Gene description for Sf3a3
Gene name splicing factor 3a, subunit 3
Gene symbol Sf3a3
Other names/aliases 4930512K19Rik
60kDa
Species Mus musculus
 Database cross references - Sf3a3
ExoCarta ExoCarta_75062
Vesiclepedia VP_75062
Entrez Gene 75062
UniProt Q9D554  
 Sf3a3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sf3a3
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IEA
    zinc ion binding GO:0008270 IEA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    mRNA processing GO:0006397 ISO
    U2-type prespliceosome assembly GO:1903241 ISO
    U2-type prespliceosome assembly GO:1903241 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 IBA
    spliceosomal complex GO:0005681 ISO
    U2-type spliceosomal complex GO:0005684 ISO
    U2 snRNP GO:0005686 ISO
    U2 snRNP GO:0005686 ISS
    nuclear speck GO:0016607 ISO
    nuclear speck GO:0016607 ISS
    U2-type precatalytic spliceosome GO:0071005 ISO
    U2-type precatalytic spliceosome GO:0071005 ISS
    catalytic step 2 spliceosome GO:0071013 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Sf3a3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sf3a3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ddx5 13207
Co-fractionation Mus musculus
2 Dhx29  
Co-fractionation Mus musculus
3 Prpf8 192159
Co-fractionation Mus musculus
4 Cbwd1  
Co-fractionation Mus musculus
5 Lsm7 66094
Co-fractionation Mus musculus
6 Sf3b3 101943
Co-fractionation Mus musculus
7 SNW1 22938
Two-hybrid Homo sapiens
8 EXOSC7 23016
Two-hybrid Homo sapiens
9 Fancd2  
Affinity Capture-MS Mus musculus
10 Rpl22 19934
Co-fractionation Mus musculus
11 Hnrnpf 98758
Co-fractionation Mus musculus
12 Hypk  
Co-fractionation Mus musculus
13 Gpatch1  
Co-fractionation Mus musculus
14 MAZ 4150
Two-hybrid Homo sapiens
15 Hnrnpc 15381
Co-fractionation Mus musculus
16 Prpf4b 19134
Co-fractionation Mus musculus
17 Zmat2  
Co-fractionation Mus musculus
18 Klc4  
Two-hybrid Mus musculus
19 Pou5f1  
Affinity Capture-MS Mus musculus
20 Rps4x 20102
Co-fractionation Mus musculus
21 TFIP11  
Two-hybrid Homo sapiens
22 Cdc5l 71702
Co-fractionation Mus musculus
23 Ppil3  
Co-fractionation Mus musculus
24 Rps2 16898
Co-fractionation Mus musculus
25 Syncrip 56403
Co-fractionation Mus musculus
26 PRC1 9055
Two-hybrid Homo sapiens
27 U2af2 22185
Co-fractionation Mus musculus
28 Spen  
Co-fractionation Mus musculus
29 Rpl7a 27176
Co-fractionation Mus musculus
30 Snrnp200 320632
Co-fractionation Mus musculus
31 Foxp3  
Affinity Capture-MS Mus musculus
32 Prmt5 27374
Co-fractionation Mus musculus
33 UTP14A 10813
Two-hybrid Homo sapiens
34 Trim69  
Co-fractionation Mus musculus
35 Dnajc8  
Co-fractionation Mus musculus
36 Hnrnpu 51810
Co-fractionation Mus musculus
37 Frg1  
Co-fractionation Mus musculus
38 Prpf19 28000
Co-fractionation Mus musculus
39 Snrpd2 107686
Co-fractionation Mus musculus
40 Chaf1a  
Affinity Capture-MS Mus musculus
41 Prpf40a  
Co-fractionation Mus musculus
42 Snrnp27  
Co-fractionation Mus musculus
43 Ddx17 67040
Co-fractionation Mus musculus
44 CPSF7 79869
Two-hybrid Homo sapiens
45 Snrpd3 67332
Co-fractionation Mus musculus
46 Rpl35a 57808
Co-fractionation Mus musculus
47 Lrrc59  
Co-fractionation Mus musculus
48 Prpf3 70767
Co-fractionation Mus musculus
49 Pnn  
Co-fractionation Mus musculus
50 Cwc15  
Co-fractionation Mus musculus
51 2310036O22Rik  
Co-fractionation Mus musculus
52 Gtf2f1  
Co-fractionation Mus musculus
53 Ctnnbl1  
Co-fractionation Mus musculus
54 Plod3 26433
Co-fractionation Mus musculus
55 Rcsd1  
Co-fractionation Mus musculus
56 Srrm2  
Co-fractionation Mus musculus
57 Hnrnpa3 229279
Co-fractionation Mus musculus
58 Puf60 67959
Co-fractionation Mus musculus
59 Tfip11  
Co-fractionation Mus musculus
60 Plrg1  
Co-fractionation Mus musculus
61 Snw1  
Co-fractionation Mus musculus
62 Rps3a1 20091
Co-fractionation Mus musculus
63 Ppwd1  
Co-fractionation Mus musculus
64 Wbp4  
Co-fractionation Mus musculus
65 Dhx15 13204
Co-fractionation Mus musculus
66 Hnrnpm 76936
Co-fractionation Mus musculus
67 Snrpa1 68981
Co-fractionation Mus musculus
68 Rps14 20044
Co-fractionation Mus musculus
69 Hnrnpk 15387
Co-fractionation Mus musculus
70 Smndc1  
Co-fractionation Mus musculus
71 Bbs5  
Two-hybrid Mus musculus
72 Cwc27  
Co-fractionation Mus musculus
73 Rpl15 66480
Co-fractionation Mus musculus
74 Top1 21969
Co-fractionation Mus musculus
75 Skiv2l2  
Co-fractionation Mus musculus
76 Eif4a3 192170
Co-fractionation Mus musculus
77 PRPF31 26121
Two-hybrid Homo sapiens
78 Thoc1  
Co-fractionation Mus musculus
79 DDX49 54555
Two-hybrid Homo sapiens
80 Rpl6 19988
Co-fractionation Mus musculus
81 Eftud2 20624
Co-fractionation Mus musculus
82 Paxbp1  
Co-fractionation Mus musculus
83 Hnrnpa2b1 53379
Co-fractionation Mus musculus
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