Gene description for Ruvbl1
Gene name RuvB-like protein 1
Gene symbol Ruvbl1
Other names/aliases 2510009G06Rik
Pontin52
Tip49a
Species Mus musculus
 Database cross references - Ruvbl1
ExoCarta ExoCarta_56505
Vesiclepedia VP_56505
Entrez Gene 56505
UniProt P60122  
 Ruvbl1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ruvbl1
Molecular Function
    TFIID-class transcription factor complex binding GO:0001094 IPI
    DNA helicase activity GO:0003678 IBA
    DNA helicase activity GO:0003678 ISO
    DNA clamp loader activity GO:0003689 IEA
    transcription coactivator activity GO:0003713 IEA
    transcription coactivator activity GO:0003713 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 ISO
    ATP hydrolysis activity GO:0016887 ISS
    TBP-class protein binding GO:0017025 IEA
    TBP-class protein binding GO:0017025 ISO
    double-stranded DNA helicase activity GO:0036121 IEA
    3'-5' DNA helicase activity GO:0043138 ISO
    ADP binding GO:0043531 IDA
    ATPase binding GO:0051117 IPI
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    box C/D snoRNP assembly GO:0000492 IBA
    telomere maintenance GO:0000723 IMP
    regulation of DNA replication GO:0006275 ISO
    DNA repair GO:0006281 IEA
    regulation of DNA repair GO:0006282 IDA
    DNA recombination GO:0006310 IEA
    chromatin remodeling GO:0006338 IBA
    chromatin remodeling GO:0006338 ISO
    regulation of DNA-templated transcription GO:0006355 NAS
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    DNA duplex unwinding GO:0032508 IEA
    regulation of chromosome organization GO:0033044 ISO
    regulation of apoptotic process GO:0042981 NAS
    positive regulation of DNA repair GO:0045739 IDA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of DNA-templated transcription GO:0045893 NAS
    regulation of embryonic development GO:0045995 IMP
    cell division GO:0051301 IEA
    regulation of cell cycle GO:0051726 ISO
    regulation of DNA strand elongation GO:0060382 ISO
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 ISO
    chromatin looping GO:0140588 IEA
    positive regulation of telomere maintenance in response to DNA damage GO:1904507 IMP
    positive regulation of double-strand break repair via homologous recombination GO:1905168 ISO
    regulation of double-strand break repair GO:2000779 NAS
Subcellular Localization
    nucleosome GO:0000786 ISO
    nucleosome GO:0000786 NAS
    Swr1 complex GO:0000812 IBA
    Swr1 complex GO:0000812 ISO
    Swr1 complex GO:0000812 ISS
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    centrosome GO:0005813 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    membrane GO:0016020 IEA
    nuclear matrix GO:0016363 IEA
    nuclear matrix GO:0016363 ISO
    Ino80 complex GO:0031011 IBA
    Ino80 complex GO:0031011 ISO
    protein-containing complex GO:0032991 ISO
    NuA4 histone acetyltransferase complex GO:0035267 IBA
    NuA4 histone acetyltransferase complex GO:0035267 ISO
    NuA4 histone acetyltransferase complex GO:0035267 ISS
    MLL1 complex GO:0071339 ISO
    MLL1 complex GO:0071339 ISS
    R2TP complex GO:0097255 IBA
    R2TP complex GO:0097255 ISO
    R2TP complex GO:0097255 ISS
    protein folding chaperone complex GO:0101031 IEA
    protein folding chaperone complex GO:0101031 ISO
    dynein axonemal particle GO:0120293 ISS
    RPAP3/R2TP/prefoldin-like complex GO:1990062 IEA
    RPAP3/R2TP/prefoldin-like complex GO:1990062 ISO
    ribonucleoprotein complex GO:1990904 IDA
 Experiment description of studies that identified Ruvbl1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ruvbl1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MCRS1  
Affinity Capture-MS Homo sapiens
2 ZNHIT1  
Affinity Capture-MS Homo sapiens
3 MBTD1  
Affinity Capture-MS Homo sapiens
4 NOP58 51602
Affinity Capture-MS Homo sapiens
5 AATF  
Affinity Capture-MS Homo sapiens
6 TRRAP 8295
Affinity Capture-MS Homo sapiens
7 DPCD  
Affinity Capture-MS Homo sapiens
8 ZNHIT2 741
Affinity Capture-MS Homo sapiens
9 NFRKB  
Affinity Capture-MS Homo sapiens
10 Tfcp2l1  
Affinity Capture-MS Mus musculus
11 NDUFB5 4711
Affinity Capture-MS Homo sapiens
12 SURF4 6836
Affinity Capture-MS Homo sapiens
