Gene description for EMC4
Gene name ER membrane protein complex subunit 4
Gene symbol EMC4
Other names/aliases PIG17
TMEM85
Species Homo sapiens
 Database cross references - EMC4
ExoCarta ExoCarta_51234
Vesiclepedia VP_51234
Entrez Gene 51234
HGNC 28032
MIM 616245
UniProt Q5J8M3  
 EMC4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for EMC4
Molecular Function
    protein binding GO:0005515 IPI
    membrane insertase activity GO:0032977 IMP
Biological Process
    apoptotic process GO:0006915 IEA
    protein insertion into ER membrane by stop-transfer membrane-anchor sequence GO:0045050 IDA
    protein insertion into ER membrane by stop-transfer membrane-anchor sequence GO:0045050 IMP
    tail-anchored membrane protein insertion into ER membrane GO:0071816 IDA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 NAS
    membrane GO:0016020 IDA
    EMC complex GO:0072546 IBA
    EMC complex GO:0072546 IDA
    EMC complex GO:0072546 IPI
    EMC complex GO:0072546 ISS
 Experiment description of studies that identified EMC4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for EMC4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 OR2M7  
Affinity Capture-MS Homo sapiens
2 RNF149 284996
Affinity Capture-MS Homo sapiens
3 RPS12 6206
Affinity Capture-MS Homo sapiens
4 HACD3 51495
Co-fractionation Homo sapiens
5 SLC25A13 10165
Co-fractionation Homo sapiens
6 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
7 TRIM3 10612
Affinity Capture-MS Homo sapiens
8 NDUFS1 4719
Co-fractionation Homo sapiens
9 RPL30 6156
Affinity Capture-MS Homo sapiens
10 OR1E1  
Affinity Capture-MS Homo sapiens
11 TPP1 1200
Co-fractionation Homo sapiens
12 RPA2 6118
Proximity Label-MS Homo sapiens
13 RPL13A 23521
Affinity Capture-MS Homo sapiens
14 TAC3  
Affinity Capture-MS Homo sapiens
15 HSD17B3  
Affinity Capture-MS Homo sapiens
16 DNAJC25 548645
Proximity Label-MS Homo sapiens
17 PTPN1 5770
Proximity Label-MS Homo sapiens
18 MUCL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CPT1A 1374
Co-fractionation Homo sapiens
20 RPL22 6146
Affinity Capture-MS Homo sapiens
21 LRPPRC 10128
Co-fractionation Homo sapiens
22 POR 5447
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
23 NDUFS3 4722
Co-fractionation Homo sapiens
24 Emc3  
Affinity Capture-MS Mus musculus
25 TRDN 10345
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 RPL24 6152
Affinity Capture-MS Homo sapiens
27 RPS8 6202
Affinity Capture-MS Homo sapiens
28 DDOST 1650
Affinity Capture-MS Homo sapiens
29 DNAJC16  
Proximity Label-MS Homo sapiens
30 CCDC47 57003
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 NDUFV1 4723
Co-fractionation Homo sapiens
32 EMC1 23065
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 EMC9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 NNT 23530
Co-fractionation Homo sapiens
35 RPS23 6228
Affinity Capture-MS Homo sapiens
36 RPS9 6203
Affinity Capture-MS Homo sapiens
37 SERPINB4 6318
Affinity Capture-MS Homo sapiens
38 S100A2 6273
Affinity Capture-MS Homo sapiens
39 EMC10 284361
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 P4HA1 5033
Co-fractionation Homo sapiens
41 TXNL4B  
Affinity Capture-MS Homo sapiens
42 OSBPL8 114882
Affinity Capture-MS Homo sapiens
43 DEFB106A  
Affinity Capture-MS Homo sapiens
44 CYB5A 1528
Affinity Capture-MS Homo sapiens
45 SREBF2 6721
Negative Genetic Homo sapiens
46 IGHG1 3500
Affinity Capture-MS Homo sapiens
47 RPL31 6160
Affinity Capture-MS Homo sapiens
48 IGHG4 3503
Affinity Capture-MS Homo sapiens
49 SLC28A2  
Affinity Capture-MS Homo sapiens
50 CYC1 1537
Co-fractionation Homo sapiens
51 UQCRC1 7384
Co-fractionation Homo sapiens
52 GNRH1  
Affinity Capture-MS Homo sapiens
53 FAM19A2 338811
Affinity Capture-MS Homo sapiens
54 RPLP0 6175
Affinity Capture-MS Homo sapiens
55 UQCRH 7388
Co-fractionation Homo sapiens
56 SERPINA3 12
Affinity Capture-MS Homo sapiens
57 SLC25A46 91137
