Gene description for Puf60
Gene name poly-U binding splicing factor 60
Gene symbol Puf60
Other names/aliases 2410104I19Rik
2810454F19Rik
SIAHBP1
Species Mus musculus
 Database cross references - Puf60
ExoCarta ExoCarta_67959
Vesiclepedia VP_67959
Entrez Gene 67959
UniProt Q3UEB3  
 Puf60 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Puf60
Molecular Function
    DNA binding GO:0003677 IEA
    RNA binding GO:0003723 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    alternative mRNA splicing, via spliceosome GO:0000380 IBA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IBA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IEA
    mRNA splice site recognition GO:0006376 IBA
    mRNA processing GO:0006397 IEA
    apoptotic process GO:0006915 IEA
    RNA splicing GO:0008380 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cell junction GO:0030054 IEA
    cell junction GO:0030054 ISO
    ribonucleoprotein complex GO:1990904 IEA
 Experiment description of studies that identified Puf60 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔
Cd81|Icam1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Puf60
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kcna3  
Affinity Capture-MS Mus musculus
2 Sf3a3 75062
Co-fractionation Mus musculus
3 Dhx15 13204
Co-fractionation Mus musculus
4 JADE1  
Affinity Capture-MS Homo sapiens
5 Sf3b1 81898
Co-fractionation Mus musculus
6 Tmem173  
Proximity Label-MS Mus musculus
7 Faf1  
Co-fractionation Mus musculus
8 2810403A07Rik  
Co-fractionation Mus musculus
9 Eed  
Affinity Capture-MS Mus musculus
10 Sf3b3 101943
Co-fractionation Mus musculus
11 Srsf1 110809
Co-fractionation Mus musculus
12 Ptbp2  
Co-fractionation Mus musculus
13 U2af2 22185
Co-fractionation Mus musculus
14 Snrpd2 107686
Co-fractionation Mus musculus
15 HIST1H3A 8350
Affinity Capture-MS Homo sapiens
16 Fancd2  
Affinity Capture-MS Mus musculus
17 Srrm1 51796
Co-fractionation Mus musculus
18 Sf3b6  
Co-fractionation Mus musculus
19 Snrpd3 67332
Co-fractionation Mus musculus
20 Ugp2 216558
Co-fractionation Mus musculus
View the network image/svg+xml



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