Gene description for Ruvbl2
Gene name RuvB-like protein 2
Gene symbol Ruvbl2
Other names/aliases mp47
p47
Species Mus musculus
 Database cross references - Ruvbl2
ExoCarta ExoCarta_20174
Vesiclepedia VP_20174
Entrez Gene 20174
UniProt Q9WTM5  
 Ruvbl2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ruvbl2
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IEA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 IEA
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 ISO
    TFIID-class transcription factor complex binding GO:0001094 IPI
    DNA helicase activity GO:0003678 IBA
    DNA helicase activity GO:0003678 ISO
    DNA clamp loader activity GO:0003689 IEA
    transcription corepressor activity GO:0003714 IEA
    transcription corepressor activity GO:0003714 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    beta-catenin binding GO:0008013 IEA
    beta-catenin binding GO:0008013 ISO
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 ISO
    ATP hydrolysis activity GO:0016887 ISS
    TBP-class protein binding GO:0017025 IEA
    TBP-class protein binding GO:0017025 ISO
    chromatin DNA binding GO:0031490 ISO
    double-stranded DNA helicase activity GO:0036121 IEA
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IPI
    ADP binding GO:0043531 IDA
    ATPase binding GO:0051117 IPI
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    promoter-enhancer loop anchoring activity GO:0140585 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    box C/D snoRNP assembly GO:0000492 IBA
    telomere maintenance GO:0000723 IMP
    regulation of DNA replication GO:0006275 ISO
    DNA repair GO:0006281 IEA
    regulation of DNA repair GO:0006282 IDA
    DNA recombination GO:0006310 IEA
    chromatin remodeling GO:0006338 IBA
    chromatin remodeling GO:0006338 ISO
    regulation of DNA-templated transcription GO:0006355 NAS
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    DNA duplex unwinding GO:0032508 IEA
    regulation of chromosome organization GO:0033044 ISO
    cellular response to UV GO:0034644 IEA
    cellular response to UV GO:0034644 ISO
    regulation of apoptotic process GO:0042981 NAS
    positive regulation of DNA repair GO:0045739 IDA
    negative regulation of DNA-templated transcription GO:0045892 IEA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of DNA-templated transcription GO:0045893 NAS
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    regulation of embryonic development GO:0045995 IMP
    regulation of cell cycle GO:0051726 ISO
    regulation of DNA strand elongation GO:0060382 ISO
    establishment of protein localization to chromatin GO:0071169 IEA
    establishment of protein localization to chromatin GO:0071169 ISO
    cellular response to estradiol stimulus GO:0071392 IEA
    cellular response to estradiol stimulus GO:0071392 ISO
    negative regulation of canonical Wnt signaling pathway GO:0090090 IEA
    negative regulation of canonical Wnt signaling pathway GO:0090090 ISO
    chromatin looping GO:0140588 IEA
    positive regulation of telomere maintenance in response to DNA damage GO:1904507 IMP
    positive regulation of double-strand break repair via homologous recombination GO:1905168 ISO
    regulation of double-strand break repair GO:2000779 NAS
Subcellular Localization
    nucleosome GO:0000786 ISO
    nucleosome GO:0000786 NAS
    euchromatin GO:0000791 IEA
    euchromatin GO:0000791 ISO
    Swr1 complex GO:0000812 IBA
    Swr1 complex GO:0000812 ISO
    Swr1 complex GO:0000812 ISS
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    membrane GO:0016020 IEA
    nuclear matrix GO:0016363 IEA
    Ino80 complex GO:0031011 IBA
    Ino80 complex GO:0031011 ISO
    NuA4 histone acetyltransferase complex GO:0035267 IBA
    NuA4 histone acetyltransferase complex GO:0035267 ISO
    NuA4 histone acetyltransferase complex GO:0035267 ISS
    MLL1 complex GO:0071339 ISO
    MLL1 complex GO:0071339 ISS
    R2TP complex GO:0097255 IBA
    R2TP complex GO:0097255 ISO
    R2TP complex GO:0097255 ISS
    protein folding chaperone complex GO:0101031 IEA
    protein folding chaperone complex GO:0101031 ISO
