Experiment description of studies that identified LIMCH1 in sEVs

1 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for LIMCH1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 LRIG3   Two-hybrid Homo sapiens 2 MAP3K6   Affinity Capture-MS Homo sapiens 3 ANLN 54443 Affinity Capture-MS Homo sapiens 4 TRMT61A 115708 Co-fractionation Homo sapiens 5 PHLPP1   Proximity Label-MS Homo sapiens 6 ISG15 9636 Affinity Capture-MS Homo sapiens 7 KLHL15   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 IFNG 3458 Affinity Capture-MS Homo sapiens 9 PPP1CB 5500 Affinity Capture-MS Homo sapiens 10 GNB4 59345 Affinity Capture-MS Homo sapiens 11 CUTC   Co-fractionation Homo sapiens 12 Myh9 17886 Affinity Capture-MS Mus musculus 13 FBXL6   Proximity Label-MS Homo sapiens 14 KPNA1 3836 Co-fractionation Homo sapiens 15 ABRA   Affinity Capture-MS Homo sapiens 16 XPO1 7514 Affinity Capture-MS Homo sapiens 17 NAGK 55577 Affinity Capture-MS Homo sapiens 18 UNK   Affinity Capture-RNA Homo sapiens 19 PFN1 5216 Proximity Label-MS Homo sapiens 20 RPA2 6118 Affinity Capture-MS Homo sapiens 21 CTNNB1 1499 Affinity Capture-MS Homo sapiens 22 Calml3   Affinity Capture-MS Mus musculus 23 RPA1 6117 Affinity Capture-MS Homo sapiens 24 Myo1c 17913 Affinity Capture-MS Mus musculus 25 MYO18A 399687 Affinity Capture-MS Homo sapiens 26 Itga5 16402 Affinity Capture-MS Mus musculus 27 CAPZA2 830 Affinity Capture-MS Homo sapiens 28 BTF3 689 Affinity Capture-MS Homo sapiens 29 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 30 MYO19   Affinity Capture-MS Homo sapiens 31 Actb 11461 Affinity Capture-MS Mus musculus 32 OTUD6B 51633 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 APP 351 Reconstituted Complex Homo sapiens 34 MAGEE1   Affinity Capture-MS Homo sapiens 35 Myh10 77579 Affinity Capture-MS Mus musculus 36 APEX1 328 Affinity Capture-RNA Homo sapiens 37 YES1 7525 Affinity Capture-MS Homo sapiens 38 DIABLO 56616 Co-fractionation Homo sapiens 39 CDK2 1017 Affinity Capture-MS Homo sapiens 40 SYTL5 94122 Affinity Capture-MS Homo sapiens 41 CDH1 999 Proximity Label-MS Homo sapiens 42 MYC   Affinity Capture-MS Homo sapiens 43 RPA3 6119 Affinity Capture-MS Homo sapiens 44 MYH9 4627 Affinity Capture-MS Homo sapiens 45 DYRK1A 1859 Affinity Capture-MS Homo sapiens 46 SLC43A3 29015 Affinity Capture-MS Homo sapiens 47 NTRK1 4914 Affinity Capture-MS Homo sapiens 48 PINK1   Affinity Capture-MS Homo sapiens 49 STK38L 23012 Affinity Capture-MS Homo sapiens 50 PEX14 5195 Proximity Label-MS Homo sapiens 51 HIST1H1C 3006 Cross-Linking-MS (XL-MS) Homo sapiens 52 IQGAP1 8826 Affinity Capture-MS Homo sapiens 53 ACTB 60 Proximity Label-MS Homo sapiens 54 NPM1 4869 Co-fractionation Homo sapiens 55 SH3KBP1 30011 Affinity Capture-MS Homo sapiens 56 AR 367 Affinity Capture-MS Homo sapiens 57 RFC2 5982 Affinity Capture-MS Homo sapiens 58 NAA40   Affinity Capture-MS Homo sapiens 59 BIRC8   Affinity Capture-MS Homo sapiens 60 DBN1 1627 Cross-Linking-MS (XL-MS) Homo sapiens 61 GABPA 2551 Co-fractionation Homo sapiens 62 EZR 7430 Proximity Label-MS Homo sapiens 63 TNNI2 7136 Affinity Capture-MS Homo sapiens 64 HUWE1 10075 Co-fractionation Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 65 Kif3c   Affinity Capture-MS Mus musculus 66 FRMPD2   Affinity Capture-MS Homo sapiens View the network

Pathways in which LIMCH1 is involved

No pathways found