Proximity Label-MS Homo sapiens
58 ATP5B 506
Co-fractionation Homo sapiens
59 RMDN3 55177
Proximity Label-MS Homo sapiens
60 RPL11 6135
Affinity Capture-MS Homo sapiens
61 CALML3 810
Affinity Capture-MS Homo sapiens
62 C7orf34  
Affinity Capture-MS Homo sapiens
63 SEC63 11231
Proximity Label-MS Homo sapiens
64 RPL32 6161
Affinity Capture-MS Homo sapiens
65 SERPINB3 6317
Affinity Capture-MS Homo sapiens
66 EMC3 55831
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 RPN2 6185
Affinity Capture-MS Homo sapiens
68 RPL10 6134
Affinity Capture-MS Homo sapiens
69 CANX 821
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
70 RAB7A 7879
Proximity Label-MS Homo sapiens
71 EMC8 10328
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 LRRC59 55379
Co-fractionation Homo sapiens
73 UQCRC2 7385
Co-fractionation Homo sapiens
74 HMOX2 3163
Co-fractionation Homo sapiens
75 Emc6  
Affinity Capture-MS Mus musculus
76 IGKC 3514
Affinity Capture-MS Homo sapiens
77 CRELD1 78987
Affinity Capture-MS Homo sapiens
78 S100A7 6278
Affinity Capture-MS Homo sapiens
79 EGFR 1956
Negative Genetic Homo sapiens
80 FKBP8 23770
Proximity Label-MS Homo sapiens
81 WFDC2  
Affinity Capture-MS Homo sapiens
82 S100A7A 338324
Affinity Capture-MS Homo sapiens
83 RPL7 6129
Affinity Capture-MS Homo sapiens
84 NXF1 10482
Affinity Capture-RNA Homo sapiens
85 GJB1 2705
Affinity Capture-Western Homo sapiens
86 LACRT 90070
Affinity Capture-MS Homo sapiens
87 RPA3 6119
Proximity Label-MS Homo sapiens
88 RPS13 6207
Affinity Capture-MS Homo sapiens
89 FBXO2 26232
Affinity Capture-MS Homo sapiens
90 EMC2 9694
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 ATP6V1A 523
Co-fractionation Homo sapiens
92 NCLN 56926
Affinity Capture-MS Homo sapiens
93 RPLP1 6176
Affinity Capture-MS Homo sapiens
94 IFNA21  
Affinity Capture-MS Homo sapiens
95 SCCPDH 51097
Co-fractionation Homo sapiens
96 RPS16 6217
Affinity Capture-MS Homo sapiens
97 EMC6 83460
Affinity Capture-MS Homo sapiens
98 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
99 PHB2 11331
Co-fractionation Homo sapiens
100 Emc1 230866
Affinity Capture-MS Mus musculus
101 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
102 SLC18A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 CHTOP  
Affinity Capture-MS Homo sapiens
104 SCGB1D1  
Affinity Capture-MS Homo sapiens
105 PGRMC1 10857
Affinity Capture-MS Homo sapiens
106 LONP1 9361
Co-fractionation Homo sapiens
107 EMC7 56851
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 APEX1 328
Affinity Capture-RNA Homo sapiens
109 SAA1 6288
Affinity Capture-MS Homo sapiens
110 OR1A2  
Affinity Capture-MS Homo sapiens
111 BCKDHA 593
Co-fractionation Homo sapiens
112 PDHA1 5160
Co-fractionation Homo sapiens
113 NDUFS7 374291
Co-fractionation Homo sapiens
114 PDHB 5162
Co-fractionation Homo sapiens
115 IGHG2 3501
Affinity Capture-MS Homo sapiens
116 ATP2A2 488
Affinity Capture-MS Homo sapiens
117 MMGT1 93380
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 EEF2 1938
Co-fractionation Homo sapiens
119 SCN4A  
Affinity Capture-MS Homo sapiens
120 NDUFS8 4728
Co-fractionation Homo sapiens
121 ADIPOQ 9370
Affinity Capture-MS Homo sapiens
122 MOV10 4343
Affinity Capture-RNA Homo sapiens
123 SPCS1 28972
Affinity Capture-MS Homo sapiens
124 GM2A 2760
Affinity Capture-MS Homo sapiens
125 CXCL6  
Affinity Capture-MS Homo sapiens
126 GPC3 2719
Affinity Capture-MS Homo sapiens
127 RPS7 6201
Affinity Capture-MS Homo sapiens
128 RPL28 6158
Affinity Capture-MS Homo sapiens
129 DNAJC1 64215
Proximity Label-MS Homo sapiens
130 LCN2 3934
Affinity Capture-MS Homo sapiens
131 PLAC9 219348
Affinity Capture-MS Homo sapiens
132 C12orf57 113246
Affinity Capture-MS Homo sapiens
133 DNAJC22  
Proximity Label-MS Homo sapiens
134 CTSH 1512
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which EMC4 is involved
No pathways found





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