Gene description for Rpl13a
Gene name ribosomal protein L13A
Gene symbol Rpl13a
Other names/aliases 1810026N22Rik
Tstap198-7
tum-antigen
Species Mus musculus
 Database cross references - Rpl13a
ExoCarta ExoCarta_22121
Vesiclepedia VP_22121
Entrez Gene 22121
UniProt P19253  
 Rpl13a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl13a
Molecular Function
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 IDA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
Biological Process
    cytoplasmic translation GO:0002181 NAS
    negative regulation of translation GO:0017148 IBA
    negative regulation of translation GO:0017148 IDA
    negative regulation of translation GO:0017148 ISO
    response to lipopolysaccharide GO:0032496 IMP
    homeostatic process GO:0042592 IMP
    macrophage chemotaxis GO:0048246 IMP
    lung morphogenesis GO:0060425 IMP
    cellular response to type II interferon GO:0071346 IDA
    cellular response to type II interferon GO:0071346 ISO
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
    negative regulation of formation of translation preinitiation complex GO:1901194 IEA
    negative regulation of formation of translation preinitiation complex GO:1901194 ISO
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 IBA
    ribosome GO:0005840 ISO
    ribosome GO:0005840 NAS
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    GAIT complex GO:0097452 IDA
    GAIT complex GO:0097452 ISO
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
    ribonucleoprotein complex GO:1990904 IDA
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Rpl13a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl13a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Herc4  
Co-fractionation Mus musculus
3 Lars 107045
Co-fractionation Mus musculus
4 Atxn2 20239
Co-fractionation Mus musculus
5 Rpl27 19942
Co-fractionation Mus musculus
6 Ybx1 22608
Co-fractionation Mus musculus
7 Fmr1  
Co-fractionation Mus musculus
8 Rpl6 19988
Co-fractionation Mus musculus
9 Hnrnpu 51810
Co-fractionation Mus musculus
10 Rps6 20104
Co-fractionation Mus musculus
11 Ncl 17975
Co-fractionation Mus musculus
12 Rpl35a 57808
Co-fractionation Mus musculus
13 Tmem173  
Proximity Label-MS Mus musculus
14 Hnrnpd 11991
Co-fractionation Mus musculus
15 Rpl7 19989
Co-fractionation Mus musculus
16 Rps9 76846
Co-fractionation Mus musculus
17 Sra1  
Co-fractionation Mus musculus
18 Rpl36a 19982
Co-fractionation Mus musculus
19 Rpl26 19941
Co-fractionation Mus musculus
20 Sstr3  
Proximity Label-MS Mus musculus
21 Eprs 107508
Co-fractionation Mus musculus
22 Rpsa 16785
Co-fractionation Mus musculus
23 Abce1 24015
Co-fractionation Mus musculus
24 Dock11  
Co-fractionation Mus musculus
25 Rpl11 67025
Co-fractionation Mus musculus
26 Eed  
Affinity Capture-MS Mus musculus
27 Rpl31 114641
Co-fractionation Mus musculus
28 Rps10 67097
Co-fractionation Mus musculus
29 Eif5a 276770
Co-fractionation Mus musculus
30 Rplp2 67186
Co-fractionation Mus musculus
31 Rps4x 20102
Co-fractionation Mus musculus
32 Trove2 20822
Co-fractionation Mus musculus
33 Rps23 66475
Co-fractionation Mus musculus
34 Scyl2  
Co-fractionation Mus musculus
35 Tmem165  
Co-fractionation Mus musculus
36 Rpl24 68193
Co-fractionation Mus musculus
37 Rps7 20115
Co-fractionation Mus musculus
38 Rpl17 319195
Co-fractionation Mus musculus
39 Uggt1 320011
Co-fractionation Mus musculus
40 Gtf2f1  
Co-fractionation Mus musculus
41 Actn4 60595
Co-fractionation Mus musculus
42 Rps15a 267019
Co-fractionation Mus musculus
43 Rplp0 11837
Co-fractionation Mus musculus
44 Rpl30 19946
Co-fractionation Mus musculus
45 Rpl39 67248
Co-fractionation Mus musculus
46 Rps16 20055
Co-fractionation Mus musculus
47 Rps28 54127
Co-fractionation Mus musculus
48 Dhx29  
Co-fractionation Mus musculus
49 Rps18 20084
Co-fractionation Mus musculus
50 Rpl14 67115
Co-fractionation Mus musculus
51 Rps2 16898
Co-fractionation Mus musculus
52 Gapdh 14433
Co-fractionation Mus musculus
53 Rps8 20116
Co-fractionation Mus musculus
54 Rpl7a 27176
Co-fractionation Mus musculus
55 Pde12  
Co-fractionation Mus musculus
56 Kcna3  
Affinity Capture-MS Mus musculus
57 Rps19 20085
Co-fractionation Mus musculus
58 Tpt1 22070
Co-fractionation Mus musculus
59 Rps11 27207
Co-fractionation Mus musculus
60 Hnrnpab 15384
Co-fractionation Mus musculus
61 Lrrc59  
Co-fractionation Mus musculus
62 Rpl22 19934
Co-fractionation Mus musculus
63 Tardbp 230908
Co-fractionation Mus musculus
64 Ago2 239528
Co-fractionation Mus musculus
65 Dhx9 13211
Co-fractionation Mus musculus
66 Syncrip 56403
Co-fractionation Mus musculus
67 Rps3a1 20091
Co-fractionation Mus musculus
68 Rps21 66481
Co-fractionation Mus musculus
69 Rps14 20044
Co-fractionation Mus musculus
70 Rpl35 66489
Co-fractionation Mus musculus
71 Tia1  
Affinity Capture-MS Mus musculus
72 Rpl5 100503670
Co-fractionation Mus musculus
73 Fancd2  
Affinity Capture-MS Mus musculus
74 Rps27a 78294
Co-fractionation Mus musculus
75 Ubr4 69116
Co-fractionation Mus musculus
76 Ncbp1 433702
Co-fractionation Mus musculus
77 Rps25 75617
Co-fractionation Mus musculus
78 Rpl10a 19896
Co-fractionation Mus musculus
79 Top1 21969
Co-fractionation Mus musculus
80 Kctd13  
Affinity Capture-MS Mus musculus
81 Ccdc55  
Co-fractionation Mus musculus
82 Prpf3 70767
Co-fractionation Mus musculus
83 Fzr1  
Affinity Capture-MS Mus musculus
84 Eef2 13629
Co-fractionation Mus musculus
85 Rpl12 269261
Co-fractionation Mus musculus
86 Rpl18 19899
Co-fractionation Mus musculus
87 Gnb2l1 14694
Co-fractionation Mus musculus
88 Npm1 18148
Affinity Capture-MS Mus musculus
89 Rps13 68052
Co-fractionation Mus musculus
90 Tcf25  
Co-fractionation Mus musculus
91 Rpl23 65019
Co-fractionation Mus musculus
92 Rpl23a 268449
Co-fractionation Mus musculus
93 Ilf3 16201
Co-fractionation Mus musculus
94 Rpl15 66480
Co-fractionation Mus musculus
95 Eef1b2 55949
Co-fractionation Mus musculus
96 Rpl4 67891
Co-fractionation Mus musculus
97 Otx2  
Affinity Capture-MS Mus musculus
98 Rpl13 270106
Co-fractionation Mus musculus
99 Rpl8 26961
Co-fractionation Mus musculus
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