Gene description for RFC3
Gene name replication factor C (activator 1) 3, 38kDa
Gene symbol RFC3
Other names/aliases RFC38
Species Homo sapiens
 Database cross references - RFC3
ExoCarta ExoCarta_5983
Vesiclepedia VP_5983
Entrez Gene 5983
HGNC 9971
MIM 600405
UniProt P40938  
 RFC3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for RFC3
Molecular Function
    DNA binding GO:0003677 IDA
    DNA clamp loader activity GO:0003689 IBA
    DNA clamp loader activity GO:0003689 IDA
    DNA clamp loader activity GO:0003689 TAS
    protein binding GO:0005515 IPI
    ATP-dependent activity, acting on DNA GO:0008094 IDA
    ATP hydrolysis activity GO:0016887 IEA
    single-stranded DNA helicase activity GO:0017116 IDA
Biological Process
    DNA synthesis involved in DNA repair GO:0000731 TAS
    DNA replication GO:0006260 IDA
    DNA-templated DNA replication GO:0006261 IBA
    DNA-templated DNA replication GO:0006261 IDA
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    DNA repair GO:0006281 IBA
    DNA duplex unwinding GO:0032508 IEA
    response to organophosphorus GO:0046683 IEP
    positive regulation of DNA-directed DNA polymerase activity GO:1900264 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    DNA replication factor C complex GO:0005663 IBA
    DNA replication factor C complex GO:0005663 IDA
    DNA replication factor C complex GO:0005663 IPI
    DNA replication factor C complex GO:0005663 TAS
    Ctf18 RFC-like complex GO:0031390 IDA
 Experiment description of studies that identified RFC3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RFC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FAN1  
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 MCM3 4172
Co-fractionation Homo sapiens
5 KHDRBS3  
Two-hybrid Homo sapiens
6 SPRTN  
Affinity Capture-MS Homo sapiens
7 HCST  
Affinity Capture-MS Homo sapiens
8 RPA2 6118
Affinity Capture-MS Homo sapiens
9 EBNA-LP  
Affinity Capture-MS
10 NOP56 10528
Affinity Capture-MS Homo sapiens
11 RPL10 6134
Affinity Capture-MS Homo sapiens
12 BRD2  
Affinity Capture-MS Homo sapiens
13 UBC 7316
Affinity Capture-MS Homo sapiens
14 PARK2  
Affinity Capture-MS Homo sapiens
15 D2HGDH 728294
Affinity Capture-MS Homo sapiens
16 RFXANK  
Affinity Capture-MS Homo sapiens
17 NPM1 4869
Affinity Capture-MS Homo sapiens
18 ATAD5  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
19 TULP3 7289
Affinity Capture-MS Homo sapiens
20 BRD4 23476
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
21 ATG16L1 55054
Affinity Capture-MS Homo sapiens
22 KIF14 9928
Affinity Capture-MS Homo sapiens
23 Cebpb  
Affinity Capture-Western Rattus norvegicus
24 NF2 4771
Affinity Capture-MS Homo sapiens
25 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
26 ANKRD40 91369
Two-hybrid Homo sapiens
27 RFC5 5985
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
28 CTDP1 9150
Affinity Capture-MS Homo sapiens
29 MEPCE 56257
Affinity Capture-MS Homo sapiens
30 ALPI  
Affinity Capture-MS Homo sapiens
31 RAD9A  
Affinity Capture-MS Homo sapiens
32 Srp72  
Affinity Capture-MS Mus musculus
33 CHMP4B 128866
Affinity Capture-MS Homo sapiens
34 BTF3 689
Affinity Capture-MS Homo sapiens
35 VCP 7415
Affinity Capture-MS Homo sapiens
36 NTRK1 4914
Affinity Capture-MS Homo sapiens
37 RAD1  
Affinity Capture-MS Homo sapiens
38 GPR182  
Affinity Capture-MS Homo sapiens
39 PARP1 142
Affinity Capture-MS Homo sapiens
40 HUWE1 10075
Affinity Capture-MS Homo sapiens
41 RFC4 5984
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
42 HECTD1 25831
Affinity Capture-MS Homo sapiens
43 Ksr1  
Affinity Capture-MS Mus musculus
44 RAD21 5885
Affinity Capture-Western Homo sapiens
45 DAXX  
Affinity Capture-MS Homo sapiens
46 UBXN6 80700
Affinity Capture-MS Homo sapiens
47 PURB 5814
Co-fractionation Homo sapiens
48 DNAJC5B  
Affinity Capture-MS Homo sapiens
49 MESDC2 23184
Two-hybrid Homo sapiens
50 Brwd3  
Affinity Capture-MS Mus musculus
51 FANCD2  
Affinity Capture-MS Homo sapiens
52 SMARCAD1  
Affinity Capture-MS Homo sapiens
53 ECT2 1894
Affinity Capture-MS Homo sapiens
54 TNFSF8  
Affinity Capture-MS Homo sapiens
55 RFC2 5982
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
56 CUL3 8452
Affinity Capture-MS Homo sapiens
57 Rrbp1  
Affinity Capture-MS Mus musculus
58 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
59 KLRD1  
Affinity Capture-MS Homo sapiens
60 FBXO6 26270
Affinity Capture-MS Homo sapiens
61 RFC1 5981
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
62 C12orf74  
Affinity Capture-MS Homo sapiens
63 MYC  
Affinity Capture-MS Homo sapiens
64 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
65 RPP40 10799
Co-fractionation Homo sapiens
66 PVR 5817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 DDRGK1 65992
