Gene description for GNB1
Gene name guanine nucleotide binding protein (G protein), beta polypeptide 1
Gene symbol GNB1
Other names/aliases -
Species Bos taurus
 Database cross references - GNB1
ExoCarta ExoCarta_281201
Vesiclepedia VP_281201
Entrez Gene 281201
UniProt P62871  
 GNB1 identified in sEVs derived from the following tissue/cell type
Milk 23459212    
 Gene ontology annotations for GNB1
Molecular Function
    protein binding GO:0005515 IEA
    signaling receptor complex adaptor activity GO:0030159 IEA
Biological Process
    G protein-coupled receptor signaling pathway GO:0007186 IEA
Subcellular Localization
    cytoplasm GO:0005737 IEA
    heterotrimeric G-protein complex GO:0005834 IEA
 Experiment description of studies that identified GNB1 in sEVs
1
Experiment ID 213
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description "Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis."
Authors "Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD."
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DYNC1I1  
Reconstituted Complex Homo sapiens
2 Gnb2l1 14694
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
3 Dync1i1  
Two-hybrid Mus musculus
4 Hdac5  
Two-hybrid Mus musculus
5 Wdr26 226757
Two-hybrid Mus musculus
View the network image/svg+xml
 Pathways in which GNB1 is involved
PathwayEvidenceSource
Activation of G protein gated Potassium channels IEA Reactome
Activation of GABAB receptors IEA Reactome
Activation of kainate receptors upon glutamate binding IEA Reactome
Activation of the phototransduction cascade IEA Reactome
ADP signalling through P2Y purinoceptor 1 IEA Reactome
ADP signalling through P2Y purinoceptor 12 IEA Reactome
Adrenaline,noradrenaline inhibits insulin secretion IEA Reactome
Aquaporin-mediated transport IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Ca2+ pathway IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
Chaperonin-mediated protein folding IEA Reactome
Class B/2 (Secretin family receptors) IEA Reactome
Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding IEA Reactome
ESR-mediated signaling IEA Reactome
Extra-nuclear estrogen signaling IEA Reactome
G alpha (12/13) signalling events IEA Reactome
G alpha (i) signalling events IEA Reactome
G alpha (q) signalling events IEA Reactome
G alpha (s) signalling events IEA Reactome
G alpha (z) signalling events IEA Reactome
G beta:gamma signalling through BTK IEA Reactome
G beta:gamma signalling through CDC42 IEA Reactome
G beta:gamma signalling through PI3Kgamma IEA Reactome
G beta:gamma signalling through PLC beta IEA Reactome
G protein gated Potassium channels IEA Reactome
G-protein activation IEA Reactome
G-protein beta:gamma signalling IEA Reactome
GABA B receptor activation IEA Reactome
GABA receptor activation IEA Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome
Glucagon-type ligand receptors IEA Reactome
GPCR downstream signalling IEA Reactome
GPCR ligand binding IEA Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells IEA Reactome
Incretin synthesis, secretion, and inactivation IEA Reactome
Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits IEA Reactome
Integration of energy metabolism IEA Reactome
Inwardly rectifying K+ channels IEA Reactome
Metabolism IEA Reactome
Metabolism of proteins IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Olfactory Signaling Pathway IEA Reactome
Opioid Signalling IEA Reactome
Peptide hormone metabolism IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Platelet homeostasis IEA Reactome
Potassium Channels IEA Reactome
Presynaptic function of Kainate receptors IEA Reactome
Prostacyclin signalling through prostacyclin receptor IEA Reactome
Protein folding IEA Reactome
Regulation of insulin secretion IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
Sensory Perception IEA Reactome
Sensory perception of sweet, bitter, and umami (glutamate) taste IEA Reactome
Sensory perception of taste IEA Reactome
Signal amplification IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by WNT IEA Reactome
Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) IEA Reactome
The phototransduction cascade IEA Reactome
Thrombin signalling through proteinase activated receptors (PARs) IEA Reactome
Thromboxane signalling through TP receptor IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vasopressin regulates renal water homeostasis via Aquaporins IEA Reactome
Visual phototransduction IEA Reactome





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