Gene description for Hnrnpu
Gene name heterogeneous nuclear ribonucleoprotein U
Gene symbol Hnrnpu
Other names/aliases AA408410
AI256620
AL024194
AL024437
AW557595
C86794
Hnrpu
SAFA
Sp120
hnRNP U
Species Mus musculus
 Database cross references - Hnrnpu
ExoCarta ExoCarta_51810
Vesiclepedia VP_51810
Entrez Gene 51810
UniProt Q8VEK3  
 Hnrnpu identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hnrnpu
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    RNA polymerase II complex binding GO:0000993 ISO
    RNA polymerase II complex binding GO:0000993 ISS
    TFIIH-class transcription factor complex binding GO:0001097 ISO
    TFIIH-class transcription factor complex binding GO:0001097 ISS
    DNA binding GO:0003677 ISO
    chromatin binding GO:0003682 ISO
    chromatin binding GO:0003682 ISS
    double-stranded DNA binding GO:0003690 ISO
    double-stranded DNA binding GO:0003690 ISS
    single-stranded DNA binding GO:0003697 ISO
    single-stranded DNA binding GO:0003697 ISS
    transcription corepressor activity GO:0003714 ISO
    transcription corepressor activity GO:0003714 ISS
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IDA
    RNA binding GO:0003723 IPI
    RNA binding GO:0003723 ISO
    double-stranded RNA binding GO:0003725 ISO
    double-stranded RNA binding GO:0003725 ISS
    single-stranded RNA binding GO:0003727 ISO
    single-stranded RNA binding GO:0003727 ISS
    mRNA 3'-UTR binding GO:0003730 ISO
    mRNA 3'-UTR binding GO:0003730 ISS
    actin binding GO:0003779 ISO
    actin binding GO:0003779 ISS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 ISO
    ATP binding GO:0005524 ISS
    poly(A) binding GO:0008143 ISO
    poly(A) binding GO:0008143 ISS
    snRNA binding GO:0017069 ISO
    snRNA binding GO:0017069 ISS
    poly(C) RNA binding GO:0017130 ISO
    poly(C) RNA binding GO:0017130 ISS
    chromatin DNA binding GO:0031490 ISO
    chromatin DNA binding GO:0031490 ISS
    poly(G) binding GO:0034046 ISO
    poly(G) binding GO:0034046 ISS
    piRNA binding GO:0034584 IEA
    pre-mRNA binding GO:0036002 ISO
    pre-mRNA binding GO:0036002 ISS
    identical protein binding GO:0042802 ISO
    identical protein binding GO:0042802 ISS
    ribonucleoprotein complex binding GO:0043021 IEA
    ribonucleoprotein complex binding GO:0043021 ISO
    sequence-specific DNA binding GO:0043565 IDA
    protein-containing complex binding GO:0044877 ISO
    protein-containing complex binding GO:0044877 ISS
    telomerase RNA binding GO:0070034 IEA
    telomerase RNA binding GO:0070034 ISO
    RNA polymerase II C-terminal domain binding GO:0099122 IDA
    lncRNA binding GO:0106222 IDA
    sequence-specific double-stranded DNA binding GO:1990837 ISO
    sequence-specific double-stranded DNA binding GO:1990837 ISS
    promoter-specific chromatin binding GO:1990841 IBA
    promoter-specific chromatin binding GO:1990841 IDA
    promoter-specific chromatin binding GO:1990841 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    alternative mRNA splicing, via spliceosome GO:0000380 IBA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISO
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISS
    mRNA processing GO:0006397 IEA
    regulation of mitotic cell cycle GO:0007346 ISO
    regulation of mitotic cell cycle GO:0007346 ISS
    RNA splicing GO:0008380 IEA
    dosage compensation by inactivation of X chromosome GO:0009048 IMP
    positive regulation of gene expression GO:0010628 ISO
    mRNA metabolic process GO:0016071 IMP
    erythrocyte differentiation GO:0030218 IMP
    regulatory ncRNA-mediated heterochromatin formation GO:0031048 IEA
    negative regulation of telomere maintenance via telomerase GO:0032211 IEA
    negative regulation of telomere maintenance via telomerase GO:0032211 ISO
    circadian regulation of gene expression GO:0032922 IDA
    negative regulation of kinase activity GO:0033673 ISO
    negative regulation of kinase activity GO:0033673 ISS
    negative regulation of transcription elongation by RNA polymerase II GO:0034244 ISO
    negative regulation of transcription elongation by RNA polymerase II GO:0034244 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    mRNA stabilization GO:0048255 ISO
    mRNA stabilization GO:0048255 ISS
    cell division GO:0051301 IEA
    maintenance of protein location in nucleus GO:0051457 ISO
    maintenance of protein location in nucleus GO:0051457 ISS
    cardiac muscle cell development GO:0055013 IMP
    random inactivation of X chromosome GO:0060816 IMP
    CRD-mediated mRNA stabilization GO:0070934 ISO
    cellular response to glucocorticoid stimulus GO:0071385 ISO
    cellular response to glucocorticoid stimulus GO:0071385 ISS
    cellular response to dexamethasone stimulus GO:0071549 ISO
    positive regulation of brown fat cell differentiation GO:0090336 IMP
    dendritic transport of messenger ribonucleoprotein complex GO:0098963 IDA
    dendritic transport of messenger ribonucleoprotein complex GO:0098963 IMP
    negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900152 ISO
    regulation of mitotic spindle assembly GO:1901673 ISO
    regulation of mitotic spindle assembly GO:1901673 ISS
    regulation of chromatin organization GO:1902275 ISO