    dynein axonemal particle GO:0120293 ISS
    RPAP3/R2TP/prefoldin-like complex GO:1990062 IEA
    RPAP3/R2TP/prefoldin-like complex GO:1990062 ISO
    ribonucleoprotein complex GO:1990904 IDA
 Experiment description of studies that identified Ruvbl2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ruvbl2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 INO80D  
Affinity Capture-MS Homo sapiens
2 YEATS4  
Affinity Capture-MS Homo sapiens
3 TTI1 9675
Affinity Capture-MS Homo sapiens
4 EPC2  
Affinity Capture-MS Homo sapiens
5 Upf1 19704
Co-fractionation Mus musculus
6 Rragc  
Co-fractionation Mus musculus
7 SUPT6H 6830
Affinity Capture-MS Homo sapiens
8 PFDN6 10471
Affinity Capture-MS Homo sapiens
9 NOP58 51602
Affinity Capture-MS Homo sapiens
10 Dkc1 245474
Co-fractionation Mus musculus
11 INO80C  
Affinity Capture-MS Homo sapiens
12 EPC1  
Affinity Capture-MS Homo sapiens
13 Ruvbl1 56505
Two-hybrid Mus musculus
14 ACTL6A 86
Affinity Capture-MS Homo sapiens
15 URI1  
Affinity Capture-MS Homo sapiens
16 TRRAP 8295
Affinity Capture-MS Homo sapiens
17 DPCD  
Affinity Capture-MS Homo sapiens
18 C12orf45  
Affinity Capture-MS Homo sapiens
19 INO80E  
Affinity Capture-MS Homo sapiens
20 Wdr1 22388
Affinity Capture-MS Mus musculus
21 WDR92 116143
Affinity Capture-MS Homo sapiens
22 Atxn1  
Affinity Capture-MS Mus musculus
23 INO80  
Affinity Capture-MS Homo sapiens
24 BRD8 10902
Affinity Capture-MS Homo sapiens
25 RPAP2  
Affinity Capture-MS Homo sapiens
26 NFRKB  
Affinity Capture-MS Homo sapiens
27 MORF4L2  
Affinity Capture-MS Homo sapiens
28 PDRG1  
Affinity Capture-MS Homo sapiens
29 Chaf1a  
Affinity Capture-MS Mus musculus
30 TFPT  
Affinity Capture-MS Homo sapiens
31 Ndrg1 17988
Co-fractionation Mus musculus
32 DMAP1 55929
Affinity Capture-MS Homo sapiens
33 POLR2A 5430
Affinity Capture-MS Homo sapiens
34 PRKDC 5591
Affinity Capture-MS Homo sapiens
35 Ighmbp2 20589
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
36 Mettl21e  
Affinity Capture-MS Mus musculus
37 YY1 7528
Affinity Capture-MS Homo sapiens
38 ACTR6  
Affinity Capture-MS Homo sapiens
39 Pfdn2  
Co-fractionation Mus musculus
40 Tardbp 230908
Co-fractionation Mus musculus
41 CDC42BPA 8476
Affinity Capture-MS Homo sapiens
42 MRGBP  
Affinity Capture-MS Homo sapiens
43 TTI2 80185
Affinity Capture-MS Homo sapiens
44 RUVBL1 8607
Affinity Capture-MS Homo sapiens
45 PFDN2 5202
Affinity Capture-MS Homo sapiens
46 ACTR8 93973
Affinity Capture-MS Homo sapiens
47 Ctr9  
Affinity Capture-MS Mus musculus
48 Ccar2  
Co-fractionation Mus musculus
49 Rpap3  
Co-fractionation Mus musculus
50 ZNHIT1  
Affinity Capture-MS Homo sapiens
51 Iqcb1  
Affinity Capture-MS Mus musculus
52 INO80B 83444
Affinity Capture-MS Homo sapiens
53 Actg1 11465
Co-fractionation Mus musculus
54 Nanog  
Affinity Capture-MS Mus musculus
55 MBTD1  
Affinity Capture-MS Homo sapiens
56 Tfcp2l1  
Affinity Capture-MS Mus musculus
57 Uchl5 56207
Co-fractionation Mus musculus
58 KAT5  
Affinity Capture-MS Homo sapiens
59 RPAP3 79657
Affinity Capture-MS Homo sapiens
60 Rnf2  
Affinity Capture-MS Mus musculus
61 ING3  
Affinity Capture-MS Homo sapiens
62 Fancd2  
Affinity Capture-MS Mus musculus
63 ACTR5 79913
Affinity Capture-MS Homo sapiens
64 Leo1  
Affinity Capture-MS Mus musculus
65 Eed  
Affinity Capture-MS Mus musculus
66 EP400  
Affinity Capture-MS Homo sapiens
67 Pfdn6  
Co-fractionation Mus musculus
68 ZNHIT2 741
Affinity Capture-MS Homo sapiens
69 PPP2R2A 5520
Affinity Capture-MS Homo sapiens
70 Appl1  
Co-fractionation Mus musculus
71 VPS72  
Affinity Capture-MS Homo sapiens
72 Atg16l1  
Affinity Capture-MS Mus musculus
73 Sall4  
Affinity Capture-MS Mus musculus
74 CHMP1B 57132
Affinity Capture-MS Homo sapiens
75 C2orf44  
Affinity Capture-MS Homo sapiens
76 PIH1D1  
Affinity Capture-MS Homo sapiens
77 Eftud2 20624
Co-fractionation Mus musculus
78 SRCAP  
Affinity Capture-MS Homo sapiens
79 Asna1 56495
Co-fractionation Mus musculus
80 UCHL5 51377
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Ruvbl2 is involved
No pathways found





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