Gene description for Prpf19
Gene name PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
Gene symbol Prpf19
Other names/aliases AA617263
AL024362
D19Wsu55e
NMP200
PSO4
Prp19
Snev
Species Mus musculus
 Database cross references - Prpf19
ExoCarta ExoCarta_28000
Vesiclepedia VP_28000
Entrez Gene 28000
UniProt Q99KP6  
 Prpf19 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prpf19
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IBA
    protein binding GO:0005515 IPI
    ubiquitin-ubiquitin ligase activity GO:0034450 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    ubiquitin protein ligase activity GO:0061630 ISO
    ubiquitin protein ligase activity GO:0061630 ISS
Biological Process
    DNA damage checkpoint signaling GO:0000077 ISO
    DNA damage checkpoint signaling GO:0000077 ISS
    protein polyubiquitination GO:0000209 ISO
    spliceosomal tri-snRNP complex assembly GO:0000244 ISO
    spliceosomal tri-snRNP complex assembly GO:0000244 ISS
    spliceosomal complex assembly GO:0000245 IEA
    spliceosomal complex assembly GO:0000245 ISO
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    mRNA splicing, via spliceosome GO:0000398 NAS
    double-strand break repair via nonhomologous end joining GO:0006303 ISO
    double-strand break repair via nonhomologous end joining GO:0006303 ISS
    protein localization GO:0008104 ISO
    protein localization GO:0008104 ISS
    lipid biosynthetic process GO:0008610 IDA
    proteasomal protein catabolic process GO:0010498 IMP
    proteasomal protein catabolic process GO:0010498 ISO
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IMP
    positive regulation of astrocyte differentiation GO:0048711 ISO
    protein K63-linked ubiquitination GO:0070534 ISO
    protein K63-linked ubiquitination GO:0070534 ISS
Subcellular Localization
    Prp19 complex GO:0000974 IBA
    Prp19 complex GO:0000974 ISO
    Prp19 complex GO:0000974 ISS
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    DNA replication factor A complex GO:0005662 ISO
    DNA replication factor A complex GO:0005662 ISS
    spliceosomal complex GO:0005681 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    lipid droplet GO:0005811 IEA
    spindle GO:0005819 IEA
    nuclear speck GO:0016607 ISO
    nuclear speck GO:0016607 ISS
    site of double-strand break GO:0035861 ISO
    site of double-strand break GO:0035861 ISS
    U2-type catalytic step 1 spliceosome GO:0071006 IBA
    U2-type catalytic step 2 spliceosome GO:0071007 IEA
    U2-type catalytic step 2 spliceosome GO:0071007 ISO
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Prpf19 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prpf19
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 Sf3b3 101943
Co-fractionation Mus musculus
3 Srrm1 51796
Co-fractionation Mus musculus
4 Prpf8 192159
Co-fractionation Mus musculus
5 Cdc5l 71702
Co-fractionation Mus musculus
6 Sf3b1 81898
Co-fractionation Mus musculus
7 Atxn1  
Affinity Capture-MS Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
9 Snrnp200 320632
Co-fractionation Mus musculus
10 Psmc5 19184
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
11 Mecp2  
Affinity Capture-MS Mus musculus
12 Prpf3 70767
Co-fractionation Mus musculus
13 Mettl21e  
Affinity Capture-MS Mus musculus
14 Fzr1  
Affinity Capture-MS Mus musculus
15 Ctnnbl1  
Co-fractionation Mus musculus
16 Srrm2  
Co-fractionation Mus musculus
17 Plrg1  
Co-fractionation Mus musculus
18 Zbtb7b  
Affinity Capture-MS Mus musculus
19 Fancd2  
Affinity Capture-MS Mus musculus
20 Ddx42 72047
Co-fractionation Mus musculus
21 Eed  
Affinity Capture-MS Mus musculus
22 Cwc27  
Co-fractionation Mus musculus
23 Skiv2l2  
Co-fractionation Mus musculus
24 Eftud2 20624
Co-fractionation Mus musculus
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