Gene description for Prmt1
Gene name protein arginine N-methyltransferase 1
Gene symbol Prmt1
Other names/aliases 6720434D09Rik
AW214366
Hrmt1l2
Mrmt1
Species Mus musculus
 Database cross references - Prmt1
ExoCarta ExoCarta_15469
Vesiclepedia VP_15469
Entrez Gene 15469
UniProt Q9JIF0  
 Prmt1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prmt1
Molecular Function
    protein binding GO:0005515 IPI
    N-methyltransferase activity GO:0008170 ISO
    N-methyltransferase activity GO:0008170 ISS
    protein methyltransferase activity GO:0008276 IMP
    protein methyltransferase activity GO:0008276 ISO
    methyl-CpG binding GO:0008327 ISO
    methyl-CpG binding GO:0008327 ISS
    protein-arginine N-methyltransferase activity GO:0016274 IDA
    protein-arginine N-methyltransferase activity GO:0016274 ISO
    enzyme binding GO:0019899 ISO
    snoRNP binding GO:0030519 ISO
    protein-arginine omega-N monomethyltransferase activity GO:0035241 IBA
    protein-arginine omega-N monomethyltransferase activity GO:0035241 ISO
    protein-arginine omega-N monomethyltransferase activity GO:0035241 ISS
    protein-arginine omega-N asymmetric methyltransferase activity GO:0035242 IBA
    protein-arginine omega-N asymmetric methyltransferase activity GO:0035242 IDA
    protein-arginine omega-N asymmetric methyltransferase activity GO:0035242 ISO
    histone H3R17 methyltransferase activity GO:0035642 IEA
    histone methyltransferase activity GO:0042054 IBA
    histone methyltransferase activity GO:0042054 IDA
    histone methyltransferase activity GO:0042054 ISO
    identical protein binding GO:0042802 ISO
    identical protein binding GO:0042802 ISS
    histone H4R3 methyltransferase activity GO:0044020 ISO
    histone H4R3 methyltransferase activity GO:0044020 ISS
    mitogen-activated protein kinase p38 binding GO:0048273 IEA
    mitogen-activated protein kinase p38 binding GO:0048273 ISO
    histone H3K37 methyltransferase activity GO:0062122 IEA
    histone H3R2 methyltransferase activity GO:0070611 IEA
    GATOR1 complex binding GO:0106080 IDA
    histone H3R8 methyltransferase activity GO:0140592 IEA
    histone H3K56 methyltransferase activity GO:0140759 IEA
    histone H3R26 methyltransferase activity GO:0140903 IEA
    histone H4K12 methyltransferase activity GO:0140984 IEA
    S-adenosyl-L-methionine binding GO:1904047 ISO
    S-adenosyl-L-methionine binding GO:1904047 ISS
    histone H2AQ104 methyltransferase activity GO:1990259 IEA
Biological Process
    in utero embryonic development GO:0001701 IMP
    chromatin remodeling GO:0006338 IBA
    chromatin remodeling GO:0006338 IEA
    regulation of DNA-templated transcription GO:0006355 IBA
    protein methylation GO:0006479 ISO
    protein methylation GO:0006479 ISO
    DNA damage response GO:0006974 IEA
    DNA damage response GO:0006974 ISO
    positive regulation of cell population proliferation GO:0008284 ISO
    positive regulation of cell population proliferation GO:0008284 ISS
    RNA splicing GO:0008380 IMP
    peptidyl-arginine methylation GO:0018216 ISO
    peptidyl-arginine methylation, to asymmetrical-dimethyl arginine GO:0019919 ISO
    regulation of BMP signaling pathway GO:0030510 IEA
    regulation of BMP signaling pathway GO:0030510 ISO
    neuron projection development GO:0031175 IEA
    neuron projection development GO:0031175 ISO
    methylation GO:0032259 IEA
    peptidyl-arginine omega-N-methylation GO:0035247 ISO
    positive regulation of erythrocyte differentiation GO:0045648 IEA
    positive regulation of erythrocyte differentiation GO:0045648 ISO
    regulation of megakaryocyte differentiation GO:0045652 ISO
    regulation of megakaryocyte differentiation GO:0045652 ISS
    negative regulation of megakaryocyte differentiation GO:0045653 ISO
    negative regulation of megakaryocyte differentiation GO:0045653 ISS
    positive regulation of translation GO:0045727 IEA
    positive regulation of translation GO:0045727 ISO
    negative regulation of JNK cascade GO:0046329 ISO
    negative regulation of JNK cascade GO:0046329 ISS
    cardiac muscle tissue development GO:0048738 IMP
    protein homooligomerization GO:0051260 ISO
    protein homooligomerization GO:0051260 ISS
    cellular response to methionine GO:0061431 IMP
    cellular response to methionine GO:0061431 ISO
    positive regulation of TORC1 signaling GO:1904263 IMP
    positive regulation of TORC1 signaling GO:1904263 ISO
    positive regulation of double-strand break repair via homologous recombination GO:1905168 ISO
    positive regulation of double-strand break repair via homologous recombination GO:1905168 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    protein-containing complex GO:0032991 ISO
    methylosome GO:0034709 ISO
    methylosome GO:0034709 ISS
 Experiment description of studies that identified Prmt1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prmt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Elp2  
Co-fractionation Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 Trmt11  
Co-fractionation Mus musculus
4 Tsr2  
Co-fractionation Mus musculus
5 Zfp42  
Affinity Capture-MS Mus musculus
6 Nle1  
Co-fractionation Mus musculus
7 Wdr12  
Co-fractionation Mus musculus
8 Nop56 67134
Affinity Capture-MS Mus musculus
9 Rbm8a  
Co-fractionation Mus musculus
10 Fmr1  
Affinity Capture-MS Mus musculus
11 Rbmxl1 19656
Affinity Capture-MS Mus musculus
12 Runx1 12394
Affinity Capture-MS Mus musculus
13 Rsl1d1 66409
Co-fractionation Mus musculus
14 Hnrnpu 51810
Affinity Capture-MS Mus musculus
15 Pus1  
Co-fractionation Mus musculus
16 Chtop  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Biochemical Activity Mus musculus
17 Lsm14a  
Affinity Capture-MS Mus musculus
18 Serbp1 66870
Affinity Capture-MS Mus musculus
19 Ddx47  
Co-fractionation Mus musculus
20 Polr1c  
Co-fractionation Mus musculus
21 Ppp6r3  
Co-fractionation Mus musculus
22 Zc3h15 69082
Co-fractionation Mus musculus
23 Ybx3  
Affinity Capture-MS Mus musculus
24 Atxn1  
Affinity Capture-MS Mus musculus
25 Nr0b1  
Affinity Capture-MS Mus musculus
26 Mettl1  
Co-fractionation Mus musculus
27 Fancd2  
Affinity Capture-MS Mus musculus
28 Pwp1  
Co-fractionation Mus musculus
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