Gene description for Gnb2l1
Gene name guanine nucleotide binding protein (G protein), beta polypeptide 2 like 1
Gene symbol Gnb2l1
Other names/aliases AL033335
GB-like
Gnb2-rs1
Rack1
p205
Species Mus musculus
 Database cross references - Gnb2l1
ExoCarta ExoCarta_14694
Vesiclepedia VP_14694
Entrez Gene 14694
UniProt P68040  
 Gnb2l1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gnb2l1
Molecular Function
    protein kinase C binding GO:0005080 IBA
    protein kinase C binding GO:0005080 ISO
    protein kinase C binding GO:0005080 ISS
    protein binding GO:0005515 IPI
    enzyme activator activity GO:0008047 ISO
    ion channel inhibitor activity GO:0008200 IDA
    cysteine-type endopeptidase activator activity involved in apoptotic process GO:0008656 IEA
    cysteine-type endopeptidase activator activity involved in apoptotic process GO:0008656 ISO
    enzyme binding GO:0019899 ISO
    protein phosphatase binding GO:0019903 IEA
    protein phosphatase binding GO:0019903 ISO
    protein tyrosine kinase inhibitor activity GO:0030292 ISO
    protein tyrosine kinase inhibitor activity GO:0030292 ISS
    cyclin binding GO:0030332 IEA
    cyclin binding GO:0030332 ISO
    receptor tyrosine kinase binding GO:0030971 ISO
    receptor tyrosine kinase binding GO:0030971 ISS
    signaling adaptor activity GO:0035591 ISO
    signaling receptor activity GO:0038023 ISO
    SH2 domain binding GO:0042169 ISO
    SH2 domain binding GO:0042169 ISS
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    ribosome binding GO:0043022 IBA
    ribosome binding GO:0043022 ISO
    ribosome binding GO:0043022 ISS
    translation regulator activity GO:0045182 IEA
    BH3 domain binding GO:0051434 IEA
    BH3 domain binding GO:0051434 ISO
Biological Process
    positive regulation of protein phosphorylation GO:0001934 ISO
    positive regulation of protein phosphorylation GO:0001934 ISS
    translation GO:0006412 IMP
    regulation of translation GO:0006417 IEA
    apoptotic process GO:0006915 IEA
    gastrulation GO:0007369 IEA
    protein localization GO:0008104 ISO
    negative regulation of gene expression GO:0010629 IEA
    negative regulation of gene expression GO:0010629 ISO
    protein ubiquitination GO:0016567 ISO
    protein ubiquitination GO:0016567 ISS
    negative regulation of translation GO:0017148 ISO
    negative regulation of Wnt signaling pathway GO:0030178 ISS
    negative regulation of cell growth GO:0030308 ISO
    negative regulation of cell growth GO:0030308 ISS
    positive regulation of cell migration GO:0030335 ISO
    positive regulation of cell migration GO:0030335 ISS
    positive regulation of protein-containing complex assembly GO:0031334 ISO
    positive regulation of protein-containing complex assembly GO:0031334 ISS
    negative regulation of protein binding GO:0032091 ISO
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISO
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISS
    regulation of protein localization GO:0032880 ISS
    negative regulation of peptidyl-serine phosphorylation GO:0033137 ISO
    tumor necrosis factor-mediated signaling pathway GO:0033209 ISO
    positive regulation of amide metabolic process GO:0034250 ISO
    intracellular signal transduction GO:0035556 ISO
    positive regulation of Golgi to plasma membrane protein transport GO:0042998 ISO
    positive regulation of Golgi to plasma membrane protein transport GO:0042998 ISS
    positive regulation of apoptotic process GO:0043065 ISO
    positive regulation of apoptotic process GO:0043065 ISS
    pigmentation GO:0043473 IMP
    positive regulation of GTPase activity GO:0043547 ISO
    positive regulation of GTPase activity GO:0043547 ISS
    positive regulation of lipid metabolic process GO:0045834 ISO
    negative regulation of smoothened signaling pathway GO:0045879 IEA
    negative regulation of smoothened signaling pathway GO:0045879 ISO
    rhythmic process GO:0048511 IEA
    negative regulation of phagocytosis GO:0050765 ISO
    negative regulation of phagocytosis GO:0050765 ISS
    regulation of cell division GO:0051302 ISS
    regulation of cell cycle GO:0051726 ISO
    regulation of cell cycle GO:0051726 ISS
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IEA
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 ISO
    positive regulation of mitochondrial depolarization GO:0051901 IEA
    positive regulation of mitochondrial depolarization GO:0051901 ISO
    cellular response to glucose stimulus GO:0071333 IEA
    cellular response to glucose stimulus GO:0071333 ISO
    cellular response to growth factor stimulus GO:0071363 ISO
    cellular response to growth factor stimulus GO:0071363 ISS
    rescue of stalled ribosome GO:0072344 IBA
    rescue of stalled ribosome GO:0072344 ISO
    rescue of stalled ribosome GO:0072344 ISS
    regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0106070 IEA
    regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0106070 ISO
    regulation of protein localization to plasma membrane GO:1903076 ISO
    negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide GO:1903751 IGI
    negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide GO:1903751 ISO
    regulation of membrane lipid metabolic process GO:1905038 ISO
    regulation of establishment of cell polarity GO:2000114 ISS
    positive regulation of gastrulation GO:2000543 ISS
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
    negative regulation of translational frameshifting GO:2001125 IBA
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 IEA
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISO
Subcellular Localization
    phagocytic cup GO:0001891 ISO
    phagocytic cup GO:0001891 ISS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    small ribosomal subunit GO:0015935 IDA
    small ribosomal subunit GO:0015935 ISO
    dendrite GO:0030425 IDA
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    neuron projection GO:0043005 IDA
    neuronal cell body GO:0043025 IDA
    perikaryon GO:0043204 IEA
    cell body GO:0044297 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
    IRE1-RACK1-PP2A complex GO:1990630 IEA
    IRE1-RACK1-PP2A complex GO:1990630 ISO
 Experiment description of studies that identified Gnb2l1 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gnb2l1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 EEF1G 1937
Two-hybrid Homo sapiens
4 YTHDF1 54915
Two-hybrid Homo sapiens
5 Tfe3  
Affinity Capture-MS Mus musculus
6 Rpl27 19942
Co-fractionation Mus musculus
7 Rpl6 19988
Co-fractionation Mus musculus
8 Rps24 20088
Co-fractionation Mus musculus
9 Dync1li2  
Co-fractionation Mus musculus
10 Gnb1 14688
Affinity Capture-Western Mus musculus
11 Rpl7 19989
Co-fractionation Mus musculus
12 Eef1a1 13627
Co-fractionation Mus musculus
13 Rps9 76846
Co-fractionation Mus musculus
14 Unk  
Affinity Capture-RNA Mus musculus
15 Rpl26 19941
Co-fractionation Mus musculus
16 CPSF7 79869
Two-hybrid Homo sapiens
17 Rpsa 16785
Co-fractionation Mus musculus
18 Rpl3 27367
Co-fractionation Mus musculus
19 Atg16l1  
Affinity Capture-MS Mus musculus
20 Rpl11 67025
Co-fractionation Mus musculus
21 Eed  
Affinity Capture-MS Mus musculus
22 Rpl31 114641
Co-fractionation Mus musculus
23 Rps10 67097
Co-fractionation Mus musculus
24 Stau1  
Co-fractionation Mus musculus
25 Rplp2 67186
Co-fractionation Mus musculus
26 Rps4x 20102
Co-fractionation Mus musculus
27 LARP4B 23185
Two-hybrid Homo sapiens
28 Rps23 66475
Co-fractionation Mus musculus
29 USP10 9100
Two-hybrid Homo sapiens
30 Rpl24 68193
Co-fractionation Mus musculus
31 Rps7 20115
Co-fractionation Mus musculus
32 Pabpc1 18458
Co-fractionation Mus musculus
33 Rps5 20103
Co-fractionation Mus musculus
34 Nanog  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
35 Fancd2  
Affinity Capture-MS Mus musculus
36 Rplp0 11837
Co-fractionation Mus musculus
37 Pgk1 18655
Co-fractionation Mus musculus
38 Rpl30 19946
Co-fractionation Mus musculus
39 Rps26 27370
Co-fractionation Mus musculus
40 Rps16 20055
Co-fractionation Mus musculus
41 Rps17 20068
Co-fractionation Mus musculus
42 Kcna3  
Affinity Capture-MS Mus musculus
43 Nufip2  
Two-hybrid Mus musculus
44 Rps18 20084
Co-fractionation Mus musculus
45 Mycbp2 105689
Affinity Capture-MS Mus musculus
46 Rpl13a 22121
Co-fractionation Mus musculus
47 Rps2 16898
Co-fractionation Mus musculus
48 Rps8 20116
Co-fractionation Mus musculus
49 Rpl7a 27176
Co-fractionation Mus musculus
50 Rpl32 19951
Co-fractionation Mus musculus
51 Rps28 54127
Co-fractionation Mus musculus
52 Rps19 20085
Co-fractionation Mus musculus
53 Tpt1 22070
Co-fractionation Mus musculus
54 Rpl18a 76808
Co-fractionation Mus musculus
55 GNB1 281201
Two-hybrid Bos taurus
Reconstituted Complex Bos taurus
56 Rpl22 19934
Co-fractionation Mus musculus
57 Nedd9  
Affinity Capture-MS Mus musculus
58 Rps6 20104
Co-fractionation Mus musculus
59 Rpl38  
Co-fractionation Mus musculus
60 Rps3a1 20091
Co-fractionation Mus musculus
61 Rps21 66481
Co-fractionation Mus musculus
62 Rps14 20044
Co-fractionation Mus musculus
63 Rpl35 66489
Co-fractionation Mus musculus
64 Rpl5 100503670
Co-fractionation Mus musculus
65 Rps20 67427
Co-fractionation Mus musculus
66 Rps27a 78294
Co-fractionation Mus musculus
67 G3bp2  
Two-hybrid Mus musculus
68 Srp54a  
Two-hybrid Mus musculus
69 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
70 Tsc1  
Affinity Capture-MS Mus musculus
71 SNRPA 6626
Two-hybrid Homo sapiens
72 Htt  
Affinity Capture-MS Mus musculus
73 Prkcd  
Co-fractionation Mus musculus
74 Fzr1  
Affinity Capture-MS Mus musculus
75 Eef2 13629
Co-fractionation Mus musculus
76 Rpl12 269261
Co-fractionation Mus musculus
77 Rpl18 19899
Co-fractionation Mus musculus
78 Rps15 20054
Co-fractionation Mus musculus
79 Rps13 68052
Co-fractionation Mus musculus
80 Rpl23 65019
Co-fractionation Mus musculus
81 Rpl23a 268449
Co-fractionation Mus musculus
82 SNW1 22938
Two-hybrid Homo sapiens
83 G3bp1  
Two-hybrid Mus musculus
84 Rpl15 66480
Co-fractionation Mus musculus
85 Rpl8 26961
Co-fractionation Mus musculus
86 Eef1b2 55949
Co-fractionation Mus musculus
87 Rpl4 67891
Co-fractionation Mus musculus
88 Rpl13 270106
Co-fractionation Mus musculus
89 Ythdf2 213541
Two-hybrid Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here