Gene description for HECTD4
Gene name HECT domain containing E3 ubiquitin protein ligase 4
Gene symbol HECTD4
Other names/aliases C12orf51
POTAGE
Species Homo sapiens
 Database cross references - HECTD4
ExoCarta ExoCarta_283450
Vesiclepedia VP_283450
Entrez Gene 283450
HGNC 26611
 HECTD4 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for HECTD4
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IEA
    protein binding GO:0005515 IPI
Biological Process
    glucose metabolic process GO:0006006 IMP
    protein ubiquitination GO:0016567 IEA
    glucose homeostasis GO:0042593 IBA
    glucose homeostasis GO:0042593 IMP
Subcellular Localization
    membrane GO:0016020 IEA
 Experiment description of studies that identified HECTD4 in sEVs
1
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for HECTD4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 SLFN11 91607
Affinity Capture-MS Homo sapiens
3 MECP2 4204
Affinity Capture-MS Homo sapiens
4 GRIA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SIRT6  
Affinity Capture-MS Homo sapiens
6 Ruvbl1 56505
Affinity Capture-MS Mus musculus
7 RPL7A 6130
Cross-Linking-MS (XL-MS) Homo sapiens
8 YWHAZ 7534
Affinity Capture-MS Homo sapiens
9 RABEP2 79874
Affinity Capture-MS Homo sapiens
10 YWHAB 7529
Affinity Capture-MS Homo sapiens
11 UBE2L3 7332
Reconstituted Complex Homo sapiens
12 VAMP4 8674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 KIF20A 10112
Affinity Capture-MS Homo sapiens
14 Shoc2  
Affinity Capture-MS Mus musculus
15 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 UBE2D2 7322
Reconstituted Complex Homo sapiens
17 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
18 MYC  
Affinity Capture-MS Homo sapiens
19 Pafah1b1 18472
Affinity Capture-MS Mus musculus
20 MAGEA4 4103
Affinity Capture-MS Homo sapiens
21 RPS8 6202
Cross-Linking-MS (XL-MS) Homo sapiens
22 RPS25 6230
Cross-Linking-MS (XL-MS) Homo sapiens
23 RPL36 25873
Cross-Linking-MS (XL-MS) Homo sapiens
24 YWHAQ 10971
Affinity Capture-MS Homo sapiens
25 UNC119 9094
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 Bub1b 12236
Affinity Capture-MS Mus musculus
27 AR 367
Affinity Capture-Western Homo sapiens
28 PGAM1 5223
Cross-Linking-MS (XL-MS) Homo sapiens
29 YWHAG 7532
Affinity Capture-MS Homo sapiens
30 Pdlim5 56376
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which HECTD4 is involved
No pathways found





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