Gene description for CTSH
Gene name cathepsin H
Gene symbol CTSH
Other names/aliases ACC-4
ACC-5
ACC4
ACC5
CPSB
minichain
Species Homo sapiens
 Database cross references - CTSH
ExoCarta ExoCarta_1512
Vesiclepedia VP_1512
Entrez Gene 1512
HGNC 2535
MIM 116820
UniProt P09668  
 CTSH identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CTSH
Molecular Function
    endopeptidase activity GO:0004175 IDA
    aminopeptidase activity GO:0004177 IDA
    cysteine-type endopeptidase activity GO:0004197 IBA
    cysteine-type endopeptidase activity GO:0004197 IDA
    cysteine-type endopeptidase activity GO:0004197 TAS
    serine-type endopeptidase activity GO:0004252 ISS
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 IDA
    cysteine-type peptidase activity GO:0008234 IDA
    cysteine-type endopeptidase activator activity involved in apoptotic process GO:0008656 IBA
    cysteine-type endopeptidase activator activity involved in apoptotic process GO:0008656 IDA
    HLA-A specific activating MHC class I receptor activity GO:0030108 IDA
    thyroid hormone binding GO:0070324 IDA
Biological Process
    metanephros development GO:0001656 ISS
    T cell mediated cytotoxicity GO:0001913 ISS
    adaptive immune response GO:0002250 IEP
    immune response-regulating signaling pathway GO:0002764 IDA
    proteolysis GO:0006508 IDA
    proteolysis GO:0006508 TAS
    apoptotic process GO:0006915 IEA
    immune response GO:0006955 IBA
    positive regulation of cell population proliferation GO:0008284 ISS
    positive regulation of gene expression GO:0010628 IDA
    positive regulation of epithelial cell migration GO:0010634 ISS
    neuropeptide catabolic process GO:0010813 IDA
    bradykinin catabolic process GO:0010815 IDA
    positive regulation of peptidase activity GO:0010952 IDA
    antigen processing and presentation GO:0019882 TAS
    positive regulation of cell migration GO:0030335 IDA
    zymogen activation GO:0031638 IDA
    protein destabilization GO:0031648 IMP
    response to retinoic acid GO:0032526 ISS
    membrane protein proteolysis GO:0033619 IDA
    negative regulation of apoptotic process GO:0043066 ISS
    surfactant homeostasis GO:0043129 IDA
    positive regulation of angiogenesis GO:0045766 ISS
    proteolysis involved in protein catabolic process GO:0051603 IBA
    dichotomous subdivision of terminal units involved in lung branching GO:0060448 ISS
    ERK1 and ERK2 cascade GO:0070371 IDA
    cellular response to thyroid hormone stimulus GO:0097067 IEP
    positive regulation of apoptotic signaling pathway GO:2001235 IBA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    cytosol GO:0005829 IDA
    secretory granule lumen GO:0034774 TAS
    cytoplasmic ribonucleoprotein granule GO:0036464 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
    alveolar lamellar body GO:0097208 IDA
    multivesicular body lumen GO:0097486 TAS
    tertiary granule lumen GO:1904724 TAS
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified CTSH in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CTSH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRSS16 10279
Affinity Capture-MS Homo sapiens
2 CANX 821
Affinity Capture-MS Homo sapiens
3 IFNA4  
Affinity Capture-MS Homo sapiens
4 CST1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PRG2  
Affinity Capture-MS Homo sapiens
6 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
7 RHBDD1 84236
Affinity Capture-MS Homo sapiens
8 FAM153B  
Affinity Capture-MS Homo sapiens
9 CTSL 1514
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CCDC103  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 APTX  
Affinity Capture-MS Homo sapiens
12 CST5 1473
Affinity Capture-MS Homo sapiens
13 CCNC  
Affinity Capture-MS Homo sapiens
14 NT5C1A  
Affinity Capture-MS Homo sapiens
15 SLCO6A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 LDHA 3939
Co-fractionation Homo sapiens
17 TCTN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TRIM41 90933
Affinity Capture-MS Homo sapiens
20 CST3 1471
Affinity Capture-MS Homo sapiens
21 EMC4 51234
Affinity Capture-MS Homo sapiens
22 ZNF154  
Affinity Capture-MS Homo sapiens
23 PCP4  
Affinity Capture-MS Homo sapiens
24 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
25 CSTA 1475
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
26 ZIC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PTDSS1 9791
Affinity Capture-MS Homo sapiens
28 CSTB 1476
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
29 ST7  
Affinity Capture-MS Homo sapiens
30 MRM1  
Affinity Capture-MS Homo sapiens
31 XRN1 54464
Two-hybrid Homo sapiens
32 NONO 4841
Affinity Capture-MS Homo sapiens
33 SH2D3A  
Affinity Capture-MS Homo sapiens
34 SF3A1 10291
Affinity Capture-MS Homo sapiens
35 DNAJA2 10294
Affinity Capture-MS Homo sapiens
36 ERCC3  
Affinity Capture-MS Homo sapiens
37 HES4  
Affinity Capture-MS Homo sapiens
38 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 CREM  
Affinity Capture-MS Homo sapiens
40 TEFM  
Affinity Capture-MS Homo sapiens
41 SARAF  
Affinity Capture-MS Homo sapiens
42 AIRE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 TSPAN6 7105
Affinity Capture-MS Homo sapiens
44 IGF2R 3482
Affinity Capture-MS Homo sapiens
45 KNG1 3827
Affinity Capture-MS Homo sapiens
46 E2F6 1876
Affinity Capture-MS Homo sapiens
47 NUDT3 11165
Affinity Capture-MS Homo sapiens
48 HSPD1 3329
Affinity Capture-MS Homo sapiens
49 GABPA 2551
Affinity Capture-MS Homo sapiens
50 CCR1  
Affinity Capture-MS Homo sapiens
51 MMRN1 22915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 STX17 55014
Affinity Capture-MS Homo sapiens
53 PFDN5 5204
Affinity Capture-MS Homo sapiens
54 RIPPLY3  
Affinity Capture-MS Homo sapiens
55 POC5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 BPNT1 10380
Affinity Capture-MS Homo sapiens
57 VWA3B 200403
Affinity Capture-MS Homo sapiens
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