Gene description for Sf3a1
Gene name splicing factor 3a, subunit 1
Gene symbol Sf3a1
Other names/aliases 1200014H24Rik
5930416L09Rik
AI159724
Species Mus musculus
 Database cross references - Sf3a1
ExoCarta ExoCarta_67465
Vesiclepedia VP_67465
Entrez Gene 67465
UniProt Q8K4Z5  
 Sf3a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sf3a1
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 ISO
    RNA binding GO:0003723 ISS
    protein binding GO:0005515 IPI
Biological Process
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    mRNA processing GO:0006397 ISO
    mRNA cis splicing, via spliceosome GO:0045292 IEA
    U2-type prespliceosome assembly GO:1903241 ISO
    U2-type prespliceosome assembly GO:1903241 ISS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 ISO
    U2-type spliceosomal complex GO:0005684 ISO
    U2-type spliceosomal complex GO:0005684 ISS
    U2 snRNP GO:0005686 IBA
    U2 snRNP GO:0005686 ISO
    U2 snRNP GO:0005686 ISS
    nuclear speck GO:0016607 ISO
    nuclear speck GO:0016607 ISS
    U2-type prespliceosome GO:0071004 IBA
    U2-type prespliceosome GO:0071004 ISO
    U2-type prespliceosome GO:0071004 ISS
    U2-type precatalytic spliceosome GO:0071005 ISO
    U2-type precatalytic spliceosome GO:0071005 ISS
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Sf3a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sf3a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 P3H1 64175
Affinity Capture-MS Homo sapiens
2 SNRPB2 6629
Affinity Capture-MS Homo sapiens
3 DDX46 9879
Affinity Capture-MS Homo sapiens
4 DHX15 1665
Affinity Capture-MS Homo sapiens
5 Smek1  
Co-fractionation Mus musculus
6 SF3B2 10992
Affinity Capture-MS Homo sapiens
7 RBM39 9584
Affinity Capture-MS Homo sapiens
8 SNRPG 6637
Affinity Capture-MS Homo sapiens
9 Snrpd1 20641
Co-fractionation Mus musculus
10 SNRPE 6635
Affinity Capture-MS Homo sapiens
11 DNAJC8 22826
Affinity Capture-MS Homo sapiens
12 TTC33  
Affinity Capture-MS Homo sapiens
13 WDR61 80349
Affinity Capture-MS Homo sapiens
14 CHERP 10523
Affinity Capture-MS Homo sapiens
15 Hnrnph1 59013
Co-fractionation Mus musculus
16 SF3A1 10291
Affinity Capture-MS Homo sapiens
17 SNRPA1 6627
Affinity Capture-MS Homo sapiens
18 SNRPB 6628
Affinity Capture-MS Homo sapiens
19 SF3B4 10262
Affinity Capture-MS Homo sapiens
20 HTATSF1 27336
Affinity Capture-MS Homo sapiens
21 SMURF1 57154
Affinity Capture-Luminescence Homo sapiens
22 Dnajc8  
Co-fractionation Mus musculus
23 Hnrnpu 51810
Co-fractionation Mus musculus
24 Chaf1a  
Affinity Capture-MS Mus musculus
25 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
26 CCDC97  
Affinity Capture-MS Homo sapiens
27 SNRPF 6636
Affinity Capture-MS Homo sapiens
28 SNRPD2 6633
Affinity Capture-MS Homo sapiens
29 SF3B5 83443
Affinity Capture-MS Homo sapiens
30 Sf3b3 101943
Co-fractionation Mus musculus
31 Tcerg1  
Co-fractionation Mus musculus
32 Lsm3  
Co-fractionation Mus musculus
33 Pabpc1 18458
Co-fractionation Mus musculus
34 SNRPD1 6632
Affinity Capture-MS Homo sapiens
35 Brcc3  
Co-fractionation Mus musculus
36 CRTAP 10491
Affinity Capture-MS Homo sapiens
37 Syncrip 56403
Co-fractionation Mus musculus
38 SF3B1 23451
Affinity Capture-MS Homo sapiens
39 SF3A3 10946
Affinity Capture-MS Homo sapiens
40 PHF5A 84844
Affinity Capture-MS Homo sapiens
41 DDX42 11325
Affinity Capture-MS Homo sapiens
42 SF3B6 51639
Affinity Capture-MS Homo sapiens
43 SF3B3 23450
Affinity Capture-MS Homo sapiens
44 Ddx42 72047
Co-fractionation Mus musculus
45 Eed  
Affinity Capture-MS Mus musculus
46 Cwc27  
Co-fractionation Mus musculus
47 U2af2 22185
Co-fractionation Mus musculus
48 SF3A2 8175
Affinity Capture-MS Homo sapiens
49 Srrm2  
Co-fractionation Mus musculus
50 Ccdc6  
Co-fractionation Mus musculus
51 RBM17 84991
Affinity Capture-MS Homo sapiens
52 Hnrnpa2b1 53379
Co-fractionation Mus musculus
53 GPATCH11  
Affinity Capture-MS Homo sapiens
54 SNRPD3 6634
Affinity Capture-MS Homo sapiens
55 U2SURP 23350
Affinity Capture-MS Homo sapiens
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