Gene description for Ctsd
Gene name cathepsin D
Gene symbol Ctsd
Other names/aliases CD
CatD
Species Mus musculus
 Database cross references - Ctsd
ExoCarta ExoCarta_13033
Vesiclepedia VP_13033
Entrez Gene 13033
UniProt P18242  
 Ctsd identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ctsd
Molecular Function
    endopeptidase activity GO:0004175 IDA
    endopeptidase activity GO:0004175 ISO
    aspartic-type endopeptidase activity GO:0004190 IBA
    aspartic-type endopeptidase activity GO:0004190 IDA
    protein binding GO:0005515 IPI
    peptidase activity GO:0008233 IDA
    peptidase activity GO:0008233 ISO
    hydrolase activity GO:0016787 IDA
    peptide binding GO:0042277 ISO
    aspartic-type peptidase activity GO:0070001 IMP
Biological Process
    autophagosome assembly GO:0000045 IMP
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IC
    proteolysis GO:0006508 ISO
    protein catabolic process GO:0030163 ISO
    insulin receptor recycling GO:0038020 IDA
    lipoprotein catabolic process GO:0042159 IEA
    lipoprotein catabolic process GO:0042159 ISO
    positive regulation of apoptotic process GO:0043065 IEA
    positive regulation of apoptotic process GO:0043065 ISO
    epithelial tube branching involved in lung morphogenesis GO:0060441 IMP
    regulation of establishment of protein localization GO:0070201 IEA
    regulation of establishment of protein localization GO:0070201 ISO
    execution phase of apoptosis GO:0097194 IEA
    insulin catabolic process GO:1901143 IDA
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    mitochondrion GO:0005739 HDA
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    lysosome GO:0005764 ISO
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    cytosol GO:0005829 IEA
    endosome membrane GO:0010008 IEA
    endosome membrane GO:0010008 ISO
    endosome lumen GO:0031904 IDA
    melanosome GO:0042470 IEA
    membrane raft GO:0045121 IEA
    membrane raft GO:0045121 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
    presynaptic endosome GO:0098830 ISO
    GABA-ergic synapse GO:0098982 ISO
 Experiment description of studies that identified Ctsd in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ctsd
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hbb 15127
Biochemical Activity Mus musculus
2 Hba-a1 15122
Biochemical Activity Mus musculus
3 Igf2r 16004
Affinity Capture-Western Mus musculus
4 Cst3  
Co-fractionation Mus musculus
5 Uchl5 56207
Co-fractionation Mus musculus
View the network image/svg+xml



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