Gene description for CAPNS2
Gene name calpain, small subunit 2
Gene symbol CAPNS2
Other names/aliases -
Species Homo sapiens
 Database cross references - CAPNS2
ExoCarta ExoCarta_84290
Vesiclepedia VP_84290
Entrez Gene 84290
HGNC 16371
UniProt Q96L46  
 CAPNS2 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for CAPNS2
Molecular Function
    calcium-dependent cysteine-type endopeptidase activity GO:0004198 IDA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    calpain complex GO:0110158 IBA
 Experiment description of studies that identified CAPNS2 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CAPNS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRSS16 10279
Affinity Capture-MS Homo sapiens
2 LIX1L 128077
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 PPIC 5480
Two-hybrid Homo sapiens
4 FAM153B  
Affinity Capture-MS Homo sapiens
5 SLC25A6 293
Affinity Capture-MS Homo sapiens
6 CCDC103  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 NDUFS5 4725
Affinity Capture-MS Homo sapiens
8 PINK1  
Affinity Capture-MS Homo sapiens
9 RPS19BP1  
Affinity Capture-MS Homo sapiens
10 SH2D3A  
Affinity Capture-MS Homo sapiens
11 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ACTA2 59
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 SRP9 6726
Affinity Capture-MS Homo sapiens
14 ZNF154  
Affinity Capture-MS Homo sapiens
15 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
16 OXNAD1 92106
Two-hybrid Homo sapiens
17 NPPA  
Affinity Capture-MS Homo sapiens
18 ST6GALNAC6 30815
Affinity Capture-MS Homo sapiens
19 PTDSS1 9791
Affinity Capture-MS Homo sapiens
20 FTH1 2495
Affinity Capture-MS Homo sapiens
21 ZIC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 FNDC5  
Affinity Capture-MS Homo sapiens
23 KLHL11  
Affinity Capture-MS Homo sapiens
24 CAST 831
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 RBFOX1 54715
Affinity Capture-MS Homo sapiens
26 SSUH2  
Affinity Capture-MS Homo sapiens
27 TOMM6  
Affinity Capture-MS Homo sapiens
28 RBM42  
Affinity Capture-MS Homo sapiens
29 TEFM  
Affinity Capture-MS Homo sapiens
30 SARAF  
Affinity Capture-MS Homo sapiens
31 CAPNS1 826
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CMTM5  
Two-hybrid Homo sapiens
33 RIN1 9610
Two-hybrid Homo sapiens
34 GOT1 2805
Affinity Capture-MS Homo sapiens
35 E2F6 1876
Affinity Capture-MS Homo sapiens
36 GLYR1 84656
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 NUDT3 11165
Affinity Capture-MS Homo sapiens
38 OR2A4 79541
Affinity Capture-MS Homo sapiens
39 GMCL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 UPK1A 11045
Affinity Capture-MS Homo sapiens
41 CAPN1 823
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 GABPA 2551
Affinity Capture-MS Homo sapiens
43 CCR1  
Affinity Capture-MS Homo sapiens
44 ACTBL2 345651
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ARMC10 83787
Two-hybrid Homo sapiens
46 PRPF31 26121
Two-hybrid Homo sapiens
47 APP 351
Reconstituted Complex Homo sapiens
48 THAP10  
Affinity Capture-MS Homo sapiens
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