Affinity Capture-MS Homo sapiens
68 Rpl35 66489
Affinity Capture-MS Mus musculus
69 ISLR 3671
Affinity Capture-MS Homo sapiens
70 NFATC2  
Affinity Capture-MS Homo sapiens
71 C9orf72  
Affinity Capture-MS Homo sapiens
72 CCDC8  
Affinity Capture-MS Homo sapiens
73 HIF1A 3091
Affinity Capture-MS Homo sapiens
74 SURF6  
Affinity Capture-MS Homo sapiens
75 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
76 SIRT6  
Affinity Capture-MS Homo sapiens
77 RAD17  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 HDGF 3068
Affinity Capture-MS Homo sapiens
79 FBXL6  
Affinity Capture-MS Homo sapiens
80 TP53 7157
Affinity Capture-MS Homo sapiens
81 LDLRAD1  
Affinity Capture-MS Homo sapiens
82 DSCC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 CHTF18 63922
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
84 RPA1 6117
Affinity Capture-MS Homo sapiens
85 INCA1  
Two-hybrid Homo sapiens
86 MAGEA9  
Affinity Capture-MS Homo sapiens
87 PCNA 5111
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
88 UFL1 23376
Affinity Capture-MS Homo sapiens
89 KHDRBS2  
Two-hybrid Homo sapiens
90 CEBPA  
Protein-peptide Homo sapiens
91 NCL 4691
Affinity Capture-MS Homo sapiens
92 PCBP1 5093
Affinity Capture-MS Homo sapiens
93 C16orf72 29035
Affinity Capture-MS Homo sapiens
94 IBTK 25998
Affinity Capture-MS Homo sapiens
95 CDK9 1025
Affinity Capture-MS Homo sapiens
96 SSSCA1 10534
Affinity Capture-MS Homo sapiens
97 CIT 11113
Affinity Capture-MS Homo sapiens
98 ORC1  
Affinity Capture-MS Homo sapiens
99 SERBP1 26135
Affinity Capture-MS Homo sapiens
100 SNW1 22938
Affinity Capture-MS Homo sapiens
101 KRAS 3845
Negative Genetic Homo sapiens
102 HNRNPU 3192
Affinity Capture-MS Homo sapiens
103 ARFGAP1 55738
Two-hybrid Homo sapiens
104 RC3H1 149041
Affinity Capture-MS Homo sapiens
105 SSRP1 6749
Affinity Capture-MS Homo sapiens
106 PURA 5813
Co-fractionation Homo sapiens
107 USP20  
Affinity Capture-MS Homo sapiens
108 EP300 2033
Affinity Capture-MS Homo sapiens
109 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RFC3 is involved
PathwayEvidenceSource
Activation of ATR in response to replication stress TAS Reactome
Base Excision Repair TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Chromosome Maintenance TAS Reactome
Defective homologous recombination repair (HRR) due to BRCA2 loss of function TAS Reactome
Disease TAS Reactome
Diseases of DNA Double-Strand Break Repair TAS Reactome
Diseases of DNA repair TAS Reactome
DNA Damage Bypass TAS Reactome
DNA Double-Strand Break Repair TAS Reactome
DNA Repair TAS Reactome
DNA Replication TAS Reactome
DNA strand elongation TAS Reactome
Dual Incision in GG-NER TAS Reactome
Dual incision in TC-NER TAS Reactome
Extension of Telomeres TAS Reactome
G2/M Checkpoints TAS Reactome
G2/M DNA damage checkpoint TAS Reactome
Gap-filling DNA repair synthesis and ligation in GG-NER TAS Reactome
Gap-filling DNA repair synthesis and ligation in TC-NER TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Global Genome Nucleotide Excision Repair (GG-NER) TAS Reactome
HDR through Homologous Recombination (HRR) TAS Reactome
HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) TAS Reactome
HDR through Single Strand Annealing (SSA) TAS Reactome
Homologous DNA Pairing and Strand Exchange TAS Reactome
Homology Directed Repair TAS Reactome
Impaired BRCA2 binding to RAD51 TAS Reactome
Lagging Strand Synthesis TAS Reactome
Leading Strand Synthesis TAS Reactome
Nucleotide Excision Repair TAS Reactome
PCNA-Dependent Long Patch Base Excision Repair TAS Reactome
Polymerase switching TAS Reactome
Polymerase switching on the C-strand of the telomere TAS Reactome
Presynaptic phase of homologous DNA pairing and strand exchange TAS Reactome
Processing of DNA double-strand break ends TAS Reactome
Recognition of DNA damage by PCNA-containing replication complex TAS Reactome
Regulation of TP53 Activity TAS Reactome
Regulation of TP53 Activity through Phosphorylation TAS Reactome
Resolution of Abasic Sites (AP sites) TAS Reactome
Resolution of AP sites via the multiple-nucleotide patch replacement pathway TAS Reactome
RNA Polymerase II Transcription TAS Reactome
S Phase TAS Reactome
Synthesis of DNA TAS Reactome
Telomere C-strand (Lagging Strand) Synthesis TAS Reactome
Telomere Maintenance TAS Reactome
Termination of translesion DNA synthesis TAS Reactome
Transcription-Coupled Nucleotide Excision Repair (TC-NER) TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
Translesion Synthesis by POLH TAS Reactome
Translesion synthesis by POLI TAS Reactome
Translesion synthesis by POLK TAS Reactome
Translesion synthesis by REV1 TAS Reactome
Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template TAS Reactome





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