Gene description for GABPA
Gene name GA binding protein transcription factor, alpha subunit 60kDa
Gene symbol GABPA
Other names/aliases E4TF1-60
E4TF1A
NFT2
NRF2
NRF2A
RCH04A07
Species Homo sapiens
 Database cross references - GABPA
ExoCarta ExoCarta_2551
Vesiclepedia VP_2551
Entrez Gene 2551
HGNC 4071
MIM 600609
UniProt Q06546  
 GABPA identified in sEVs derived from the following tissue/cell type
Keratinocytes 19530224    
Keratinocytes 19530224    
 Gene ontology annotations for GABPA
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    chromatin binding GO:0003682 IEA
    DNA-binding transcription factor activity GO:0003700 TAS
    protein binding GO:0005515 IPI
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    blastocyst formation GO:0001825 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    cell differentiation GO:0030154 IBA
    negative regulation of megakaryocyte differentiation GO:0045653 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
Subcellular Localization
    chromatin GO:0000785 IDA
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified GABPA in sEVs
1
Experiment ID 189
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 190
MISEV standards
EM
Biophysical techniques
HSC70
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GABPA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CAPNS2 84290
Affinity Capture-MS Homo sapiens
2 SERPINB4 6318
Affinity Capture-MS Homo sapiens
3 ELF1 1997
Proximity Label-MS Homo sapiens
4 FEV  
Proximity Label-MS Homo sapiens
5 KPNA1 3836
Co-fractionation Homo sapiens
6 CALML3 810
Affinity Capture-MS Homo sapiens
7 KLF9 687
Proximity Label-MS Homo sapiens
8 TPD52L2 7165
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ANXA3 306
Affinity Capture-MS Homo sapiens
10 IL36G 56300
Affinity Capture-MS Homo sapiens
11 ZNF200  
Affinity Capture-MS Homo sapiens
12 SP1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 NUP62 23636
Cross-Linking-MS (XL-MS) Homo sapiens
14 LIMCH1 22998
Co-fractionation Homo sapiens
15 GDPD3 79153
Affinity Capture-MS Homo sapiens
16 GNE 10020
Co-fractionation Homo sapiens
17 ALOX12B  
Affinity Capture-MS Homo sapiens
18 ELF4 2000
Proximity Label-MS Homo sapiens
19 APP 351
Reconstituted Complex Homo sapiens
20 APOD 347
Affinity Capture-MS Homo sapiens
21 S100A14 57402
Affinity Capture-MS Homo sapiens
22 GSDMA 284110
Affinity Capture-MS Homo sapiens
23 HSPA2 3306
Affinity Capture-MS Homo sapiens
24 KLF8  
Proximity Label-MS Homo sapiens
25 S100P 6286
Affinity Capture-MS Homo sapiens
26 GABPB2  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
27 ACPP 55
Affinity Capture-MS Homo sapiens
28 ATF1  
Reconstituted Complex Homo sapiens
29 NCOA7  
Co-fractionation Homo sapiens
30 KPRP 448834
Affinity Capture-MS Homo sapiens
31 LHX4  
Proximity Label-MS Homo sapiens
32 PAPL  
Affinity Capture-MS Homo sapiens
33 GBX1  
Affinity Capture-MS Homo sapiens
34 CTSV 1515
Affinity Capture-MS Homo sapiens
35 MED1 5469
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
36 KLF16  
Proximity Label-MS Homo sapiens
37 ALDH1A3 220
Affinity Capture-MS Homo sapiens
38 S100A2 6273
Affinity Capture-MS Homo sapiens
39 POF1B 79983
Affinity Capture-MS Homo sapiens
40 CBX3 11335
Proximity Label-MS Homo sapiens
41 PINX1  
Two-hybrid Homo sapiens
42 PARK2  
Affinity Capture-MS Homo sapiens
43 CST6  
Affinity Capture-MS Homo sapiens
44 NFIX 4784
Proximity Label-MS Homo sapiens
45 DIABLO 56616
