Gene description for Vim
Gene name vimentin
Gene symbol Vim
Other names/aliases -
Species Mus musculus
 Database cross references - Vim
ExoCarta ExoCarta_22352
Vesiclepedia VP_22352
Entrez Gene 22352
UniProt P20152  
 Vim identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mov neuroglial cells 15210972    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Vim
Molecular Function
    RNA binding GO:0003723 IPI
    double-stranded RNA binding GO:0003725 ISO
    structural constituent of cytoskeleton GO:0005200 IBA
    structural constituent of cytoskeleton GO:0005200 IMP
    structural constituent of cytoskeleton GO:0005200 ISO
    structural constituent of eye lens GO:0005212 IDA
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein phosphatase 2A binding GO:0051721 ISO
    molecular adaptor activity GO:0060090 ISO
    scaffold protein binding GO:0097110 IEA
    scaffold protein binding GO:0097110 ISO
    keratin filament binding GO:1990254 IEA
    keratin filament binding GO:1990254 ISO
    protein tyrosine kinase binding GO:1990782 ISO
Biological Process
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of epithelial cell migration GO:0010634 ISS
    negative regulation of neuron projection development GO:0010977 IGI
    astrocyte development GO:0014002 IGI
    neuron projection development GO:0031175 IGI
    positive regulation of collagen biosynthetic process GO:0032967 IEA
    positive regulation of collagen biosynthetic process GO:0032967 ISO
    regulation of mRNA stability GO:0043488 IEA
    regulation of mRNA stability GO:0043488 ISO
    intermediate filament-based process GO:0045103 IMP
    intermediate filament organization GO:0045109 IBA
    intermediate filament organization GO:0045109 IGI
    intermediate filament organization GO:0045109 IMP
    regulation of axonogenesis GO:0050770 ISO
    Bergmann glial cell differentiation GO:0060020 IMP
    positive regulation of glial cell proliferation GO:0060252 ISO
    lens fiber cell development GO:0070307 IDA
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to lipopolysaccharide GO:0071222 ISS
    cellular response to muramyl dipeptide GO:0071225 ISO
    cellular response to muramyl dipeptide GO:0071225 ISS
    cellular response to type II interferon GO:0071346 IDA
    regulation of Schwann cell migration GO:1900147 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    peroxisome GO:0005777 IEA
    peroxisome GO:0005777 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytoskeleton GO:0005856 ISO
    intermediate filament GO:0005882 IBA
    intermediate filament GO:0005882 IDA
    intermediate filament GO:0005882 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    nuclear matrix GO:0016363 IEA
    axon GO:0030424 IBA
    axon GO:0030424 ISO
    cell leading edge GO:0031252 IDA
    cell projection GO:0042995 IDA
    cell projection GO:0042995 ISO
    neuron projection GO:0043005 IDA
    cell body GO:0044297 ISO
    type III intermediate filament GO:0045098 TAS
    intermediate filament cytoskeleton GO:0045111 ISO
    phagocytic vesicle GO:0045335 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Vim in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Vim
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fxr1 14359
Affinity Capture-MS Mus musculus
2 CENPJ 55835
Affinity Capture-MS Homo sapiens
3 Pde4dip  
Affinity Capture-MS Mus musculus
4 ZBTB11  
Affinity Capture-MS Homo sapiens
5 Rab8b 235442
Co-fractionation Mus musculus
6 Pla2g4a  
Far Western Mus musculus
7 Hspb2 69253
Affinity Capture-MS Mus musculus
8 Vim 22352
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
9 Kcnma1  
Affinity Capture-MS Mus musculus
10 Pparg  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
11 Tmem173  
Proximity Label-MS Mus musculus
12 Atxn1  
Proximity Label-MS Mus musculus
13 Kcna3  
Affinity Capture-MS Mus musculus
14 Kctd13  
Affinity Capture-MS Mus musculus
15 Tfe3  
Affinity Capture-MS Mus musculus
16 Cbx1  
Affinity Capture-MS Mus musculus
17 Urod  
Co-fractionation Mus musculus
18 Nphp4  
Affinity Capture-MS Mus musculus
19 PHB2 11331
Affinity Capture-MS Homo sapiens
20 Rock2  
Biochemical Activity Mus musculus
21 Pdlim1 54132
Co-fractionation Mus musculus
22 RAB7A 7879
Affinity Capture-MS Homo sapiens
23 SYNC  
Affinity Capture-MS Homo sapiens
24 Cbx3 12417
Affinity Capture-MS Mus musculus
25 HACE1 57531
Affinity Capture-MS Homo sapiens
26 Invs  
Affinity Capture-MS Mus musculus
27 Npm1 18148
Affinity Capture-MS Mus musculus
28 Ppl  
Affinity Capture-Western Mus musculus
29 Txn1 22166
Co-fractionation Mus musculus
30 Anxa2 12306
Co-fractionation Mus musculus
31 Eed  
Affinity Capture-MS Mus musculus
32 Gfap  
Co-fractionation Mus musculus
33 Nphp1  
Affinity Capture-MS Mus musculus
34 Fancd2  
Affinity Capture-MS Mus musculus
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