Gene description for Dhx15
Gene name DEAH (Asp-Glu-Ala-His) box polypeptide 15
Gene symbol Dhx15
Other names/aliases DBP1
DEAH9
Ddx15
HRH2
mDEAH9
Species Mus musculus
 Database cross references - Dhx15
ExoCarta ExoCarta_13204
Vesiclepedia VP_13204
Entrez Gene 13204
UniProt O35286  
 Dhx15 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dhx15
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 ISO
    RNA helicase activity GO:0003724 ISO
    RNA helicase activity GO:0003724 ISS
    double-stranded RNA binding GO:0003725 ISO
    double-stranded RNA binding GO:0003725 ISS
    helicase activity GO:0004386 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    response to toxic substance GO:0009636 IEA
    defense response to bacterium GO:0042742 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISS
    response to alkaloid GO:0043279 IEA
    defense response to virus GO:0051607 IDA
    defense response to virus GO:0051607 IMP
    defense response to virus GO:0051607 ISO
    antiviral innate immune response GO:0140374 IDA
    antiviral innate immune response GO:0140374 IMP
    antiviral innate immune response GO:0140374 ISO
    chromatin looping GO:0140588 IEA
Subcellular Localization
    spliceosomal complex GO:0005681 IBA
    U12-type spliceosomal complex GO:0005689 ISO
    U12-type spliceosomal complex GO:0005689 ISS
    nucleolus GO:0005730 IEA
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    U2-type post-mRNA release spliceosomal complex GO:0071008 IEA
    U2-type post-mRNA release spliceosomal complex GO:0071008 ISO
 Experiment description of studies that identified Dhx15 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dhx15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 Wdr12  
Co-fractionation Mus musculus
3 Trmt11  
Co-fractionation Mus musculus
4 Dkc1 245474
Co-fractionation Mus musculus
5 Snrpd1 20641
Co-fractionation Mus musculus
6 Prpf8 192159
Co-fractionation Mus musculus
7 Nle1  
Co-fractionation Mus musculus
8 Pak1ip1  
Co-fractionation Mus musculus
9 Wbscr22  
Co-fractionation Mus musculus
10 Sf3b1 81898
Co-fractionation Mus musculus
11 Snrnp200 320632
Co-fractionation Mus musculus
12 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
13 Kctd13  
Affinity Capture-MS Mus musculus
14 Rsl1d1 66409
Co-fractionation Mus musculus
15 Snrpd2 107686
Co-fractionation Mus musculus
16 UBC 7316
Affinity Capture-Western Homo sapiens
17 Rps11 27207
Co-fractionation Mus musculus
18 Rpia  
Co-fractionation Mus musculus
19 Pabpc1 18458
Co-fractionation Mus musculus
20 Snrpd3 67332
Co-fractionation Mus musculus
21 Sf3b3 101943
Co-fractionation Mus musculus
22 Tardbp 230908
Co-fractionation Mus musculus
23 Rpl19 19921
Co-fractionation Mus musculus
24 Puf60 67959
Co-fractionation Mus musculus
25 Unk  
Affinity Capture-RNA Mus musculus
26 3110082I17Rik  
Co-fractionation Mus musculus
27 Hnrnpdl 50926
Co-fractionation Mus musculus
28 Ctps2  
Co-fractionation Mus musculus
29 Ddx42 72047
Co-fractionation Mus musculus
30 Eed  
Affinity Capture-MS Mus musculus
31 U2af2 22185
Co-fractionation Mus musculus
32 Rps16 20055
Co-fractionation Mus musculus
33 Fancd2  
Affinity Capture-MS Mus musculus
34 Pwp1  
Co-fractionation Mus musculus
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