Gene description for RFC2
Gene name replication factor C (activator 1) 2, 40kDa
Gene symbol RFC2
Other names/aliases RFC40
Species Homo sapiens
 Database cross references - RFC2
ExoCarta ExoCarta_5982
Vesiclepedia VP_5982
Entrez Gene 5982
HGNC 9970
MIM 600404
UniProt P35250  
 RFC2 identified in sEVs derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for RFC2
Molecular Function
    DNA binding GO:0003677 IEA
    DNA clamp loader activity GO:0003689 IBA
    DNA clamp loader activity GO:0003689 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    single-stranded DNA helicase activity GO:0017116 IDA
    enzyme binding GO:0019899 IEA
Biological Process
    DNA-templated DNA replication GO:0006261 IBA
    DNA-templated DNA replication GO:0006261 IDA
    DNA repair GO:0006281 IBA
    DNA duplex unwinding GO:0032508 IEA
    positive regulation of DNA-directed DNA polymerase activity GO:1900264 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    DNA replication factor C complex GO:0005663 IBA
    DNA replication factor C complex GO:0005663 IDA
    DNA replication factor C complex GO:0005663 IPI
    Ctf18 RFC-like complex GO:0031390 IDA
 Experiment description of studies that identified RFC2 in sEVs
1
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RFC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 SLFN11 91607
Affinity Capture-MS Homo sapiens
3 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 GTF2F1 2962
Affinity Capture-MS Homo sapiens
6 SPRTN  
Affinity Capture-MS Homo sapiens
7 PAXIP1  
Affinity Capture-MS Homo sapiens
8 PMS1  
Affinity Capture-Western Homo sapiens
9 RPA2 6118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
10 NFATC1 4772
Affinity Capture-MS Homo sapiens
11 LIMCH1 22998
Affinity Capture-MS Homo sapiens
12 NOP56 10528
Affinity Capture-MS Homo sapiens
13 RPL10 6134
Affinity Capture-MS Homo sapiens
14 C9orf78 51759
Affinity Capture-MS Homo sapiens
15 BRD2  
Affinity Capture-MS Homo sapiens
16 UBC 7316
Affinity Capture-MS Homo sapiens
17 CAND1 55832
Affinity Capture-MS Homo sapiens
18 SOX2  
Affinity Capture-MS Homo sapiens
19 CENPA  
Proximity Label-MS Homo sapiens
20 APEX1 328
Affinity Capture-RNA Homo sapiens
21 COPS5 10987
Affinity Capture-MS Homo sapiens
22 RFC3 5983
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
23 NPM1 4869
Affinity Capture-MS Homo sapiens
24 MDC1  
Affinity Capture-MS Homo sapiens
25 PSMC6 5706
Co-fractionation Homo sapiens
26 DDX1 1653
Co-fractionation Homo sapiens
27 HMGA1 3159
Affinity Capture-MS Homo sapiens
28 Set 56086
Affinity Capture-MS Mus musculus
29 BRD4 23476
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PMS2  
Affinity Capture-MS Homo sapiens
31 CUL1 8454
Affinity Capture-MS Homo sapiens
32 PSMC3 5702
Co-fractionation Homo sapiens
33 KIF14 9928
Affinity Capture-MS Homo sapiens
34 E2F4  
Affinity Capture-MS Homo sapiens
35 RAD23B 5887
Affinity Capture-MS Homo sapiens
36 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
37 RFC5 5985
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
38 PARK2  
Affinity Capture-MS Homo sapiens
39 RAD9A  
Affinity Capture-MS Homo sapiens
40 Srp72  
Affinity Capture-MS Mus musculus
41 LRRFIP1 9208
Co-fractionation Homo sapiens
42 BARD1 580
Affinity Capture-MS Homo sapiens
43 RAD1  
Co-fractionation Homo sapiens
44 IFI16 3428
Affinity Capture-MS Homo sapiens
45 RAD18  
Biochemical Activity Homo sapiens
46 HUWE1 10075
Affinity Capture-MS Homo sapiens
47 RFC4 5984
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Co-purification Homo sapiens
Co-fractionation Homo sapiens
48 Pcna 18538
Affinity Capture-MS Mus musculus
49 PRKDC 5591
Co-fractionation Homo sapiens
50 HECTD1 25831
Affinity Capture-MS Homo sapiens
51 CEP76  
Affinity Capture-MS Homo sapiens
52 SETD3 84193
Affinity Capture-MS Homo sapiens
53 CEBPA  
Protein-peptide Homo sapiens
54 DAXX  
Affinity Capture-MS Homo sapiens
55 BRCA1 672
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 CD3EAP  
Proximity Label-MS Homo sapiens
57 Brwd3  
Affinity Capture-MS Mus musculus
58 C16orf72 29035
Affinity Capture-MS Homo sapiens
59 NT5E 4907
Affinity Capture-MS Homo sapiens
60 HUS1  
Affinity Capture-MS Homo sapiens
61 FANCD2  
Affinity Capture-MS Homo sapiens
62 SKP2  
Affinity Capture-MS Homo sapiens
63 SMARCAD1  
Affinity Capture-MS Homo sapiens
64 TP53BP1 7158
Affinity Capture-MS Homo sapiens
65 UBE2H 7328
Affinity Capture-MS Homo sapiens
66 ECT2 1894
Affinity Capture-MS Homo sapiens
67 CUL3 8452
Affinity Capture-MS Homo sapiens
68 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
69 RFC1 5981
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
70 GINS3  
Co-fractionation Homo sapiens
