Gene description for Sfpq
Gene name splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated)
Gene symbol Sfpq
Other names/aliases 1110004P21Rik
2810416M14Rik
5730453G22Rik
9030402K04Rik
AU021830
D4Ertd314e
Gm12940
OTTMUSG00000009329
PSF
REP1
Species Mus musculus
 Database cross references - Sfpq
ExoCarta ExoCarta_71514
Vesiclepedia VP_71514
Entrez Gene 71514
UniProt Q8VIJ6  
 Sfpq identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sfpq
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    DNA binding GO:0003677 ISO
    chromatin binding GO:0003682 IDA
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 ISO
    histone deacetylase binding GO:0042826 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    alternative mRNA splicing, via spliceosome GO:0000380 IBA
    alternative mRNA splicing, via spliceosome GO:0000380 ISO
    double-strand break repair via homologous recombination GO:0000724 ISO
    activation of innate immune response GO:0002218 ISO
    chromatin remodeling GO:0006338 IMP
    regulation of DNA-templated transcription GO:0006355 IBA
    mRNA processing GO:0006397 IEA
    DNA damage response GO:0006974 IMP
    RNA splicing GO:0008380 IEA
    regulation of circadian rhythm GO:0042752 IMP
    negative regulation of circadian rhythm GO:0042754 IMP
    innate immune response GO:0045087 IEA
    positive regulation of sister chromatid cohesion GO:0045876 IMP
    negative regulation of DNA-templated transcription GO:0045892 IDA
    negative regulation of DNA-templated transcription GO:0045892 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    rhythmic process GO:0048511 IEA
    chromosome organization GO:0051276 IMP
    regulation of cell cycle GO:0051726 IMP
    dendritic transport of messenger ribonucleoprotein complex GO:0098963 IDA
    dendritic transport of messenger ribonucleoprotein complex GO:0098963 IMP
    positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902177 ISO
Subcellular Localization
    chromatin GO:0000785 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IEA
    nuclear matrix GO:0016363 IEA
    nuclear matrix GO:0016363 ISO
    nuclear speck GO:0016607 IEA
    dendrite GO:0030425 IEA
    paraspeckles GO:0042382 IDA
    paraspeckles GO:0042382 ISO
    RNA polymerase II transcription regulator complex GO:0090575 IDA
 Experiment description of studies that identified Sfpq in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sfpq
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ddx5 13207
Co-fractionation Mus musculus
2 Ppp1ca 19045
Co-fractionation Mus musculus
3 Snrnp200 320632
Co-fractionation Mus musculus
4 Trim28 21849
Co-fractionation Mus musculus
5 Pfkl 18641
Co-fractionation Mus musculus
6 Hspa8 15481
Co-fractionation Mus musculus
7 Snrpd1 20641
Co-fractionation Mus musculus
8 Srrm1 51796
Co-fractionation Mus musculus
9 Fus  
Co-fractionation Mus musculus
10 Cdc5l 71702
Co-fractionation Mus musculus
11 Sin3a  
Affinity Capture-Western Mus musculus
12 Sf3b1 81898
Co-fractionation Mus musculus
13 Tsc1  
Affinity Capture-MS Mus musculus
14 Pspc1  
Co-fractionation Mus musculus
15 Atxn1  
Proximity Label-MS Mus musculus
16 Kctd13  
Affinity Capture-MS Mus musculus
17 Smarca2  
Affinity Capture-MS Mus musculus
18 Tfe3  
Affinity Capture-MS Mus musculus
19 Hnrnpu 51810
Co-fractionation Mus musculus
20 Snrpd2 107686
Co-fractionation Mus musculus
21 Ddb1 13194
Co-fractionation Mus musculus
22 Ywhaz 22631
Affinity Capture-MS Mus musculus
23 Snrpd3 67332
Co-fractionation Mus musculus
24 Caprin1 53872
Co-fractionation Mus musculus
25 Mettl21e  
Affinity Capture-MS Mus musculus
26 Srsf1 110809
Co-fractionation Mus musculus
27 Eef1a1 13627
Co-fractionation Mus musculus
28 Per1  
Affinity Capture-MS Mus musculus
29 Unk  
Affinity Capture-RNA Mus musculus
30 Snrpa  
Co-fractionation Mus musculus
31 Snw1  
Co-fractionation Mus musculus
32 Sfpq 71514
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
33 Fancd2  
Affinity Capture-MS Mus musculus
34 Ctr9  
Affinity Capture-MS Mus musculus
35 Eed  
Affinity Capture-MS Mus musculus
36 Hspa5 14828
Co-fractionation Mus musculus
37 Ywhae 22627
Affinity Capture-MS Mus musculus
38 U2af2 22185
Co-fractionation Mus musculus
39 ELAVL4  
Reconstituted Complex Homo sapiens
40 Smarca4  
Affinity Capture-MS Mus musculus
41 Tcerg1  
Co-fractionation Mus musculus
42 Otx2  
Affinity Capture-MS Mus musculus
43 Eftud2 20624
Co-fractionation Mus musculus
44 Per2  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
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