Gene description for Pcna
Gene name proliferating cell nuclear antigen
Gene symbol Pcna
Other names/aliases -
Species Mus musculus
 Database cross references - Pcna
ExoCarta ExoCarta_18538
Vesiclepedia VP_18538
Entrez Gene 18538
UniProt P17918  
 Pcna identified in sEVs derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Fibroblasts 23260141    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pcna
Molecular Function
    purine-specific mismatch base pair DNA N-glycosylase activity GO:0000701 IEA
    purine-specific mismatch base pair DNA N-glycosylase activity GO:0000701 ISO
    chromatin binding GO:0003682 ISO
    chromatin binding GO:0003682 ISS
    damaged DNA binding GO:0003684 ISO
    damaged DNA binding GO:0003684 ISS
    protein binding GO:0005515 IPI
    transcription factor binding GO:0008134 IPI
    enzyme binding GO:0019899 IPI
    enzyme binding GO:0019899 ISO
    nuclear estrogen receptor binding GO:0030331 IEA
    nuclear estrogen receptor binding GO:0030331 ISO
    DNA polymerase processivity factor activity GO:0030337 IBA
    DNA polymerase processivity factor activity GO:0030337 IEA
    receptor tyrosine kinase binding GO:0030971 IEA
    receptor tyrosine kinase binding GO:0030971 ISO
    dinucleotide insertion or deletion binding GO:0032139 IEA
    dinucleotide insertion or deletion binding GO:0032139 ISO
    MutLalpha complex binding GO:0032405 IEA
    MutLalpha complex binding GO:0032405 ISO
    histone acetyltransferase binding GO:0035035 IEA
    histone acetyltransferase binding GO:0035035 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 ISO
    DNA polymerase binding GO:0070182 IEA
    DNA polymerase binding GO:0070182 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    leading strand elongation GO:0006272 IBA
    base-excision repair, gap-filling GO:0006287 IDA
    mismatch repair GO:0006298 IBA
    mismatch repair GO:0006298 IEA
    mismatch repair GO:0006298 ISO
    heart development GO:0007507 IEA
    translesion synthesis GO:0019985 IBA
    translesion synthesis GO:0019985 ISO
    translesion synthesis GO:0019985 ISS
    epithelial cell differentiation GO:0030855 IEA
    replication fork processing GO:0031297 IGI
    positive regulation of deoxyribonuclease activity GO:0032077 ISO
    positive regulation of deoxyribonuclease activity GO:0032077 ISS
    response to estradiol GO:0032355 IEA
    response to lipid GO:0033993 ISO
    cellular response to UV GO:0034644 ISO
    cellular response to UV GO:0034644 ISS
    estrous cycle GO:0044849 IEA
    positive regulation of DNA repair GO:0045739 ISO
    positive regulation of DNA repair GO:0045739 ISS
    positive regulation of DNA replication GO:0045740 ISO
    positive regulation of DNA replication GO:0045740 ISS
    response to cadmium ion GO:0046686 IEA
    cellular response to hydrogen peroxide GO:0070301 IEA
    cellular response to xenobiotic stimulus GO:0071466 IDA
    response to dexamethasone GO:0071548 IEA
    liver regeneration GO:0097421 IEA
    positive regulation of DNA-directed DNA polymerase activity GO:1900264 ISO
    response to L-glutamate GO:1902065 IEA
    mitotic telomere maintenance via semi-conservative replication GO:1902990 IGI
Subcellular Localization
    cyclin-dependent protein kinase holoenzyme complex GO:0000307 IPI
    chromatin GO:0000785 ISO
    chromatin GO:0000785 ISS
    male germ cell nucleus GO:0001673 IDA
    intracellular anatomical structure GO:0005622 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nuclear lamina GO:0005652 IDA
    nucleoplasm GO:0005654 ISO
    replication fork GO:0005657 IDA
    replication fork GO:0005657 ISO
    centrosome GO:0005813 ISO
    nuclear body GO:0016604 IEA
    nuclear body GO:0016604 ISO
    nuclear replication fork GO:0043596 IEA
    nuclear replication fork GO:0043596 ISO
    PCNA complex GO:0043626 IBA
    PCNA complex GO:0043626 ISO
    PCNA complex GO:0043626 ISS
    PCNA-p21 complex GO:0070557 ISO
    PCNA-p21 complex GO:0070557 ISS
 Experiment description of studies that identified Pcna in sEVs
1
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pcna
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Polh  
Two-hybrid Mus musculus
2 Polk  
Affinity Capture-Western Mus musculus
3 APEX2  
Affinity Capture-MS Homo sapiens
4 PKN3 29941
Affinity Capture-MS Homo sapiens
5 KIAA0101  
Affinity Capture-MS Homo sapiens
6 Pidd1  
Affinity Capture-Western Mus musculus
7 RNF219  
Affinity Capture-MS Homo sapiens
8 MSH2 4436
Affinity Capture-MS Homo sapiens
9 PCNA 5111
Affinity Capture-MS Homo sapiens
10 Kcnma1  
Affinity Capture-MS Mus musculus
11 Cdk2  
Affinity Capture-Western Mus musculus
12 Pik3cb  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
13 CENPJ 55835
