Gene description for TRDN
Gene name triadin
Gene symbol TRDN
Other names/aliases CPVT5
TDN
TRISK
Species Homo sapiens
 Database cross references - TRDN
ExoCarta ExoCarta_10345
Vesiclepedia VP_10345
Entrez Gene 10345
HGNC 12261
MIM 603283
UniProt Q13061  
 TRDN identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TRDN
Molecular Function
    signaling receptor binding GO:0005102 IEA
    protein binding GO:0005515 IPI
    protein-macromolecule adaptor activity GO:0030674 IBA
    protein-macromolecule adaptor activity GO:0030674 ISS
    transmembrane transporter binding GO:0044325 ISS
Biological Process
    intracellular calcium ion homeostasis GO:0006874 ISS
    muscle contraction GO:0006936 TAS
    response to bacterium GO:0009617 IEA
    regulation of cell communication by electrical coupling GO:0010649 TAS
    regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0010880 IBA
    regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0010880 ISS
    release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0014808 IBA
    release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0014808 ISS
    cytoplasmic microtubule organization GO:0031122 ISS
    regulation of release of sequestered calcium ion into cytosol GO:0051279 TAS
    establishment of localization in cell GO:0051649 IEA
    heart contraction GO:0060047 IBA
    heart contraction GO:0060047 IMP
    negative regulation of ryanodine-sensitive calcium-release channel activity GO:0060315 TAS
    positive regulation of ryanodine-sensitive calcium-release channel activity GO:0060316 ISS
    positive regulation of ryanodine-sensitive calcium-release channel activity GO:0060316 TAS
    regulation of cardiac muscle cell membrane potential GO:0086036 IBA
    regulation of cardiac muscle cell membrane potential GO:0086036 ISS
    endoplasmic reticulum membrane organization GO:0090158 ISS
    positive regulation of cell communication by electrical coupling involved in cardiac conduction GO:1901846 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    endoplasmic reticulum GO:0005783 ISS
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    junctional sarcoplasmic reticulum membrane GO:0014701 IBA
    junctional sarcoplasmic reticulum membrane GO:0014701 ISS
    junctional sarcoplasmic reticulum membrane GO:0014701 TAS
    membrane GO:0016020 TAS
    sarcoplasmic reticulum GO:0016529 ISS
    junctional membrane complex GO:0030314 ISS
    sarcoplasmic reticulum membrane GO:0033017 TAS
    sarcoplasmic reticulum lumen GO:0033018 TAS
 Experiment description of studies that identified TRDN in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TRDN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RHOT1 55288
Affinity Capture-MS Homo sapiens
2 EMC10 284361
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 BET1 10282
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MANEA 79694
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SEC63 11231
Affinity Capture-MS Homo sapiens
6 PXMP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TMEM106B 54664
Affinity Capture-MS Homo sapiens
8 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 EMC3 55831
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PDIA5 10954
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ZFPL1 7542
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 LMAN2L 81562
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ABCC5 10057
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ATAD3B 83858
Affinity Capture-MS Homo sapiens
16 PNN 5411
Cross-Linking-MS (XL-MS) Homo sapiens
17 GOLIM4 27333
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TMEM223  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 EMC7 56851
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 RPS4X 6191
Affinity Capture-MS Homo sapiens
21 JPH1 56704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 TMEM205 374882
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PPIB 5479
Two-hybrid Homo sapiens
24 EMC4 51234
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 MPV17L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 NR3C1 2908
Proximity Label-MS Homo sapiens
27 EFNB1 1947
Affinity Capture-MS Homo sapiens
28 RPL21 6144
Cross-Linking-MS (XL-MS) Homo sapiens
29 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
30 EMC1 23065
Affinity Capture-MS Homo sapiens
31 CALM1 801
Reconstituted Complex Homo sapiens
32 EXOG 9941
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
34 PRG4 10216
Cross-Linking-MS (XL-MS) Homo sapiens
35 HSP90AA1 3320
Cross-Linking-MS (XL-MS) Homo sapiens
36 NAT14 57106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 GOSR2 9570
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ANKH 56172
Affinity Capture-MS Homo sapiens
39 LRP10 26020
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 KIAA1467 57613
Affinity Capture-MS Homo sapiens
41 RYR1 6261
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
42 HRC  
Reconstituted Complex Homo sapiens
43 XRCC5 7520
Cross-Linking-MS (XL-MS) Homo sapiens
44 GP1BB 2812
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 TTN 7273
Cross-Linking-MS (XL-MS) Homo sapiens
46 EMC2 9694
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 RPL7A 6130
Cross-Linking-MS (XL-MS) Homo sapiens
48 CYB5R3 1727
Cross-Linking-MS (XL-MS) Homo sapiens
49 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
50 KCNN4 3783
Affinity Capture-MS Homo sapiens
51 TMEM9 252839
Affinity Capture-MS Homo sapiens
52 STX8 9482
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 TMEM9B 56674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 ABCB10 23456
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 DNAJC3 5611
Cross-Linking-MS (XL-MS) Homo sapiens
56 EMC8 10328
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 PCYOX1 51449
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 RMDN3 55177
Affinity Capture-MS Homo sapiens
59 EMC9  
Affinity Capture-MS Homo sapiens
60 SCAMP3 10067
Affinity Capture-MS Homo sapiens
61 KCNE4  
Two-hybrid Homo sapiens
62 UGT8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 TMEM192 201931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 CASQ2 845
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
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