Gene description for Uchl5
Gene name ubiquitin carboxyl-terminal esterase L5
Gene symbol Uchl5
Other names/aliases 5830413B11Rik
Uch37
Species Mus musculus
 Database cross references - Uchl5
ExoCarta ExoCarta_56207
Vesiclepedia VP_56207
Entrez Gene 56207
UniProt Q9WUP7  
 Uchl5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Uchl5
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 ISO
    cysteine-type deubiquitinase activity GO:0004843 ISS
    cysteine-type deubiquitinase activity GO:0004843 TAS
    endopeptidase inhibitor activity GO:0004866 IEA
    endopeptidase inhibitor activity GO:0004866 ISO
    proteasome binding GO:0070628 IEA
    proteasome binding GO:0070628 ISO
Biological Process
    telomere maintenance GO:0000723 IMP
    regulation of DNA replication GO:0006275 ISO
    DNA repair GO:0006281 IEA
    regulation of DNA repair GO:0006282 IDA
    DNA recombination GO:0006310 IEA
    chromatin remodeling GO:0006338 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    protein deubiquitination GO:0016579 ISO
    protein deubiquitination GO:0016579 ISS
    lateral ventricle development GO:0021670 IMP
    midbrain development GO:0030901 IMP
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 IEA
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 ISO
    regulation of chromosome organization GO:0033044 IBA
    regulation of chromosome organization GO:0033044 ISO
    positive regulation of DNA repair GO:0045739 IDA
    positive regulation of smoothened signaling pathway GO:0045880 IEA
    positive regulation of smoothened signaling pathway GO:0045880 ISO
    positive regulation of DNA-templated transcription GO:0045893 ISO
    regulation of embryonic development GO:0045995 IMP
    forebrain morphogenesis GO:0048853 IMP
    regulation of cell cycle GO:0051726 ISO
    regulation of DNA strand elongation GO:0060382 ISO
    regulation of proteasomal protein catabolic process GO:0061136 ISO
    positive regulation of telomere maintenance in response to DNA damage GO:1904507 IMP
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    Ino80 complex GO:0031011 ISO
    Ino80 complex GO:0031011 ISS
    cytosolic proteasome complex GO:0031597 IEA
    cytosolic proteasome complex GO:0031597 ISO
 Experiment description of studies that identified Uchl5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Uchl5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Psmb7 19177
Co-fractionation Mus musculus
2 Psmc3 19182
Co-fractionation Mus musculus
3 Ubc  
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
4 ADRM1 11047
Co-crystal Structure Homo sapiens
5 Pnp 18950
Co-fractionation Mus musculus
6 Ruvbl2 20174
Co-fractionation Mus musculus
7 Olfm1  
Affinity Capture-MS Mus musculus
8 Ubqln2  
Co-fractionation Mus musculus
9 Psmc6 67089
Co-fractionation Mus musculus
10 Txnl1 53382
Co-fractionation Mus musculus
11 Psma2 19166
Co-fractionation Mus musculus
12 Psmb6 19175
Co-fractionation Mus musculus
13 Psmb4 19172
Co-fractionation Mus musculus
14 Psma5 26442
Co-fractionation Mus musculus
15 Psmb5 19173
Co-fractionation Mus musculus
16 Psmd12 66997
Co-fractionation Mus musculus
17 Psmd13 23997
Co-fractionation Mus musculus
18 Psma1 26440
Co-fractionation Mus musculus
19 Ubqln4  
Co-fractionation Mus musculus
20 Cyb5b 66427
Co-fractionation Mus musculus
21 Capza1 12340
Co-fractionation Mus musculus
22 Prdx1 18477
Co-fractionation Mus musculus
23 Ubqln1  
Co-fractionation Mus musculus
24 UBC 7316
Co-crystal Structure Homo sapiens
25 Eef1g 67160
Co-fractionation Mus musculus
26 Rab7 19349
Co-fractionation Mus musculus
27 Ufd1l  
Co-fractionation Mus musculus
28 MTNR1A  
Reconstituted Complex Homo sapiens
29 Fth1 14319
Co-fractionation Mus musculus
30 Psmd8 57296
Co-fractionation Mus musculus
31 Pof1b  
Co-fractionation Mus musculus
32 Ino80d  
Co-fractionation Mus musculus
33 Serpinb5  
Co-fractionation Mus musculus
34 Rad23a  
Co-fractionation Mus musculus
35 Echs1  
Co-fractionation Mus musculus
36 Psmc1 19179
Co-fractionation Mus musculus
37 Psmd1 70247
Co-fractionation Mus musculus
38 Usp14 59025
Co-fractionation Mus musculus
39 Txn1 22166
Co-fractionation Mus musculus
40 Prdx3 11757
Co-fractionation Mus musculus
41 Psmd7 17463
Co-fractionation Mus musculus
42 Anxa4 11746
Co-fractionation Mus musculus
43 Fbxo7  
Co-fractionation Mus musculus
44 Psmf1  
Co-fractionation Mus musculus
45 Ublcp1  
Co-fractionation Mus musculus
46 Zfand2b  
Co-fractionation Mus musculus
47 Rps21 66481
Co-fractionation Mus musculus
48 Tgm3  
Co-fractionation Mus musculus
49 Anxa1 16952
Co-fractionation Mus musculus
50 Actg1 11465
Co-fractionation Mus musculus
51 Nanog  
Affinity Capture-MS Mus musculus
52 Psmd3 22123
Co-fractionation Mus musculus
53 Psmb1 19170
Co-fractionation Mus musculus
54 Psme1 19186
Co-fractionation Mus musculus
55 Psmc4 23996
Co-fractionation Mus musculus
56 Prrc2a  
Co-fractionation Mus musculus
57 Adrm1  
Co-fractionation Mus musculus
58 Psma6 26443
Co-fractionation Mus musculus
59 Ctsd 13033
Co-fractionation Mus musculus
60 Hspa5 14828
Co-fractionation Mus musculus
61 Tpi1 21991
Co-fractionation Mus musculus
62 Psma4 26441
Co-fractionation Mus musculus
63 Ube3a  
Co-fractionation Mus musculus
64 Psmd6 66413
Co-fractionation Mus musculus
65 Ino80  
Co-fractionation Mus musculus
66 Ubfd1  
Co-fractionation Mus musculus
67 Cap1 12331
Co-fractionation Mus musculus
68 Fancd2  
Affinity Capture-MS Mus musculus
69 Psmb3 26446
Co-fractionation Mus musculus
70 Psmd11 69077
Co-fractionation Mus musculus
71 Krt80  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Uchl5 is involved
PathwayEvidenceSource
Deubiquitination IEA Reactome
Metabolism of proteins IEA Reactome
Post-translational protein modification IEA Reactome
UCH proteinases IEA Reactome





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