Gene description for Rpl18
Gene name ribosomal protein L18
Gene symbol Rpl18
Other names/aliases L18
Rpl18a
Species Mus musculus
 Database cross references - Rpl18
ExoCarta ExoCarta_19899
Vesiclepedia VP_19899
Entrez Gene 19899
UniProt P35980  
 Rpl18 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl18
Molecular Function
    RNA binding GO:0003723 IBA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
Biological Process
    cytoplasmic translation GO:0002181 ISO
    cytoplasmic translation GO:0002181 ISS
    cytoplasmic translation GO:0002181 NAS
Subcellular Localization
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 ISO
    rough endoplasmic reticulum GO:0005791 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic large ribosomal subunit GO:0022625 ISS
    cytosolic ribosome GO:0022626 ISO
    synapse GO:0045202 ISO
 Experiment description of studies that identified Rpl18 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl18
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Rpl28 19943
Co-fractionation Mus musculus
3 Rpl35 66489
Co-fractionation Mus musculus
4 Rpl5 100503670
Co-fractionation Mus musculus
5 Rps25 75617
Co-fractionation Mus musculus
6 Rps28 54127
Co-fractionation Mus musculus
7 Rpl11 67025
Co-fractionation Mus musculus
8 Rpl13a 22121
Co-fractionation Mus musculus
9 Acot13  
Cross-Linking-MS (XL-MS) Mus musculus
10 Dhx29  
Co-fractionation Mus musculus
11 Rpsa 16785
Co-fractionation Mus musculus
12 Rplp2 67186
Co-fractionation Mus musculus
13 Rps4x 20102
Co-fractionation Mus musculus
14 Rpl14 67115
Co-fractionation Mus musculus
15 Rps2 16898
Co-fractionation Mus musculus
16 Rpl10a 19896
Co-fractionation Mus musculus
17 Rps23 66475
Co-fractionation Mus musculus
18 Rpl7a 27176
Co-fractionation Mus musculus
19 Raver1  
Co-fractionation Mus musculus
20 Rpl27 19942
Co-fractionation Mus musculus
21 Rpl32 19951
Co-fractionation Mus musculus
22 Kcna3  
Affinity Capture-MS Mus musculus
23 Kctd13  
Affinity Capture-MS Mus musculus
24 Rps19 20085
Co-fractionation Mus musculus
25 Tfe3  
Affinity Capture-MS Mus musculus
26 Rpl9 20005
Co-fractionation Mus musculus
27 Rpl24 68193
Co-fractionation Mus musculus
28 Rps11 27207
Co-fractionation Mus musculus
29 Rpl18a 76808
Co-fractionation Mus musculus
30 Rps24 20088
Co-fractionation Mus musculus
31 Rpl23 65019
Co-fractionation Mus musculus
32 Rpl31 114641
Co-fractionation Mus musculus
33 Rps7 20115
Co-fractionation Mus musculus
34 Rpl17 319195
Co-fractionation Mus musculus
35 Rpl22 19934
Co-fractionation Mus musculus
36 Rpl4 67891
Co-fractionation Mus musculus
37 Fzr1  
Affinity Capture-MS Mus musculus
38 Eef2 13629
Co-fractionation Mus musculus
39 Rps20 67427
Co-fractionation Mus musculus
40 Pa2g4 18813
Co-fractionation Mus musculus
41 Rpl7 19989
Co-fractionation Mus musculus
42 Eef1a1 13627
Co-fractionation Mus musculus
43 Rpl21 19933
Co-fractionation Mus musculus
44 Rps13 68052
Co-fractionation Mus musculus
45 Rpl12 269261
Co-fractionation Mus musculus
46 Rps15a 267019
Co-fractionation Mus musculus
47 Gnb2l1 14694
Co-fractionation Mus musculus
48 Rps15 20054
Co-fractionation Mus musculus
49 Rps5 20103
Co-fractionation Mus musculus
50 Rps3a1 20091
Co-fractionation Mus musculus
51 Rpl36a 19982
Co-fractionation Mus musculus
52 Rps9 76846
Co-fractionation Mus musculus
53 Rpl26 19941
Co-fractionation Mus musculus
54 Rpl13 270106
Co-fractionation Mus musculus
55 Rplp0 11837
Co-fractionation Mus musculus
56 Rpl23a 268449
Co-fractionation Mus musculus
57 Rps14 20044
Co-fractionation Mus musculus
58 Rps17 20068
Co-fractionation Mus musculus
59 Rps6 20104
Co-fractionation Mus musculus
60 Rpl30 19946
Co-fractionation Mus musculus
61 Eed  
Affinity Capture-MS Mus musculus
62 Rpl15 66480
Co-fractionation Mus musculus
63 Eef1b2 55949
Co-fractionation Mus musculus
64 Rpl3 27367
Co-fractionation Mus musculus
65 Rps18 20084
Co-fractionation Mus musculus
66 Rps16 20055
Co-fractionation Mus musculus
67 Rpl6 19988
Co-fractionation Mus musculus
68 Fancd2  
Affinity Capture-MS Mus musculus
69 Rpl8 26961
Co-fractionation Mus musculus
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