Gene description for Pabpc1
Gene name poly(A) binding protein, cytoplasmic 1
Gene symbol Pabpc1
Other names/aliases PABP
Pabp1
PabpI
Pabpl1
ePAB
Species Mus musculus
 Database cross references - Pabpc1
ExoCarta ExoCarta_18458
Vesiclepedia VP_18458
Entrez Gene 18458
UniProt P29341  
 Pabpc1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pabpc1
Molecular Function
    RNA binding GO:0003723 ISO
    mRNA binding GO:0003729 IEA
    mRNA binding GO:0003729 ISO
    mRNA 3'-UTR binding GO:0003730 IBA
    mRNA 3'-UTR binding GO:0003730 ISO
    protein binding GO:0005515 IPI
    poly(A) binding GO:0008143 IBA
    poly(A) binding GO:0008143 ISO
    poly(A) binding GO:0008143 ISS
    poly(U) RNA binding GO:0008266 IBA
    poly(U) RNA binding GO:0008266 ISO
Biological Process
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IEA
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    regulatory ncRNA-mediated gene silencing GO:0031047 IMP
    positive regulation of nuclear-transcribed mRNA poly(A) tail shortening GO:0060213 IMP
    CRD-mediated mRNA stabilization GO:0070934 ISO
    negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900152 ISO
    positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900153 IMP
    negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:2000623 ISS
    positive regulation of cytoplasmic translation GO:2000767 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    spliceosomal complex GO:0005681 IEA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    cytoplasmic stress granule GO:0010494 IBA
    cytoplasmic stress granule GO:0010494 ISO
    cytoplasmic stress granule GO:0010494 ISS
    lamellipodium GO:0030027 IEA
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    cell leading edge GO:0031252 ISO
    cell leading edge GO:0031252 ISS
    protein-containing complex GO:0032991 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    catalytic step 2 spliceosome GO:0071013 ISO
    mCRD-mediated mRNA stability complex GO:0106002 ISO
    ribonucleoprotein complex GO:1990904 IBA
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Pabpc1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pabpc1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LARP1 23367
Affinity Capture-MS Homo sapiens
2 Rpl28 19943
Co-fractionation Mus musculus
3 Fxr1 14359
Affinity Capture-MS Mus musculus
4 Ubc  
Reconstituted Complex Mus musculus
5 Rps18 20084
Co-fractionation Mus musculus
6 Prmt5 27374
Biochemical Activity Mus musculus
7 MKRN1 23608
Affinity Capture-MS Homo sapiens
8 Sra1  
Co-fractionation Mus musculus
9 Sarnp 66118
Co-fractionation Mus musculus
10 DNAJC21  
Affinity Capture-MS Homo sapiens
11 Stau1  
Co-fractionation Mus musculus
12 PABPC4 8761
Affinity Capture-MS Homo sapiens
13 Rbm38  
Two-hybrid Mus musculus
14 Gnb2l1 14694
Co-fractionation Mus musculus
15 Rps4x 20102
Co-fractionation Mus musculus
16 LARP4B 23185
Affinity Capture-MS Homo sapiens
17 Rac1 19353
Co-fractionation Mus musculus
18 EIF4G1 1981
Affinity Capture-MS Homo sapiens
19 ASCC1 51008
Affinity Capture-MS Homo sapiens
20 Rps8 20116
Co-fractionation Mus musculus
21 RPS23 6228
Affinity Capture-MS Homo sapiens
22 Tmem173  
Proximity Label-MS Mus musculus
23 Mapt  
Co-fractionation Mus musculus
24 Rpl6 19988
Co-fractionation Mus musculus
25 Kctd13  
Affinity Capture-MS Mus musculus
26 Rplp2 67186
Co-fractionation Mus musculus
27 Snrnp200 320632
Co-fractionation Mus musculus
28 Atg16l1  
Affinity Capture-MS Mus musculus
29 Snrpd2 107686
Co-fractionation Mus musculus
30 Sf3a1 67465
Co-fractionation Mus musculus
31 Rps6 20104
Co-fractionation Mus musculus
32 Snrpd3 67332
Co-fractionation Mus musculus
33 Tpt1 22070
Co-fractionation Mus musculus
34 Hnrnpl 15388
Co-fractionation Mus musculus
35 Sf3b3 101943
Co-fractionation Mus musculus
36 EXOSC8  
Two-hybrid Homo sapiens
37 Rbm8a  
Co-fractionation Mus musculus
38 GZMB  
Biochemical Activity Homo sapiens
39 Fzr1  
Affinity Capture-MS Mus musculus
40 Upf1 19704
Co-fractionation Mus musculus
41 Rpl26 19941
Co-fractionation Mus musculus
42 MRPS18B 28973
Affinity Capture-MS Homo sapiens
43 Rock2  
Co-fractionation Mus musculus
44 Rps9 76846
Co-fractionation Mus musculus
45 Ctr9  
Affinity Capture-MS Mus musculus
46 Sf3b1 81898
Co-fractionation Mus musculus
47 Srrm2  
Co-fractionation Mus musculus
48 Ina  
Co-fractionation Mus musculus
49 Dmrt2  
Affinity Capture-MS Mus musculus
50 Atg12  
Negative Genetic Mus musculus
51 Nanog  
Affinity Capture-MS Mus musculus
52 Rptor  
Co-fractionation Mus musculus
53 Rps3 27050
Co-fractionation Mus musculus
54 Rps3a1 20091
Co-fractionation Mus musculus
55 Ssb 20823
Co-fractionation Mus musculus
56 Dhx15 13204
Co-fractionation Mus musculus
57 Ctsh  
Co-fractionation Mus musculus
58 VKORC1 79001
Affinity Capture-MS Homo sapiens
59 Ybx1 22608
Co-fractionation Mus musculus
60 Tubb5 22154
Co-fractionation Mus musculus
61 Tdrd3  
Co-fractionation Mus musculus
62 ZC3HAV1 56829
Affinity Capture-MS Homo sapiens
63 Eed  
Affinity Capture-MS Mus musculus
64 Syncrip 56403
Co-fractionation Mus musculus
65 Snrpd1 20641
Co-fractionation Mus musculus
66 Paip1  
Affinity Capture-Western Mus musculus
67 CETN2 1069
Affinity Capture-MS Homo sapiens
68 Tia1  
Affinity Capture-MS Mus musculus
Co-fractionation Mus musculus
69 SND1 27044
Affinity Capture-MS Homo sapiens
70 Poldip3  
Co-fractionation Mus musculus
71 Rpl4 67891
Co-fractionation Mus musculus
72 LARP4 113251
Affinity Capture-MS Homo sapiens
73 Otx2  
Affinity Capture-MS Mus musculus
74 Fancd2  
Affinity Capture-MS Mus musculus
75 Rps28 54127
Co-fractionation Mus musculus
76 PAIP1 10605
Affinity Capture-MS Homo sapiens
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