  
Reconstituted Complex Homo sapiens
60 EGFR 1956
Affinity Capture-MS Homo sapiens
61 LOR  
Reconstituted Complex Homo sapiens
62 VHL  
Affinity Capture-MS Homo sapiens
63 KDM3B  
Affinity Capture-MS Homo sapiens
64 CDK2 1017
Affinity Capture-MS Homo sapiens
65 FABP5 2171
Affinity Capture-MS Homo sapiens
66 ARHGAP4 393
Affinity Capture-MS Homo sapiens
67 RASSF10  
Affinity Capture-MS Homo sapiens
68 HIRA  
Affinity Capture-MS Homo sapiens
69 TCEB2 6923
Affinity Capture-MS Homo sapiens
70 CTSF 8722
Affinity Capture-MS Homo sapiens
71 HAT1 8520
Affinity Capture-MS Homo sapiens
72 DSP 1832
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
73 CDAN1 146059
Affinity Capture-MS Homo sapiens
74 PRMT3 10196
Affinity Capture-MS Homo sapiens
75 UBN1  
Affinity Capture-MS Homo sapiens
76 CTSL 1514
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
77 CUL2 8453
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 JUP 3728
Affinity Capture-MS Homo sapiens
79 TP53 7157
Affinity Capture-MS Homo sapiens
80 TGFBRAP1 9392
Affinity Capture-MS Homo sapiens
81 ASF1B  
Affinity Capture-MS Homo sapiens
82 PHF8  
Affinity Capture-MS Homo sapiens
83 KDM4C  
Affinity Capture-MS Homo sapiens
84 BAZ2A  
Affinity Capture-MS Homo sapiens
85 PLEKHG4B 153478
Affinity Capture-MS Homo sapiens
86 S100A9 6280
Affinity Capture-MS Homo sapiens
87 DCD 117159
Affinity Capture-MS Homo sapiens
88 CALML5 51806
Affinity Capture-MS Homo sapiens
89 SERPINB12 89777
Affinity Capture-MS Homo sapiens
90 RAD21 5885
Affinity Capture-Western Homo sapiens
91 PINK1  
Affinity Capture-MS Homo sapiens
92 TCF3  
Affinity Capture-MS Homo sapiens
93 METTL3  
Affinity Capture-MS Homo sapiens
94 FOXR1  
Affinity Capture-MS Homo sapiens
95 MOB1A 55233
Affinity Capture-MS Homo sapiens
96 ANXA2 302
Affinity Capture-MS Homo sapiens
97 CCR1  
Affinity Capture-MS Homo sapiens
98 CTSH 1512
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CSTA is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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