Gene description for Dkc1
Gene name dyskeratosis congenita 1, dyskerin
Gene symbol Dkc1
Other names/aliases BC068171
Species Mus musculus
 Database cross references - Dkc1
ExoCarta ExoCarta_245474
Vesiclepedia VP_245474
Entrez Gene 245474
UniProt Q9ESX5  
 Dkc1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dkc1
Molecular Function
    telomerase activity GO:0003720 IEA
    telomerase activity GO:0003720 ISO
    telomerase activity GO:0003720 ISO
    RNA binding GO:0003723 ISO
    protein binding GO:0005515 IPI
    pseudouridine synthase activity GO:0009982 IBA
    pseudouridine synthase activity GO:0009982 ISO
    pseudouridine synthase activity GO:0009982 ISS
    box H/ACA snoRNA binding GO:0034513 IEA
    box H/ACA snoRNA binding GO:0034513 ISO
    telomerase RNA binding GO:0070034 IEA
    telomerase RNA binding GO:0070034 ISO
Biological Process
    snoRNA guided rRNA pseudouridine synthesis GO:0000454 ISO
    enzyme-directed rRNA pseudouridine synthesis GO:0000455 ISO
    enzyme-directed rRNA pseudouridine synthesis GO:0000455 ISS
    box H/ACA sno(s)RNA 3'-end processing GO:0000495 IBA
    telomere maintenance via telomerase GO:0007004 ISO
    telomere maintenance via telomerase GO:0007004 ISS
    telomere maintenance via telomerase GO:0007004 NAS
    positive regulation of cell population proliferation GO:0008284 ISO
    rRNA pseudouridine synthesis GO:0031118 IBA
    rRNA pseudouridine synthesis GO:0031118 IMP
    snRNA pseudouridine synthesis GO:0031120 IBA
    positive regulation of telomere maintenance via telomerase GO:0032212 IMP
    box H/ACA sno(s)RNA metabolic process GO:0033979 IMP
    scaRNA localization to Cajal body GO:0090666 IEA
    scaRNA localization to Cajal body GO:0090666 ISO
    telomerase RNA stabilization GO:0090669 IEA
    telomerase RNA stabilization GO:0090669 ISO
    positive regulation of establishment of protein localization to telomere GO:1904851 ISO
    regulation of telomerase RNA localization to Cajal body GO:1904872 ISO
    positive regulation of telomerase RNA localization to Cajal body GO:1904874 IEA
    positive regulation of telomerase RNA localization to Cajal body GO:1904874 ISO
    telomerase holoenzyme complex assembly GO:1905323 IMP
    mRNA pseudouridine synthesis GO:1990481 IBA
Subcellular Localization
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    telomerase holoenzyme complex GO:0005697 ISO
    telomerase holoenzyme complex GO:0005697 ISS
    telomerase holoenzyme complex GO:0005697 NAS
    nucleolus GO:0005730 IDA
    Cajal body GO:0015030 IDA
    Cajal body GO:0015030 ISO
    box H/ACA snoRNP complex GO:0031429 IBA
    box H/ACA snoRNP complex GO:0031429 IEA
    box H/ACA snoRNP complex GO:0031429 ISO
    box H/ACA telomerase RNP complex GO:0090661 IEA
    box H/ACA telomerase RNP complex GO:0090661 ISO
 Experiment description of studies that identified Dkc1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dkc1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dhx15 13204
Co-fractionation Mus musculus
2 Wdr12  
Co-fractionation Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Ruvbl2 20174
Co-fractionation Mus musculus
5 Drg1  
Co-fractionation Mus musculus
6 Prps1 19139
Co-fractionation Mus musculus
7 Mettl1  
Co-fractionation Mus musculus
8 Prps2 110639
Co-fractionation Mus musculus
9 Fancd2  
Affinity Capture-MS Mus musculus
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