Gene description for Txn1
Gene name thioredoxin 1
Gene symbol Txn1
Other names/aliases ADF
AW550880
Trx1
Txn
Species Mus musculus
 Database cross references - Txn1
ExoCarta ExoCarta_22166
Vesiclepedia VP_22166
Entrez Gene 22166
UniProt P10639  
 Txn1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Txn1
Molecular Function
    protein binding GO:0005515 IPI
    protein-disulfide reductase activity GO:0015035 IDA
    protein-disulfide reductase activity GO:0015035 ISO
    enzyme binding GO:0019899 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    protein-disulfide reductase (NAD(P)H) activity GO:0047134 IEA
    protein-disulfide reductase (NAD(P)H) activity GO:0047134 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IGI
    response to radiation GO:0009314 ISO
    response to radiation GO:0009314 ISS
    positive regulation of peptidyl-serine phosphorylation GO:0033138 ISO
    positive regulation of DNA binding GO:0043388 ISO
    positive regulation of DNA binding GO:0043388 ISS
    cell redox homeostasis GO:0045454 IDA
    cell redox homeostasis GO:0045454 ISO
    negative regulation of protein export from nucleus GO:0046826 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IEA
    cellular detoxification of hydrogen peroxide GO:0061692 IEA
    cellular detoxification of hydrogen peroxide GO:0061692 ISO
    response to nitric oxide GO:0071731 IEA
    response to nitric oxide GO:0071731 ISO
    positive regulation of peptidyl-cysteine S-nitrosylation GO:2000170 IEA
    positive regulation of peptidyl-cysteine S-nitrosylation GO:2000170 ISO
Subcellular Localization
    extracellular region GO:0005576 IEA
    extracellular region GO:0005576 ISO
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    axon GO:0030424 ISO
    dendrite GO:0030425 ISO
    neuronal cell body GO:0043025 ISO
 Experiment description of studies that identified Txn1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Txn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CFAP20 29105
Two-hybrid Homo sapiens
2 NXF1 10482
Two-hybrid Homo sapiens
3 Mettl21e  
Affinity Capture-MS Mus musculus
4 Uchl5 56207
Co-fractionation Mus musculus
5 Srp9  
Co-fractionation Mus musculus
6 Atxn1  
Proximity Label-MS Mus musculus
7 Eed  
Affinity Capture-MS Mus musculus
8 MAPT  
Reconstituted Complex Homo sapiens
9 Ube2i  
Two-hybrid Mus musculus
10 Rpl32 19951
Co-fractionation Mus musculus
11 Map3k5  
Affinity Capture-Western Mus musculus
12 HNRNPA0 10949
Two-hybrid Homo sapiens
13 Unk  
Affinity Capture-RNA Mus musculus
14 Vim 22352
Co-fractionation Mus musculus
15 Fancd2  
Affinity Capture-MS Mus musculus
16 Kctd13  
Affinity Capture-MS Mus musculus
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