71 MYC  
Affinity Capture-MS Homo sapiens
72 RPA3 6119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
73 FLII 2314
Co-fractionation Homo sapiens
74 DDRGK1 65992
Affinity Capture-MS Homo sapiens
75 Rpl35 66489
Affinity Capture-MS Mus musculus
76 Eif3e 16341
Affinity Capture-MS Mus musculus
77 LMNA 4000
Proximity Label-MS Homo sapiens
78 NFATC2  
Affinity Capture-MS Homo sapiens
79 BRD3 8019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 CCDC8  
Affinity Capture-MS Homo sapiens
81 RPS16 6217
Affinity Capture-MS Homo sapiens
82 HIF1A 3091
Affinity Capture-MS Homo sapiens
83 KRT18 3875
Co-fractionation Homo sapiens
84 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
85 PARP1 142
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 RAD17  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 DSCC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 CHTF18 63922
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
89 RPA1 6117
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 HLTF  
Affinity Capture-MS Homo sapiens
91 CHD1L 9557
Affinity Capture-MS Homo sapiens
92 SSBP1 6742
Affinity Capture-MS Homo sapiens
93 Prpf3 70767
Affinity Capture-MS Mus musculus
94 PCNA 5111
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 DDX11 1663
Affinity Capture-Western Homo sapiens
96 RTCB 51493
Co-fractionation Homo sapiens
97 C14orf166 51637
Co-fractionation Homo sapiens
98 SNW1 22938
Affinity Capture-MS Homo sapiens
99 KRAS 3845
Negative Genetic Homo sapiens
100 HNRNPU 3192
Affinity Capture-MS Homo sapiens
101 C9orf72  
Affinity Capture-MS Homo sapiens
102 SSRP1 6749
Affinity Capture-MS Homo sapiens
103 PURA 5813
Co-fractionation Homo sapiens
104 CCT8L2  
Affinity Capture-MS Homo sapiens
105 EXOSC1 51013
Affinity Capture-MS Homo sapiens
106 USP20  
Affinity Capture-MS Homo sapiens
107 EP300 2033
Affinity Capture-MS Homo sapiens
108 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RFC2 is involved
PathwayEvidenceSource
Activation of ATR in response to replication stress TAS Reactome
Base Excision Repair TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Chromosome Maintenance TAS Reactome
Defective homologous recombination repair (HRR) due to BRCA2 loss of function TAS Reactome
Disease TAS Reactome
Diseases of DNA Double-Strand Break Repair TAS Reactome
Diseases of DNA repair TAS Reactome
DNA Damage Bypass TAS Reactome
DNA Double-Strand Break Repair TAS Reactome
DNA Repair TAS Reactome
DNA Replication TAS Reactome
DNA strand elongation TAS Reactome
Dual Incision in GG-NER TAS Reactome
Dual incision in TC-NER TAS Reactome
Extension of Telomeres TAS Reactome
G2/M Checkpoints TAS Reactome
G2/M DNA damage checkpoint TAS Reactome
Gap-filling DNA repair synthesis and ligation in GG-NER TAS Reactome
Gap-filling DNA repair synthesis and ligation in TC-NER TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Global Genome Nucleotide Excision Repair (GG-NER) TAS Reactome
HDR through Homologous Recombination (HRR) TAS Reactome
HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) TAS Reactome
HDR through Single Strand Annealing (SSA) TAS Reactome
Homologous DNA Pairing and Strand Exchange TAS Reactome
Homology Directed Repair TAS Reactome
Impaired BRCA2 binding to RAD51 TAS Reactome
Lagging Strand Synthesis TAS Reactome
Leading Strand Synthesis TAS Reactome
Nucleotide Excision Repair TAS Reactome
PCNA-Dependent Long Patch Base Excision Repair TAS Reactome
Polymerase switching TAS Reactome
Polymerase switching on the C-strand of the telomere TAS Reactome
Presynaptic phase of homologous DNA pairing and strand exchange TAS Reactome
Processing of DNA double-strand break ends TAS Reactome
Recognition of DNA damage by PCNA-containing replication complex TAS Reactome
Regulation of TP53 Activity TAS Reactome
Regulation of TP53 Activity through Phosphorylation TAS Reactome
Resolution of Abasic Sites (AP sites) TAS Reactome
Resolution of AP sites via the multiple-nucleotide patch replacement pathway TAS Reactome
RNA Polymerase II Transcription TAS Reactome
S Phase TAS Reactome
Synthesis of DNA TAS Reactome
Telomere C-strand (Lagging Strand) Synthesis TAS Reactome
Telomere Maintenance TAS Reactome
Termination of translesion DNA synthesis TAS Reactome
Transcription-Coupled Nucleotide Excision Repair (TC-NER) TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
Translesion Synthesis by POLH TAS Reactome
Translesion synthesis by POLI TAS Reactome
Translesion synthesis by POLK TAS Reactome
Translesion synthesis by REV1 TAS Reactome
Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template TAS Reactome





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