    regulation of chromatin organization GO:1902275 ISS
    positive regulation of attachment of mitotic spindle microtubules to kinetochore GO:1902425 ISO
    positive regulation of attachment of mitotic spindle microtubules to kinetochore GO:1902425 ISS
    protein localization to spindle microtubule GO:1902889 ISO
    protein localization to spindle microtubule GO:1902889 ISS
    RNA localization to chromatin GO:1990280 IMP
    cellular response to leukemia inhibitory factor GO:1990830 IDA
    adaptive thermogenesis GO:1990845 IMP
    positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity GO:2000373 ISS
    positive regulation of stem cell proliferation GO:2000648 IMP
    negative regulation of stem cell differentiation GO:2000737 IMP
    negative regulation of stem cell differentiation GO:2000737 ISO
    positive regulation of cytoplasmic translation GO:2000767 ISO
Subcellular Localization
    nuclear chromosome GO:0000228 ISO
    nuclear chromosome GO:0000228 ISS
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISS
    spindle pole GO:0000922 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 TAS
    telomerase holoenzyme complex GO:0005697 IEA
    telomerase holoenzyme complex GO:0005697 ISO
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    cytosol GO:0005829 ISO
    cell surface GO:0009986 ISO
    cell surface GO:0009986 ISS
    nuclear matrix GO:0016363 ISO
    nuclear matrix GO:0016363 ISS
    nuclear speck GO:0016607 ISO
    nuclear speck GO:0016607 ISS
    dendrite GO:0030425 IEA
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    protein-containing complex GO:0032991 ISO
    protein-containing complex GO:0032991 ISS
    cytoplasmic ribonucleoprotein granule GO:0036464 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    CRD-mediated mRNA stability complex GO:0070937 ISO
    catalytic step 2 spliceosome GO:0071013 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
    mitotic spindle GO:0072686 ISO
    mitotic spindle GO:0072686 ISS
    RNA polymerase II transcription regulator complex GO:0090575 ISO
    RNA polymerase II transcription regulator complex GO:0090575 ISS
    inactive sex chromosome GO:0098577 IDA
    inactive sex chromosome GO:0098577 ISO
    inactive sex chromosome GO:0098577 ISS
    mitotic spindle midzone GO:1990023 ISO
    mitotic spindle midzone GO:1990023 ISS
    mitotic spindle microtubule GO:1990498 ISO
    mitotic spindle microtubule GO:1990498 ISS
    ribonucleoprotein complex GO:1990904 IBA
    ribonucleoprotein complex GO:1990904 IDA
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Hnrnpu in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hnrnpu
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 Snw1  
Co-fractionation Mus musculus
3 Fxr1 14359
Affinity Capture-MS Mus musculus
4 Prpf8 192159
Co-fractionation Mus musculus
5 Ncbp1 433702
Co-fractionation Mus musculus
6 Unk  
Affinity Capture-RNA Mus musculus
7 MKRN1 23608
Affinity Capture-MS Homo sapiens
8 Rpl13a 22121
Co-fractionation Mus musculus
9 Atxn2 20239
Co-fractionation Mus musculus
10 Sarnp 66118
Co-fractionation Mus musculus
11 Hnrnpc 15381
Co-fractionation Mus musculus
12 Stau1  
Co-fractionation Mus musculus
13 Pou5f1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
14 Cbx1  
Affinity Capture-MS Mus musculus
15 Cdc5l 71702
Co-fractionation Mus musculus
16 Hnrnpl 15388
Co-fractionation Mus musculus
17 Sf3b1 81898
Co-fractionation Mus musculus
18 2700060E02Rik 68045
Co-fractionation Mus musculus
19 Snrnp200 320632
Co-fractionation Mus musculus
20 Fmr1  
Co-fractionation Mus musculus
21 Rbm39 170791
Co-fractionation Mus musculus
22 Kctd13  
Affinity Capture-MS Mus musculus
23 Tfe3  
Affinity Capture-MS Mus musculus
24 Cul3  
Affinity Capture-MS Mus musculus
25 Frg1  
Co-fractionation Mus musculus
26 Sf3a1 67465
Co-fractionation Mus musculus
27 Ago1 236511
Co-fractionation Mus musculus
28 Sf3b3 101943
Co-fractionation Mus musculus
29 Cbx5  
Affinity Capture-MS Mus musculus
30 Rbm8a  
Co-fractionation Mus musculus
31 Tmem173  
Proximity Label-MS Mus musculus
32 Fzr1  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
33 Tardbp 230908
Co-fractionation Mus musculus
34 Srsf1 110809
Co-fractionation Mus musculus
35 MAPT  
Reconstituted Complex Homo sapiens
36 Rock2  
Co-fractionation Mus musculus
37 Ago2 239528
Co-fractionation Mus musculus
38 Atg12  
Negative Genetic Mus musculus
39 RBM3 5935
Two-hybrid Homo sapiens
40 Ina  
Co-fractionation Mus musculus
41 Plrg1  
Co-fractionation Mus musculus
42 Prmt1 15469
Affinity Capture-MS Mus musculus
43 Cbx3 12417
Affinity Capture-MS Mus musculus
44 Ppwd1  
Co-fractionation Mus musculus
45 Sfpq 71514
Co-fractionation Mus musculus
46 Poldip3  
Co-fractionation Mus musculus
47 Whsc1  
Affinity Capture-MS Mus musculus
48 Hnrnpk 15387
Two-hybrid Mus musculus
49 Ppil3  
Co-fractionation Mus musculus
50 Eed  
Affinity Capture-MS Mus musculus
51 Cwc27  
Co-fractionation Mus musculus
52 Mapt  
Affinity Capture-MS Mus musculus
53 Skiv2l2  
Co-fractionation Mus musculus
54 Polr2a  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
55 Fancd2  
Affinity Capture-MS Mus musculus
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