Gene description for CENPJ
Gene name centromere protein J
Gene symbol CENPJ
Other names/aliases BM032
CENP-J
CPAP
LAP
LIP1
MCPH6
SASS4
SCKL4
Sas-4
Species Homo sapiens
 Database cross references - CENPJ
ExoCarta ExoCarta_55835
Vesiclepedia VP_55835
Entrez Gene 55835
HGNC 17272
MIM 609279
UniProt Q9HC77  
 CENPJ identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CENPJ
Molecular Function
    transcription coactivator activity GO:0003713 IDA
    protein binding GO:0005515 IPI
    tubulin binding GO:0015631 IBA
    tubulin binding GO:0015631 IDA
    protein kinase binding GO:0019901 IPI
    protein domain specific binding GO:0019904 IPI
    identical protein binding GO:0042802 IPI
    gamma-tubulin binding GO:0043015 IDA
Biological Process
    microtubule nucleation GO:0007020 TAS
    centriole replication GO:0007099 IMP
    smoothened signaling pathway GO:0007224 IEA
    astral microtubule nucleation GO:0030954 IDA
    motile cilium assembly GO:0044458 IEA
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of receptor signaling pathway via JAK-STAT GO:0046427 IDA
    regulation of centriole replication GO:0046599 IMP
    positive regulation of centriole replication GO:0046601 IDA
    microtubule polymerization GO:0046785 IMP
    cell division GO:0051301 NAS
    regulation of mitotic spindle organization GO:0060236 NAS
    cilium assembly GO:0060271 IBA
    centriole elongation GO:0061511 IBA
    centriole elongation GO:0061511 IDA
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 IDA
    positive regulation of non-motile cilium assembly GO:1902857 IEA
    positive regulation of centriole elongation GO:1903724 IMP
    positive regulation of establishment of protein localization GO:1904951 IMP
    non-motile cilium assembly GO:1905515 IEA
    positive regulation of spindle assembly GO:1905832 NAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    centriole GO:0005814 IBA
    centriole GO:0005814 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IEA
    gamma-tubulin small complex GO:0008275 NAS
    ciliary basal body GO:0036064 IEA
    procentriole replication complex GO:0120099 IPI
 Experiment description of studies that identified CENPJ in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CENPJ
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 Stat5a  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
3 RELA 5970
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 CREBBP  
Reconstituted Complex Homo sapiens
Phenotypic Enhancement Homo sapiens
5 SNX27 81609
Affinity Capture-MS Homo sapiens
6 DPPA3  
Affinity Capture-MS Homo sapiens
7 CRTC3  
Affinity Capture-MS Homo sapiens
8 KLC2 64837
Affinity Capture-MS Homo sapiens
9 CEP131 22994
Affinity Capture-MS Homo sapiens
10 Bap1  
Affinity Capture-MS Mus musculus
11 SLMAP 7871
Proximity Label-MS Homo sapiens
12 GFI1B  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
13 TP53 7157
Affinity Capture-MS Homo sapiens
14 Vim 22352
Affinity Capture-MS Mus musculus
15 TUBB3 10381
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TUBG1 7283
Affinity Capture-Western Homo sapiens
17 NINL  
Proximity Label-MS Homo sapiens
18 CCP110  
Affinity Capture-MS Homo sapiens
19 KRT8 3856
Proximity Label-MS Homo sapiens
20 GEMIN5 25929
Affinity Capture-MS Homo sapiens
21 PCNT  
Proximity Label-MS Homo sapiens
22 NAB1 4664
Affinity Capture-MS Homo sapiens
23 TUBB8 347688
Affinity Capture-MS Homo sapiens
24 PHLDB3  
Affinity Capture-MS Homo sapiens
25 YWHAZ 7534
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
26 NABP2  
Affinity Capture-MS Homo sapiens
27 TUBB4B 10383
Affinity Capture-MS Homo sapiens
28 SH3RF2  
Affinity Capture-MS Homo sapiens
29 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ATN1  
Two-hybrid Homo sapiens
31 DUSP16  
Affinity Capture-MS Homo sapiens
32 SASS6 163786
Proximity Label-MS Homo sapiens
33 LATS1  
Proximity Label-MS Homo sapiens
34 NXF1 10482
Affinity Capture-RNA Homo sapiens
35 ANAPC2 29882
Proximity Label-MS Homo sapiens
36 PPHLN1  
Affinity Capture-MS Homo sapiens
37 AMOT 154796
Proximity Label-MS Homo sapiens
38 MAPRE3  
Proximity Label-MS Homo sapiens
39 PRR3  
Affinity Capture-MS Homo sapiens
40 IBSP  
Affinity Capture-MS Homo sapiens
41 MOV10 4343
Affinity Capture-RNA Homo sapiens
42 Stat5b  
Affinity Capture-Western Mus musculus
43 YWHAE 7531
Affinity Capture-MS Homo sapiens
44 Kif4  
Affinity Capture-MS Mus musculus
45 CLASP2 23122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 CEP135  
Proximity Label-MS Homo sapiens
47 CLTA 1211
Affinity Capture-MS Homo sapiens
48 DYNLRB1 83658
Affinity Capture-MS Homo sapiens
49 EPB41 2035
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
50 BCCIP 56647
Two-hybrid Homo sapiens
51 DCTN1 1639
Proximity Label-MS Homo sapiens
52 NDC80 10403
Proximity Label-MS Homo sapiens
53 TUBA1B 10376
Affinity Capture-MS Homo sapiens
54 PLEKHO2 80301
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 CIC 23152
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
56 MRPL12 6182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 Pcna 18538
Affinity Capture-MS Mus musculus
58 STIL  
Proximity Label-MS Homo sapiens
59 PCM1 5108
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 YWHAG 7532
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
61 LYST 1130
Two-hybrid Homo sapiens
62 NR3C1 2908
Proximity Label-MS Homo sapiens
63 LATS2 26524
Proximity Label-MS Homo sapiens
64 NIN 51199
Proximity Label-MS Homo sapiens
65 CLASP1 23332
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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