Gene description for Rpl28
Gene name ribosomal protein L28
Gene symbol Rpl28
Other names/aliases D7Wsu21e
Species Mus musculus
 Database cross references - Rpl28
ExoCarta ExoCarta_19943
Vesiclepedia VP_19943
Entrez Gene 19943
UniProt P41105  
 Rpl28 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl28
Molecular Function
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
Biological Process
    cytoplasmic translation GO:0002181 NAS
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    cell body GO:0044297 IEA
    cell body GO:0044297 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rpl28 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl28
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rpl11 67025
Co-fractionation Mus musculus
2 Rpl35 66489
Co-fractionation Mus musculus
3 Elf5  
Affinity Capture-MS Mus musculus
4 Rpl31 114641
Co-fractionation Mus musculus
5 Fmr1  
Co-fractionation Mus musculus
6 Ddx6 13209
Co-fractionation Mus musculus
7 Rpl15 66480
Co-fractionation Mus musculus
8 Rplp2 67186
Co-fractionation Mus musculus
9 Rpl38  
Co-fractionation Mus musculus
10 Rpl14 67115
Co-fractionation Mus musculus
11 Rpl10a 19896
Co-fractionation Mus musculus
12 Rpl32 19951
Co-fractionation Mus musculus
13 Rps23 66475
Co-fractionation Mus musculus
14 Rpl7a 27176
Co-fractionation Mus musculus
15 Snd1 56463
Co-fractionation Mus musculus
16 Rpl27 19942
Co-fractionation Mus musculus
17 Atxn1  
Proximity Label-MS Mus musculus
18 Kcna3  
Affinity Capture-MS Mus musculus
19 Kctd13  
Affinity Capture-MS Mus musculus
20 Eif4e  
Co-fractionation Mus musculus
21 Tfe3  
Affinity Capture-MS Mus musculus
22 Rpl24 68193
Co-fractionation Mus musculus
23 Rps6 20104
Co-fractionation Mus musculus
24 Ncl 17975
Co-fractionation Mus musculus
25 Rpl17 319195
Co-fractionation Mus musculus
26 Rpl22 19934
Co-fractionation Mus musculus
27 Tmem173  
Proximity Label-MS Mus musculus
28 Fzr1  
Affinity Capture-MS Mus musculus
29 Pabpc1 18458
Co-fractionation Mus musculus
30 Rps11 27207
Co-fractionation Mus musculus
31 Rpl12 269261
Co-fractionation Mus musculus
32 Rpl18 19899
Co-fractionation Mus musculus
33 Rpl21 19933
Co-fractionation Mus musculus
34 Rpl18a 76808
Co-fractionation Mus musculus
35 Rpl7 19989
Co-fractionation Mus musculus
36 Rpl23 65019
Co-fractionation Mus musculus
37 Rock2  
Co-fractionation Mus musculus
38 Rpl36a 19982
Co-fractionation Mus musculus
39 Rpl26 19941
Co-fractionation Mus musculus
40 Rpl13 270106
Co-fractionation Mus musculus
41 Rplp0 11837
Co-fractionation Mus musculus
42 Rpl23a 268449
Co-fractionation Mus musculus
43 Rpl30 19946
Co-fractionation Mus musculus
44 Eed  
Affinity Capture-MS Mus musculus
45 Ndn  
Two-hybrid Mus musculus
46 Nlrp3  
Affinity Capture-MS Mus musculus
47 Rpsa 16785
Co-fractionation Mus musculus
48 Rpl8 26961
Co-fractionation Mus musculus
49 Rpl3 27367
Co-fractionation Mus musculus
50 Stau1  
Co-fractionation Mus musculus
51 Rpl5 100503670
Co-fractionation Mus musculus
52 Fancd2  
Affinity Capture-MS Mus musculus
53 Rpl6 19988
Co-fractionation Mus musculus
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