Gene description for Rpl14
Gene name ribosomal protein L14
Gene symbol Rpl14
Other names/aliases 3100001N19Rik
AA407502
AL022816
CAG-ISL-7
CTG-B33
L14
Species Mus musculus
 Database cross references - Rpl14
ExoCarta ExoCarta_67115
Vesiclepedia VP_67115
Entrez Gene 67115
UniProt Q9CR57  
 Rpl14 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl14
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IEA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
Biological Process
    cytoplasmic translation GO:0002181 NAS
    rRNA processing GO:0006364 ISO
    ribosomal large subunit biogenesis GO:0042273 IBA
    ribosomal large subunit biogenesis GO:0042273 ISO
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    postsynaptic density GO:0014069 ISO
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rpl14 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Rpl28 19943
Co-fractionation Mus musculus
3 Dhx29  
Co-fractionation Mus musculus
4 Elf5  
Affinity Capture-MS Mus musculus
5 Rpl31 114641
Co-fractionation Mus musculus
6 Tubgcp4 51885
Co-fractionation Mus musculus
7 Rps25 75617
Co-fractionation Mus musculus
8 Rps15a 267019
Co-fractionation Mus musculus
9 Tmem173  
Proximity Label-MS Mus musculus
10 Rps10 67097
Co-fractionation Mus musculus
11 Rpl13a 22121
Co-fractionation Mus musculus
12 Rps11 27207
Co-fractionation Mus musculus
13 Rps16 20055
Co-fractionation Mus musculus
14 Rpl15 66480
Co-fractionation Mus musculus
15 Rplp2 67186
Co-fractionation Mus musculus
16 Rpl38  
Co-fractionation Mus musculus
17 Rps4x 20102
Co-fractionation Mus musculus
18 Rpl4 67891
Co-fractionation Mus musculus
19 Rps2 16898
Co-fractionation Mus musculus
20 Rpl11 67025
Co-fractionation Mus musculus
21 Rpl32 19951
Co-fractionation Mus musculus
22 Rps8 20116
Co-fractionation Mus musculus
23 Rpl7a 27176
Co-fractionation Mus musculus
24 Btf3  
Co-fractionation Mus musculus
25 Rps18 20084
Co-fractionation Mus musculus
26 Rpl27 19942
Co-fractionation Mus musculus
27 Atxn1  
Proximity Label-MS Mus musculus
28 Rpl6 19988
Co-fractionation Mus musculus
29 Kctd13  
Affinity Capture-MS Mus musculus
30 Rps19 20085
Co-fractionation Mus musculus
31 Rps26 27370
Co-fractionation Mus musculus
32 Tfe3  
Affinity Capture-MS Mus musculus
33 Rpl24 68193
Co-fractionation Mus musculus
34 Tpt1 22070
Co-fractionation Mus musculus
35 Rps24 20088
Co-fractionation Mus musculus
36 Rpl18a 76808
Co-fractionation Mus musculus
37 Rps7 20115
Co-fractionation Mus musculus
38 Rpl17 319195
Co-fractionation Mus musculus
39 Rpl22 19934
Co-fractionation Mus musculus
40 Rpl5 100503670
Co-fractionation Mus musculus
41 Uggt1 320011
Co-fractionation Mus musculus
42 Fzr1  
Affinity Capture-MS Mus musculus
43 Eef2 13629
Co-fractionation Mus musculus
44 Rps20 67427
Co-fractionation Mus musculus
45 Raver1  
Co-fractionation Mus musculus
46 Rps13 68052
Co-fractionation Mus musculus
47 Rpl12 269261
Co-fractionation Mus musculus
48 Rpl18 19899
Co-fractionation Mus musculus
49 Rpl21 19933
Co-fractionation Mus musculus
50 Rps21 66481
Co-fractionation Mus musculus
51 Rpl7 19989
Co-fractionation Mus musculus
52 Rpl39 67248
Co-fractionation Mus musculus
53 Rps28 54127
Co-fractionation Mus musculus
54 Rplp0 11837
Co-fractionation Mus musculus
55 Rps15 20054
Co-fractionation Mus musculus
56 Eef1a1 13627
Co-fractionation Mus musculus
57 Rps3a1 20091
Co-fractionation Mus musculus
58 Pde12  
Co-fractionation Mus musculus
59 Rpl36a 19982
Co-fractionation Mus musculus
60 Rps9 76846
Co-fractionation Mus musculus
61 Rpl26 19941
Co-fractionation Mus musculus
62 Rpl13 270106
Co-fractionation Mus musculus
63 Rpl23 65019
Co-fractionation Mus musculus
64 Rpl23a 268449
Co-fractionation Mus musculus
65 Rps14 20044
Co-fractionation Mus musculus
66 Rps17 20068
Co-fractionation Mus musculus
67 Rps6 20104
Co-fractionation Mus musculus
68 Rpl30 19946
Co-fractionation Mus musculus
69 Eed  
Affinity Capture-MS Mus musculus
70 Rpsa 16785
Co-fractionation Mus musculus
71 Eef1b2 55949
Co-fractionation Mus musculus
72 Rpl3 27367
Co-fractionation Mus musculus
73 Rpl35 66489
Co-fractionation Mus musculus
74 Fancd2  
Affinity Capture-MS Mus musculus
75 Rpl8 26961
Co-fractionation Mus musculus
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