13 TMEM263 90488
Affinity Capture-MS Homo sapiens
14 AAR2 25980
Affinity Capture-MS Homo sapiens
15 PRKDC 5591
Affinity Capture-MS Homo sapiens
16 Ighmbp2 20589
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
17 URI1  
Affinity Capture-MS Homo sapiens
18 JAZF1  
Affinity Capture-MS Homo sapiens
19 Tmem173  
Proximity Label-MS Mus musculus
20 TBRG1  
Affinity Capture-MS Homo sapiens
21 SRCAP  
Affinity Capture-MS Homo sapiens
22 TBL3 10607
Affinity Capture-MS Homo sapiens
23 VPS72  
Affinity Capture-MS Homo sapiens
24 NUFIP1  
Affinity Capture-MS Homo sapiens
25 TELO2 9894
Affinity Capture-MS Homo sapiens
26 ING3  
Affinity Capture-MS Homo sapiens
27 Atg16l1  
Affinity Capture-MS Mus musculus
28 CHMP1B 57132
Affinity Capture-MS Homo sapiens
29 PAK1IP1  
Affinity Capture-MS Homo sapiens
30 C2orf44  
Affinity Capture-MS Homo sapiens
31 INO80E  
Affinity Capture-MS Homo sapiens
32 UCHL5 51377
Affinity Capture-MS Homo sapiens
33 GPN1  
Affinity Capture-MS Homo sapiens
34 PFDN6 10471
Affinity Capture-MS Homo sapiens
35 CHMP1A 5119
Affinity Capture-MS Homo sapiens
36 UXT 8409
Affinity Capture-MS Homo sapiens
37 PINX1  
Affinity Capture-MS Homo sapiens
38 POLR3K  
Affinity Capture-MS Homo sapiens
39 PSMC1 5700
Affinity Capture-MS Homo sapiens
40 CPS1 1373
Affinity Capture-MS Homo sapiens
41 PTCD3 55037
Affinity Capture-MS Homo sapiens
42 PFDN2 5202
Affinity Capture-MS Homo sapiens
43 CHMP4B 128866
Affinity Capture-MS Homo sapiens
44 ACTL6A 86
Affinity Capture-MS Homo sapiens
45 Nanog  
Affinity Capture-MS Mus musculus
46 Sqstm1  
Affinity Capture-MS Mus musculus
47 UBTF 7343
Affinity Capture-MS Homo sapiens
48 POLR2B 5431
Affinity Capture-MS Homo sapiens
49 YEATS4  
Affinity Capture-MS Homo sapiens
50 TTI1 9675
Affinity Capture-MS Homo sapiens
51 INO80D  
Affinity Capture-MS Homo sapiens
52 ECD  
Affinity Capture-MS Homo sapiens
53 Kcnma1  
Affinity Capture-MS Mus musculus
54 EPAS1  
Affinity Capture-Western Homo sapiens
55 POLR2E 5434
Affinity Capture-MS Homo sapiens
56 BRD8 10902
Affinity Capture-MS Homo sapiens
57 YY1 7528
Affinity Capture-MS Homo sapiens
58 TFPT  
Affinity Capture-MS Homo sapiens
59 EP400  
Affinity Capture-MS Homo sapiens
60 TSSC4  
Affinity Capture-MS Homo sapiens
61 MORF4L1  
Affinity Capture-MS Homo sapiens
62 POLR2A 5430
Affinity Capture-MS Homo sapiens
63 POLR3E 55718
Affinity Capture-MS Homo sapiens
64 ACTR6  
Affinity Capture-MS Homo sapiens
65 DMAP1 55929
Affinity Capture-MS Homo sapiens
66 MRGBP  
Affinity Capture-MS Homo sapiens
67 ACTR5 79913
Affinity Capture-MS Homo sapiens
68 ATP5E 514
Affinity Capture-MS Homo sapiens
69 INO80B 83444
Affinity Capture-MS Homo sapiens
70 ZNHIT6 54680
Affinity Capture-MS Homo sapiens
71 INO80  
Affinity Capture-MS Homo sapiens
72 KAT5  
Affinity Capture-MS Homo sapiens
73 RPAP3 79657
Affinity Capture-MS Homo sapiens
74 ACTR8 93973
Affinity Capture-MS Homo sapiens
75 Eed  
Affinity Capture-MS Mus musculus
76 Fancd2  
Affinity Capture-MS Mus musculus
77 Ruvbl2 20174
Two-hybrid Mus musculus
78 RUVBL2 10856
Affinity Capture-MS Homo sapiens
79 HECTD4 283450
Affinity Capture-MS Homo sapiens
80 Wdr1 22388
Affinity Capture-MS Mus musculus
81 WDR92 116143
Affinity Capture-MS Homo sapiens
82 RPAP2  
Affinity Capture-MS Homo sapiens
83 Chaf1a  
Affinity Capture-MS Mus musculus
84 EPC1  
Affinity Capture-MS Homo sapiens
85 STK26 51765
Affinity Capture-MS Homo sapiens
86 SHMT2 6472
Affinity Capture-MS Homo sapiens
87 TUBB4A 10382
Affinity Capture-MS Homo sapiens
88 INO80C  
Affinity Capture-MS Homo sapiens
89 TTI2 80185
Affinity Capture-MS Homo sapiens
90 SUPT6H 6830
Affinity Capture-MS Homo sapiens
91 YBX3 8531
Affinity Capture-MS Homo sapiens
92 IBTK 25998
Affinity Capture-MS Homo sapiens
93 POLR3D  
Affinity Capture-MS Homo sapiens
94 POLR3B 55703
Affinity Capture-MS Homo sapiens
95 C12orf45  
Affinity Capture-MS Homo sapiens
96 Zbtb7b  
Affinity Capture-MS Mus musculus
97 PIH1D1  
Affinity Capture-MS Homo sapiens
98 Ctr9  
Affinity Capture-MS Mus musculus
99 PDRG1  
Affinity Capture-MS Homo sapiens
100 ZNHIT3  
Affinity Capture-MS Homo sapiens
101 EPC2  
Affinity Capture-MS Homo sapiens
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