Affinity Capture-MS Homo sapiens
14 RBBP5 5929
Affinity Capture-MS Homo sapiens
15 Atxn1  
Affinity Capture-MS Mus musculus
16 POLD1 5424
Affinity Capture-MS Homo sapiens
17 Cdk4  
Affinity Capture-Western Mus musculus
18 Tfe3  
Affinity Capture-MS Mus musculus
19 MRPL54  
Affinity Capture-MS Homo sapiens
20 Chaf1a  
Affinity Capture-MS Mus musculus
21 EPPK1 83481
Affinity Capture-MS Homo sapiens
22 Dnmt1 13433
Affinity Capture-Western Mus musculus
23 Igf1r 16001
Affinity Capture-Western Mus musculus
24 MRPL53  
Affinity Capture-MS Homo sapiens
25 Ppp1ca 19045
Affinity Capture-Western Mus musculus
26 RFC1 5981
Affinity Capture-MS Homo sapiens
27 Mettl21e  
Affinity Capture-MS Mus musculus
28 CDKN1A  
Affinity Capture-MS Homo sapiens
29 Rev1  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Co-purification Mus musculus
30 XPO5 57510
Affinity Capture-MS Homo sapiens
31 Polb  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
32 CCNB1 891
Two-hybrid Homo sapiens
33 UBFD1 56061
Affinity Capture-MS Homo sapiens
34 SIVA1  
Affinity Capture-MS Homo sapiens
35 RFC2 5982
Affinity Capture-MS Homo sapiens
36 Agap2  
Affinity Capture-MS Mus musculus
37 TEFM  
Affinity Capture-MS Homo sapiens
38 Apex1  
Affinity Capture-Western Mus musculus
39 POLD3  
Affinity Capture-MS Homo sapiens
40 APOB 338
Affinity Capture-MS Homo sapiens
41 TCEB2 6923
Affinity Capture-MS Homo sapiens
42 Nbn  
Co-localization Mus musculus
43 ANKHD1 54882
Affinity Capture-MS Homo sapiens
44 Eed  
Affinity Capture-MS Mus musculus
45 Cdkn1a  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
46 DNMT1 1786
Affinity Capture-MS Homo sapiens
47 USP32 84669
Affinity Capture-MS Homo sapiens
48 POLD2 5425
Affinity Capture-MS Homo sapiens
49 Ppp1cc 19047
Affinity Capture-Western Mus musculus
50 Gadd45a  
Co-localization Mus musculus
Affinity Capture-Western Mus musculus
51 Fancd2  
Affinity Capture-MS Mus musculus
52 Tcf3  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Pcna is involved
PathwayEvidenceSource
Base Excision Repair IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Chromosome Maintenance IEA Reactome
DNA Damage Bypass IEA Reactome
DNA Double-Strand Break Repair IEA Reactome
DNA Repair IEA Reactome
DNA Replication IEA Reactome
DNA strand elongation IEA Reactome
Dual Incision in GG-NER IEA Reactome
Dual incision in TC-NER IEA Reactome
E3 ubiquitin ligases ubiquitinate target proteins IEA Reactome
Extension of Telomeres IEA Reactome
Gap-filling DNA repair synthesis and ligation in GG-NER IEA Reactome
Gap-filling DNA repair synthesis and ligation in TC-NER IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
Global Genome Nucleotide Excision Repair (GG-NER) IEA Reactome
HDR through Homologous Recombination (HRR) IEA Reactome
HDR through Homologous Recombination (HRR) or Single Strand Annealing (SSA) IEA Reactome
Homology Directed Repair IEA Reactome
Lagging Strand Synthesis IEA Reactome
Leading Strand Synthesis IEA Reactome
Metabolism of proteins IEA Reactome
Mismatch Repair IEA Reactome
Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) IEA Reactome
Nucleotide Excision Repair IEA Reactome
PCNA-Dependent Long Patch Base Excision Repair IEA Reactome
Polymerase switching IEA Reactome
Polymerase switching on the C-strand of the telomere IEA Reactome
Post-translational protein modification IEA Reactome
Processive synthesis on the C-strand of the telomere IEA Reactome
Processive synthesis on the lagging strand IEA Reactome
Protein ubiquitination IEA Reactome
Recognition of DNA damage by PCNA-containing replication complex IEA Reactome
Removal of the Flap Intermediate IEA Reactome
Removal of the Flap Intermediate from the C-strand IEA Reactome
Resolution of Abasic Sites (AP sites) IEA Reactome
Resolution of AP sites via the multiple-nucleotide patch replacement pathway IEA Reactome
RNA Polymerase II Transcription IEA Reactome
S Phase IEA Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation IEA Reactome
SUMOylation of DNA replication proteins IEA Reactome
Synthesis of DNA IEA Reactome
Telomere C-strand (Lagging Strand) Synthesis IEA Reactome
Telomere Maintenance IEA Reactome
Termination of translesion DNA synthesis IEA Reactome
TP53 Regulates Transcription of Cell Cycle Genes IEA Reactome
TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest IEA Reactome
Transcription-Coupled Nucleotide Excision Repair (TC-NER) IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome
Translesion Synthesis by POLH IEA Reactome
Translesion synthesis by POLI IEA Reactome
Translesion synthesis by POLK IEA Reactome
Translesion synthesis by REV1 IEA Reactome
Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template IEA Reactome





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