Co-fractionation Homo sapiens
46 C22orf15  
Affinity Capture-MS Homo sapiens
47 IGHG1 3500
Affinity Capture-MS Homo sapiens
48 OGT 8473
Reconstituted Complex Homo sapiens
49 LHX3  
Proximity Label-MS Homo sapiens
50 S100A8 6279
Affinity Capture-MS Homo sapiens
51 CPA4 51200
Affinity Capture-MS Homo sapiens
52 SMPD3  
Affinity Capture-MS Homo sapiens
53 EVPL 2125
Affinity Capture-MS Homo sapiens
54 RAB3GAP1 22930
Co-fractionation Homo sapiens
55 TGM1 7051
Affinity Capture-MS Homo sapiens
56 SERPINB7 8710
Affinity Capture-MS Homo sapiens
57 SERPINA3 12
Affinity Capture-MS Homo sapiens
58 KLF12  
Proximity Label-MS Homo sapiens
59 CEBPA  
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
60 CD3EAP  
Proximity Label-MS Homo sapiens
61 SERPINA9 327657
Affinity Capture-MS Homo sapiens
62 YEATS4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 SPRR1A  
Affinity Capture-MS Homo sapiens
64 SERPINB3 6317
Affinity Capture-MS Homo sapiens
65 IVL 3713
Affinity Capture-MS Homo sapiens
66 SERPINB13 5275
Affinity Capture-MS Homo sapiens
67 TYMP 1890
Affinity Capture-MS Homo sapiens
68 ELK3  
Proximity Label-MS Homo sapiens
69 KCTD12 115207
Co-fractionation Homo sapiens
70 HCFC1 3054
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
71 IGKC 3514
Affinity Capture-MS Homo sapiens
72 KLK6 5653
Affinity Capture-MS Homo sapiens
73 S100A7 6278
Affinity Capture-MS Homo sapiens
74 EGFR 1956
Negative Genetic Homo sapiens
75 S100A7A 338324
Affinity Capture-MS Homo sapiens
76 GPRASP2  
Affinity Capture-MS Homo sapiens
77 A2ML1 144568
Affinity Capture-MS Homo sapiens
78 TPD52L3  
Affinity Capture-MS Homo sapiens
79 RPA3 6119
Proximity Label-MS Homo sapiens
80 TSEN34 79042
Co-fractionation Homo sapiens
81 SPRR1B 6699
Affinity Capture-MS Homo sapiens
82 GABPB1  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
83 ETV3  
Proximity Label-MS Homo sapiens
84 TCEB2 6923
Affinity Capture-MS Homo sapiens
85 CUTC  
Co-fractionation Homo sapiens
86 CORO6  
Affinity Capture-MS Homo sapiens
87 FHL2 2274
Affinity Capture-MS Homo sapiens
88 PLA2G4E  
Affinity Capture-MS Homo sapiens
89 STK16 8576
Two-hybrid Homo sapiens
90 RAB3GAP2 25782
Co-fractionation Homo sapiens
91 JUP 3728
Affinity Capture-MS Homo sapiens
92 AZGP1 563
Affinity Capture-MS Homo sapiens
93 FOXJ2  
Affinity Capture-MS Homo sapiens
94 PIN1 5300
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
95 CRABP2 1382
Affinity Capture-MS Homo sapiens
96 KEAP1 9817
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
97 HDAC1 3065
Affinity Capture-Western Homo sapiens
98 KLK7 5650
Affinity Capture-MS Homo sapiens
99 S100A9 6280
Affinity Capture-MS Homo sapiens
100 PKP1 5317
Affinity Capture-MS Homo sapiens
101 ZC2HC1B  
Two-hybrid Homo sapiens
102 IGLL5 100423062
Affinity Capture-MS Homo sapiens
103 GM2A 2760
Affinity Capture-MS Homo sapiens
104 CTSH 1512
Affinity Capture-MS Homo sapiens
105 PLA2G4D  
Affinity Capture-MS Homo sapiens
106 NCCRP1 342897
Affinity Capture-MS Homo sapiens
107 ELF2  
Proximity Label-MS Homo sapiens
108 KRAS 3845
Synthetic Lethality Homo sapiens
109 LCN2 3934
Affinity Capture-MS Homo sapiens
110 FCGBP 8857
Affinity Capture-MS Homo sapiens
111 SP7  
Proximity Label-MS Homo sapiens
112 SULT2B1 6820
Affinity Capture-MS Homo sapiens
113 EP300 2033
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
114 SP3  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
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