Gene description for Sf3b1
Gene name splicing factor 3b, subunit 1
Gene symbol Sf3b1
Other names/aliases 155kDa
2810001M05Rik
AA409119
Prp10
SAP155
SF3b155
TA-8
Targ4
Species Mus musculus
 Database cross references - Sf3b1
ExoCarta ExoCarta_81898
Vesiclepedia VP_81898
Entrez Gene 81898
 Sf3b1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sf3b1
Molecular Function
    chromatin binding GO:0003682 IDA
    RNA binding GO:0003723 ISO
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 IEA
    protein binding GO:0005515 IPI
    splicing factor binding GO:1990935 ISO
Biological Process
    spliceosomal complex assembly GO:0000245 IBA
    spliceosomal complex assembly GO:0000245 IEA
    mRNA splicing, via spliceosome GO:0000398 IMP
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    blastocyst formation GO:0001825 IMP
    chromatin remodeling GO:0006338 NAS
    anterior/posterior pattern specification GO:0009952 IGI
    positive regulation of transcription by RNA polymerase I GO:0045943 NAS
    positive regulation of transcription by RNA polymerase II GO:0045944 NAS
    positive regulation of transcription by RNA polymerase III GO:0045945 ISO
Subcellular Localization
    chromatin GO:0000785 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    U2-type spliceosomal complex GO:0005684 ISO
    U2-type spliceosomal complex GO:0005684 ISS
    U2 snRNP GO:0005686 IBA
    U2 snRNP GO:0005686 ISO
    U12-type spliceosomal complex GO:0005689 IBA
    U12-type spliceosomal complex GO:0005689 ISO
    nucleolus GO:0005730 NAS
    nuclear matrix GO:0016363 IDA
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    small nuclear ribonucleoprotein complex GO:0030532 IDA
    U11/U12 snRNP GO:0034693 ISO
    U2-type prespliceosome GO:0071004 IBA
    U2-type precatalytic spliceosome GO:0071005 ISO
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 ISO
    B-WICH complex GO:0110016 ISO
 Experiment description of studies that identified Sf3b1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sf3b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ddx5 13207
Co-fractionation Mus musculus
2 Hspd1 15510
Co-fractionation Mus musculus
3 Fancd2  
Affinity Capture-MS Mus musculus
4 Mycbp2 105689
Affinity Capture-MS Mus musculus
5 Hspa8 15481
Co-fractionation Mus musculus
6 Snrpd1 20641
Co-fractionation Mus musculus
7 Srrm1 51796
Co-fractionation Mus musculus
8 Cdc5l 71702
Co-fractionation Mus musculus
9 Plrg1  
Co-fractionation Mus musculus
10 Alyref  
Co-fractionation Mus musculus
11 Cul3  
Affinity Capture-MS Mus musculus
12 Ybx1 22608
Co-fractionation Mus musculus
13 Kctd13  
Affinity Capture-MS Mus musculus
14 Hnrnpu 51810
Co-fractionation Mus musculus
15 Prpf19 28000
Co-fractionation Mus musculus
16 Rbm8a  
Co-fractionation Mus musculus
17 Sf3b3 101943
Co-fractionation Mus musculus
18 Mettl21e  
Affinity Capture-MS Mus musculus
19 Snrpe 20643
Co-fractionation Mus musculus
20 Pabpc1 18458
Co-fractionation Mus musculus
21 Srrm2  
Co-fractionation Mus musculus
22 Puf60 67959
Co-fractionation Mus musculus
23 Unk  
Affinity Capture-RNA Mus musculus
24 Syncrip 56403
Co-fractionation Mus musculus
25 Ppwd1  
Co-fractionation Mus musculus
26 Sfpq 71514
Co-fractionation Mus musculus
27 Dhx15 13204
Co-fractionation Mus musculus
28 Hnrnpa2b1 53379
Co-fractionation Mus musculus
29 Hnrnpk 15387
Co-fractionation Mus musculus
30 Ddx42 72047
Co-fractionation Mus musculus
31 Eed  
Affinity Capture-MS Mus musculus
32 U2af2 22185
Co-fractionation Mus musculus
33 Skiv2l2  
Co-fractionation Mus musculus
34 Eftud2 20624
Co-fractionation Mus musculus
35 Ppie  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Sf3b1 is involved
No pathways found





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