Experiment description of studies that identified Ddx5 in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Ddx5

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Sf3a3 75062 Co-fractionation Mus musculus 2 Creb3l4   Affinity Capture-MS Mus musculus 3 Kctd13   Affinity Capture-MS Mus musculus 4 Prpf8 192159 Co-fractionation Mus musculus 5 Rybp   Affinity Capture-Western Mus musculus 6 Snrnp200 320632 Co-fractionation Mus musculus 7 Nyap2   Co-fractionation Mus musculus 8 Smad7   Affinity Capture-Western Mus musculus 9 Sf3b3 101943 Co-fractionation Mus musculus 10 Prpf40a   Co-fractionation Mus musculus 11 Hnrnpc 15381 Co-fractionation Mus musculus 12 Prpf4b 19134 Co-fractionation Mus musculus 13 Nupr1   Reconstituted Complex Mus musculus Two-hybrid Mus musculus Co-localization Mus musculus 14 Pou5f1   Affinity Capture-Western Mus musculus 15 Smu1   Co-fractionation Mus musculus 16 Smad4 17128 Affinity Capture-Western Mus musculus 17 Sf3b1 81898 Co-fractionation Mus musculus 18 Ttc5   Co-fractionation Mus musculus 19 Smad3   Affinity Capture-Western Mus musculus Affinity Capture-Western Mus musculus 20 Rbm39 170791 Co-fractionation Mus musculus 21 Fzr1   Affinity Capture-MS Mus musculus 22 Cbx7   Affinity Capture-Western Mus musculus 23 Smad2   Affinity Capture-Western Mus musculus 24 Stau2   Co-fractionation Mus musculus 25 Tfe3   Affinity Capture-MS Mus musculus 26 Cul3   Affinity Capture-MS Mus musculus 27 Skiv2l2   Co-fractionation Mus musculus 28 Ncl 17975 Co-fractionation Mus musculus 29 Snrnp27   Co-fractionation Mus musculus 30 Mecp2   Affinity Capture-MS Mus musculus 31 Rbm8a   Co-fractionation Mus musculus 32 Lsm7 66094 Co-fractionation Mus musculus 33 Prpf3 70767 Co-fractionation Mus musculus 34 Hnrnph1 59013 Affinity Capture-Western Mus musculus 35 Tmem173   Proximity Label-MS Mus musculus 36 Rnf2   Affinity Capture-Western Mus musculus 37 Tardbp 230908 Affinity Capture-Western Mus musculus Co-fractionation Mus musculus 38 Thoc2   Co-fractionation Mus musculus 39 Atg12   Positive Genetic Mus musculus 40 Hnrnpm 76936 Co-fractionation Mus musculus 41 2310036O22Rik   Co-fractionation Mus musculus 42 Srrm2   Co-fractionation Mus musculus 43 Tfip11   Co-fractionation Mus musculus 44 Unk   Affinity Capture-RNA Mus musculus 45 Syncrip 56403 Co-fractionation Mus musculus 46 Snw1   Co-fractionation Mus musculus 47 Ppwd1   Co-fractionation Mus musculus 48 Npm1 18148 Affinity Capture-MS Mus musculus 49 Eftud2 20624 Co-fractionation Mus musculus 50 Hnrnpdl 50926 Co-fractionation Mus musculus 51 Snrpa1 68981 Co-fractionation Mus musculus 52 Ppil3   Co-fractionation Mus musculus 53 Mapkapk2   Two-hybrid Mus musculus 54 Eed   Affinity Capture-MS Mus musculus 55 Prpf18   Co-fractionation Mus musculus 56 Cwc27   Co-fractionation Mus musculus 57 Drosha   Affinity Capture-Western Mus musculus 58 Cactin   Co-fractionation Mus musculus 59 Mapt   Affinity Capture-MS Mus musculus 60 Tia1   Affinity Capture-MS Mus musculus Affinity Capture-Western Mus musculus 61 Eif4a3 192170 Co-fractionation Mus musculus 62 Sfpq 71514 Co-fractionation Mus musculus 63 Smad1   Affinity Capture-Western Mus musculus 64 Otx2   Affinity Capture-MS Mus musculus 65 Fancd2   Affinity Capture-MS Mus musculus 66 Tcf3   Affinity Capture-MS Mus musculus View the network

Pathways in which Ddx5 is involved

No pathways found